################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII AccIII AcyI AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I AspEI AviII BamHI BbeI BcgI BfrI BglII BsaHI BseAI BsiEI BsiWI BsmBI BspEI BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI Hin1I HpaI KasI KspI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PinAI PmeI PshBI PvuI RsrII SacII SalI SbfI SexAI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI XbaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 1884 GGTACC G^GTACC AccI 2010 GTCTAC GT^[AC][GT]AC AccII 3723 CGCG CG^CG AclI 3935 AACGTT AA^CGTT Alw44I 3596 GTGCAC G^TGCAC ApaLI 3596 GTGCAC G^TGCAC Asp718I 1884 GGTACC G^GTACC AvrII 1455 CCTAGG C^CTAGG BbrPI 513 CACGTG CAC^GTG BciVI 2131 GTATCC GTATCC(6/5) BclI 1840 TGATCA T^GATCA BglI 325 GCCAGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1455 CCTAGG C^CTAGG BlpI 1548 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1548 GCTCAGC GC^T[ACGT]AGC BsaAI 513 CACGTG [CT]AC^GT[GA] BsaWI 3976 TCCGGT [AT]^CCGG[AT] Bsp1407I 2078 TGTACA T^GTACA BspDI 2958 ATCGAT AT^CGAT BspHI 1156 TCATGA T^CATGA BsrGI 2078 TGTACA T^GTACA BstAPI 177 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 3723 CGCG CG^CG CelII 1548 GCTCAGC GC^T[ACGT]AGC ClaI 2958 ATCGAT AT^CGAT EcoT22I 4154 ATGCAT ATGCA^T FbaI 1840 TGATCA T^GATCA HindIII 2994 AAGCTT A^AGCTT KpnI 1884 GGTACC GGTAC^C MvnI 3723 CGCG CG^CG NcoI 2662 CCATGG C^CATGG NsiI 4154 ATGCAT ATGCA^T PaeR7I 649 CTCGAG C^TCGAG PmaCI 513 CACGTG CAC^GTG PmlI 513 CACGTG CAC^GTG Psp1406I 3935 AACGTT AA^CGTT RcaI 1156 TCATGA T^CATGA SacI 1428 GAGCTC GAGCT^C SapI 2766 GAAGAGC GCTCTTC(1/4) ScaI 2124 AGTACT AGT^ACT SfiI 324 GGCCAGTTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 545 CCCGGG CCC^GGG SspBI 2078 TGTACA T^GTACA StuI 4082 AGGCCT AGG^CCT XhoI 649 CTCGAG C^TCGAG XmaCI 545 CCCGGG C^CCGGG XmaI 545 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AocI 268 CCTCAGG CC^T[ACGT]AGG 3345 CCTCAGG ApaI 564 GGGCCC GGGCC^C 3449 GGGCCC AspI 2714 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3284 GACACCGTC BsaBI 976 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2361 GATCTGTATC BsaI 2676 GAGACC GGTCTC(1/5) 2952 GGTCTC BsmI 953 GAATGC GAATGC(1/-1) 2980 GAATGC BspLU11I 1144 ACATGT A^CATGT 1585 ACATGT BspMI 173 ACCTGC ACCTGC(4/8) 2065 ACCTGC BsrFI 1251 ACCGGC [GA]^CCGG[CT] 2242 ACCGGC Bsu36I 268 CCTCAGG CC^T[ACGT]AGG 3345 CCTCAGG Cfr10I 1251 ACCGGC [GA]^CCGG[CT] 2242 ACCGGC DraIII 2468 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG 3757 CACTTCGTG DsaI 2662 CCATGG C^C[GA][CT]GG 4074 CCGTGG Eco81I 268 CCTCAGG CC^T[ACGT]AGG 3345 CCTCAGG EcoRI 1305 GAATTC G^AATTC 2226 GAATTC FauI 964 CCCGC CCCGC(4/6) 2111 GCGGG MamI 976 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2361 GATCTGTATC NspI 1144 ACATGT [GA]CATG^[CT] 1585 ACATGT PflFI 2714 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3284 GACACCGTC Tth111I 2714 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 3284 GACACCGTC ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV PflMI PleI PpuMI PshAI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII