################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## Acc65I AccIII AclI AflII AflIII AhdI AlwNI AocI Aor51HI ApaI AscI Asp718I AspEI AspI AvaI AviII BbrPI BcgI BciVI BfrI BglII BsaBI BsaI BseAI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspHI BspLU11I BsrBI BsrI BssHII BssSI BstAPI Bsu36I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRV EcoT22I FseI FspI HpaI KpnI MamI MluI MroI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SalI SapI ScaI SexAI SfiI SgrAI SmaI SnaBI SpeI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AatII 580 GACGTC GACGT^C AgeI 677 ACCGGT A^CCGGT Alw44I 2603 GTGCAC G^TGCAC ApaLI 2603 GTGCAC G^TGCAC AseI 2068 ATTAAT AT^TAAT AsnI 2068 ATTAAT AT^TAAT AvrII 877 CCTAGG C^CTAGG BamHI 1431 GGATCC G^GATCC BanI 1556 GGCGCC G^G[CT][GA]CC BbeI 1556 GGCGCC GGCGC^C BbvCI 909 GCTGAGG CCTCAGC(-5/-2) BclI 1683 TGATCA T^GATCA BlnI 877 CCTAGG C^CTAGG BsaAI 221 TACGTG [CT]AC^GT[GA] BsaWI 677 ACCGGT [AT]^CCGG[AT] BsrFI 677 ACCGGT [GA]^CCGG[CT] Bst1107I 786 GTATAC GTA^TAC BstEII 1937 GGTCACC G^GT[ACGT]ACC BstPI 1937 GGTCACC G^GT[ACGT]ACC BstXI 1490 CCACGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 786 GTATAC GTA^TAC Cfr10I 677 ACCGGT [GA]^CCGG[CT] DraI 2495 TTTAAA TTT^AAA EcoO65I 1937 GGTCACC G^GT[ACGT]ACC FbaI 1683 TGATCA T^GATCA HincII 1501 GTCAAC GT[CT]^[GA]AC HindII 1501 GTCAAC GT[CT]^[GA]AC HindIII 243 AAGCTT A^AGCTT KasI 1556 GGCGCC G^GCGCC KspI 507 CCGCGG CCGC^GG MfeI 1202 CAATTG C^AATTG MunI 1202 CAATTG C^AATTG NarI 1556 GGCGCC GG^CGCC NcoI 749 CCATGG C^CATGG PflMI 744 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 677 ACCGGT A^CCGGT PshBI 2068 ATTAAT AT^TAAT SacII 507 CCGCGG CCGC^GG SbfI 759 CCTGCAGG CCTGCA^GG SfoI 1556 GGCGCC GGC^GCC SphI 1878 GCATGC GCATG^C Sse8387I 759 CCTGCAGG CCTGCA^GG StuI 769 AGGCCT AGG^CCT SwaI 2494 ATTTAAAT ATTT^AAAT Tsp45I 1938 GTCAC ^GT[GC]AC Van91I 744 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AccI 786 GTATAC GT^[AC][GT]AC 1842 GTCTAC Asp700I 1564 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2366 GAATAGCTTC BalI 467 TGGCCA TGG^CCA 1487 TGGCCA BbsI 1521 GAAGAC GAAGAC(2/6) 1953 GAAGAC BcnI 38 CCGGG CC^[GC]GG 2698 CCGGG BglI 34 GCCTCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 460 GCCTTTTTGGC BlpI 245 GCTTAGC GC^T[ACGT]AGC 1221 GCTAAGC Bpu1102I 245 GCTTAGC GC^T[ACGT]AGC 1221 GCTAAGC BpuAI 1521 GAAGAC GAAGAC(2/6) 1953 GAAGAC BstBI 1073 TTCGAA TT^CGAA 1540 TTCGAA CelII 245 GCTTAGC GC^T[ACGT]AGC 1221 GCTAAGC DrdI 708 GACTCAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 766 GACAGGCCTGTC DsaI 507 CCGCGG C^C[GA][CT]GG 749 CCATGG EaeI 467 TGGCCA [CT]^GGCC[GA] 1487 TGGCCA EarI 557 GAAGAG CTCTTC(1/4) 2523 GAAGAG EcoRI 1543 GAATTC G^AATTC 1797 GAATTC HaeII 1556 GGCGCC [GA]GCGC^[CT] 1725 GGCGCT Ksp632I 557 GAAGAG CTCTTC(1/4) 2523 GAAGAG MluNI 467 TGGCCA TGG^CCA 1487 TGGCCA MscI 467 TGGCCA TGG^CCA 1487 TGGCCA NciI 38 CCGGG CC^[GC]GG 2698 CCGGG NspV 1073 TTCGAA TT^CGAA 1540 TTCGAA PstI 760 CTGCAG CTGCA^G 1857 CTGCAG SacI 1285 GAGCTC GAGCT^C 1849 GAGCTC SfuI 1073 TTCGAA TT^CGAA 1540 TTCGAA SspI 2137 AATATT AAT^ATT 2840 AATATT XmnI 1564 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2366 GAATAGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 96 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI ApoI AspHI AvaII BanII BbvI BfaI BmyI BpmI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XcmI XhoII