##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AclI AflII AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI AspI AviII BcgI BciVI
BclI BfrI BglII BsiEI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HpaI
KpnI KspI MfeI MluI MunI
NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 343 TCCGGA T^CCGGA
AcsI 2156 AAATTC [GA]^AATT[CT]
AcyI 428 GGCGCC G[GA]^CG[CT]C
AflIII 185 ACACGT A^C[GA][CT]GT
Alw44I 66 GTGCAC G^TGCAC
ApaLI 66 GTGCAC G^TGCAC
ApoI 2156 AAATTC [GA]^AATT[CT]
Asp700I 23 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 428 GGCGCC GGCGC^C
BbrPI 186 CACGTG CAC^GTG
BlpI 449 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 449 GCTCAGC GC^T[ACGT]AGC
BsaAI 186 CACGTG [CT]AC^GT[GA]
BsaBI 1043 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 428 GGCGCC G[GA]^CG[CT]C
BseAI 343 TCCGGA T^CCGGA
BsmBI 634 CGTCTC CGTCTC(1/5)
BspEI 343 TCCGGA T^CCGGA
BstAPI 253 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII 449 GCTCAGC GC^T[ACGT]AGC
DraI 2928 TTTAAA TTT^AAA
EarI 2004 GAAGAG CTCTTC(1/4)
EcoNI 1639 CCTACAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 428 GGCGCC G[GA]^CG[CT]C
HindIII 2068 AAGCTT A^AGCTT
KasI 428 GGCGCC G^GCGCC
Ksp632I 2004 GAAGAG CTCTTC(1/4)
MamI 1043 GATGGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 343 TCCGGA T^CCGGA
NarI 428 GGCGCC GG^CGCC
PaeR7I 1282 CTCGAG C^TCGAG
PmaCI 186 CACGTG CAC^GTG
PmlI 186 CACGTG CAC^GTG
PshAI 380 GACCCGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 1997 AGTACT AGT^ACT
SexAI 2225 ACCTGGT A^CC[AT]GGT
SfoI 428 GGCGCC GGC^GCC
StuI 1915 AGGCCT AGG^CCT
XhoI 1282 CTCGAG C^TCGAG
XmnI 23 GAAGCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 81 CGCG CG^CG
2280 CGCG
AgeI 1954 ACCGGT A^CCGGT
2619 ACCGGT
AocI 903 CCTGAGG CC^T[ACGT]AGG
1077 CCTGAGG
ApaI 778 GGGCCC GGGCC^C
1474 GGGCCC
AvaI 1282 CTCGAG C^[CT]CG[GA]G
1690 CCCGAG
AvrII 369 CCTAGG C^CTAGG
2784 CCTAGG
BamHI 1048 GGATCC G^GATCC
1580 GGATCC
BbsI 377 GAAGAC GAAGAC(2/6)
2535 GTCTTC
BbvCI 658 CCTCAGC CCTCAGC(-5/-2)
2410 CCTCAGC
BglI 1446 GCCCCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2576 GCCAGAGAGGC
BlnI 369 CCTAGG C^CTAGG
2784 CCTAGG
BpuAI 377 GAAGAC GAAGAC(2/6)
2535 GTCTTC
BseRI 1725 CTCCTC GAGGAG(10/8)
168 GAGGAG
BsoBI 1282 CTCGAG C^[CT]CG[GA]G
1690 CCCGAG
BsrDI 937 GCAATG GCAATG(2/0)
2894 GCAATG
BsrFI 1954 ACCGGT [GA]^CCGG[CT]
2619 ACCGGT
BstUI 81 CGCG CG^CG
2280 CGCG
Bsu36I 903 CCTGAGG CC^T[ACGT]AGG
1077 CCTGAGG
Cfr10I 1954 ACCGGT [GA]^CCGG[CT]
2619 ACCGGT
Eco81I 903 CCTGAGG CC^T[ACGT]AGG
1077 CCTGAGG
FauI 104 CCCGC CCCGC(4/6)
2264 CCCGC
HgaI 82 GCGTC GACGC(5/10)
2018 GCGTC
MvnI 81 CGCG CG^CG
2280 CGCG
NcoI 1376 CCATGG C^CATGG
2447 CCATGG
PinAI 1954 ACCGGT A^CCGGT
2619 ACCGGT
SfaNI 1809 GATGC GCATC(5/9)
2396 GCATC
XcmI 886 CCACACACCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1184 CCAGCCAACCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccI AciI AfaI AluI AlwI
AlwNI AspHI AvaII BalI BanI
BanII BbvI BcnI BfaI BmyI
BpmI BsaI BsaJI BsaWI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrI BssKI
BssSI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII