##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII AccIII AcyI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
Asp700I AspEI AviII BamHI BbeI
BcgI BfrI BglII BsaHI BseAI
BsiEI BsiWI BsmBI BspEI BsrBI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRV FseI FspI Hin1I
HpaI KasI KspI MfeI MluI
MroI MunI NaeI NarI NgoMIV
NheI NotI NruI NspV PacI
PinAI PmeI PshBI PvuI RsrII
SacII SalI SbfI SexAI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 1884 GGTACC G^GTACC
AccI 2010 GTCTAC GT^[AC][GT]AC
AccII 3723 CGCG CG^CG
AclI 3935 AACGTT AA^CGTT
Alw44I 3596 GTGCAC G^TGCAC
ApaLI 3596 GTGCAC G^TGCAC
Asp718I 1884 GGTACC G^GTACC
AvrII 1455 CCTAGG C^CTAGG
BbrPI 513 CACGTG CAC^GTG
BciVI 2131 GTATCC GTATCC(6/5)
BclI 1840 TGATCA T^GATCA
BglI 325 GCCAGTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1455 CCTAGG C^CTAGG
BlpI 1548 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1548 GCTCAGC GC^T[ACGT]AGC
BsaAI 513 CACGTG [CT]AC^GT[GA]
BsaWI 3976 TCCGGT [AT]^CCGG[AT]
Bsp1407I 2078 TGTACA T^GTACA
BspDI 2958 ATCGAT AT^CGAT
BspHI 1156 TCATGA T^CATGA
BsrGI 2078 TGTACA T^GTACA
BstAPI 177 GCAGCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 3723 CGCG CG^CG
CelII 1548 GCTCAGC GC^T[ACGT]AGC
ClaI 2958 ATCGAT AT^CGAT
EcoT22I 4154 ATGCAT ATGCA^T
FbaI 1840 TGATCA T^GATCA
HindIII 2994 AAGCTT A^AGCTT
KpnI 1884 GGTACC GGTAC^C
MvnI 3723 CGCG CG^CG
NcoI 2662 CCATGG C^CATGG
NsiI 4154 ATGCAT ATGCA^T
PaeR7I 649 CTCGAG C^TCGAG
PmaCI 513 CACGTG CAC^GTG
PmlI 513 CACGTG CAC^GTG
Psp1406I 3935 AACGTT AA^CGTT
RcaI 1156 TCATGA T^CATGA
SacI 1428 GAGCTC GAGCT^C
SapI 2766 GAAGAGC GCTCTTC(1/4)
ScaI 2124 AGTACT AGT^ACT
SfiI 324 GGCCAGTTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 545 CCCGGG CCC^GGG
SspBI 2078 TGTACA T^GTACA
StuI 4082 AGGCCT AGG^CCT
XhoI 649 CTCGAG C^TCGAG
XmaCI 545 CCCGGG C^CCGGG
XmaI 545 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AocI 268 CCTCAGG CC^T[ACGT]AGG
3345 CCTCAGG
ApaI 564 GGGCCC GGGCC^C
3449 GGGCCC
AspI 2714 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3284 GACACCGTC
BsaBI 976 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2361 GATCTGTATC
BsaI 2676 GAGACC GGTCTC(1/5)
2952 GGTCTC
BsmI 953 GAATGC GAATGC(1/-1)
2980 GAATGC
BspLU11I 1144 ACATGT A^CATGT
1585 ACATGT
BspMI 173 ACCTGC ACCTGC(4/8)
2065 ACCTGC
BsrFI 1251 ACCGGC [GA]^CCGG[CT]
2242 ACCGGC
Bsu36I 268 CCTCAGG CC^T[ACGT]AGG
3345 CCTCAGG
Cfr10I 1251 ACCGGC [GA]^CCGG[CT]
2242 ACCGGC
DraIII 2468 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
3757 CACTTCGTG
DsaI 2662 CCATGG C^C[GA][CT]GG
4074 CCGTGG
Eco81I 268 CCTCAGG CC^T[ACGT]AGG
3345 CCTCAGG
EcoRI 1305 GAATTC G^AATTC
2226 GAATTC
FauI 964 CCCGC CCCGC(4/6)
2111 GCGGG
MamI 976 GATGCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2361 GATCTGTATC
NspI 1144 ACATGT [GA]CATG^[CT]
1585 ACATGT
PflFI 2714 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3284 GACACCGTC
Tth111I 2714 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3284 GACACCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeI NdeII NlaIII
NlaIV PflMI PleI PpuMI PshAI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII