##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AhdI AlwNI AocI Aor51HI ApaI
AscI Asp718I AspEI AspI AvaI
AviII BbrPI BcgI BciVI BfrI
BglII BsaBI BsaI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BsrBI BsrI
BssHII BssSI BstAPI Bsu36I ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoRV EcoT22I
FseI FspI HpaI KpnI MamI
MluI MroI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SalI SapI ScaI SexAI SfiI
SgrAI SmaI SnaBI SpeI Tth111I
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 580 GACGTC GACGT^C
AgeI 677 ACCGGT A^CCGGT
Alw44I 2603 GTGCAC G^TGCAC
ApaLI 2603 GTGCAC G^TGCAC
AseI 2068 ATTAAT AT^TAAT
AsnI 2068 ATTAAT AT^TAAT
AvrII 877 CCTAGG C^CTAGG
BamHI 1431 GGATCC G^GATCC
BanI 1556 GGCGCC G^G[CT][GA]CC
BbeI 1556 GGCGCC GGCGC^C
BbvCI 909 GCTGAGG CCTCAGC(-5/-2)
BclI 1683 TGATCA T^GATCA
BlnI 877 CCTAGG C^CTAGG
BsaAI 221 TACGTG [CT]AC^GT[GA]
BsaWI 677 ACCGGT [AT]^CCGG[AT]
BsrFI 677 ACCGGT [GA]^CCGG[CT]
Bst1107I 786 GTATAC GTA^TAC
BstEII 1937 GGTCACC G^GT[ACGT]ACC
BstPI 1937 GGTCACC G^GT[ACGT]ACC
BstXI 1490 CCACGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 786 GTATAC GTA^TAC
Cfr10I 677 ACCGGT [GA]^CCGG[CT]
DraI 2495 TTTAAA TTT^AAA
EcoO65I 1937 GGTCACC G^GT[ACGT]ACC
FbaI 1683 TGATCA T^GATCA
HincII 1501 GTCAAC GT[CT]^[GA]AC
HindII 1501 GTCAAC GT[CT]^[GA]AC
HindIII 243 AAGCTT A^AGCTT
KasI 1556 GGCGCC G^GCGCC
KspI 507 CCGCGG CCGC^GG
MfeI 1202 CAATTG C^AATTG
MunI 1202 CAATTG C^AATTG
NarI 1556 GGCGCC GG^CGCC
NcoI 749 CCATGG C^CATGG
PflMI 744 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 677 ACCGGT A^CCGGT
PshBI 2068 ATTAAT AT^TAAT
SacII 507 CCGCGG CCGC^GG
SbfI 759 CCTGCAGG CCTGCA^GG
SfoI 1556 GGCGCC GGC^GCC
SphI 1878 GCATGC GCATG^C
Sse8387I 759 CCTGCAGG CCTGCA^GG
StuI 769 AGGCCT AGG^CCT
SwaI 2494 ATTTAAAT ATTT^AAAT
Tsp45I 1938 GTCAC ^GT[GC]AC
Van91I 744 CCAGGCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccI 786 GTATAC GT^[AC][GT]AC
1842 GTCTAC
Asp700I 1564 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2366 GAATAGCTTC
BalI 467 TGGCCA TGG^CCA
1487 TGGCCA
BbsI 1521 GAAGAC GAAGAC(2/6)
1953 GAAGAC
BcnI 38 CCGGG CC^[GC]GG
2698 CCGGG
BglI 34 GCCTCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
460 GCCTTTTTGGC
BlpI 245 GCTTAGC GC^T[ACGT]AGC
1221 GCTAAGC
Bpu1102I 245 GCTTAGC GC^T[ACGT]AGC
1221 GCTAAGC
BpuAI 1521 GAAGAC GAAGAC(2/6)
1953 GAAGAC
BstBI 1073 TTCGAA TT^CGAA
1540 TTCGAA
CelII 245 GCTTAGC GC^T[ACGT]AGC
1221 GCTAAGC
DrdI 708 GACTCAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
766 GACAGGCCTGTC
DsaI 507 CCGCGG C^C[GA][CT]GG
749 CCATGG
EaeI 467 TGGCCA [CT]^GGCC[GA]
1487 TGGCCA
EarI 557 GAAGAG CTCTTC(1/4)
2523 GAAGAG
EcoRI 1543 GAATTC G^AATTC
1797 GAATTC
HaeII 1556 GGCGCC [GA]GCGC^[CT]
1725 GGCGCT
Ksp632I 557 GAAGAG CTCTTC(1/4)
2523 GAAGAG
MluNI 467 TGGCCA TGG^CCA
1487 TGGCCA
MscI 467 TGGCCA TGG^CCA
1487 TGGCCA
NciI 38 CCGGG CC^[GC]GG
2698 CCGGG
NspV 1073 TTCGAA TT^CGAA
1540 TTCGAA
PstI 760 CTGCAG CTGCA^G
1857 CTGCAG
SacI 1285 GAGCTC GAGCT^C
1849 GAGCTC
SfuI 1073 TTCGAA TT^CGAA
1540 TTCGAA
SspI 2137 AATATT AAT^ATT
2840 AATATT
XmnI 1564 GAAATGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2366 GAATAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspHI AvaII
BanII BbvI BfaI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspMI BsrDI BsrGI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV NspI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XcmI
XhoII