##################################
# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflIII AgeI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I AviII BbeI BbrPI BcgI
BciVI BclI BglI BlpI Bpu1102I
BsaAI BsaBI BsaHI BseAI BsiEI
BsiWI BspDI BspEI BspLU11I BsrDI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI EagI EclXI Eco47III Eco52I
Eco81I EcoO65I FbaI FseI FspI
Hin1I HpaI KasI KspI MamI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NspV
PacI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I StuI SwaI
Van91I XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 1526 GGTACC G^GTACC
AccII 1978 CGCG CG^CG
AhdI 1324 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 1526 GGTACC G^GTACC
AspEI 1324 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2253 GGATCC G^GATCC
BbvCI 619 CCTCAGC CCTCAGC(-5/-2)
BcnI 301 CCCGG CC^[GC]GG
BsaWI 534 ACCGGA [AT]^CCGG[AT]
BsmI 692 GCATTC GAATGC(1/-1)
Bsp1407I 344 TGTACA T^GTACA
BspHI 1259 TCATGA T^CATGA
BspMI 2933 GCAGGT ACCTGC(4/8)
BsrBI 1166 CCGCTC CCGCTC(-3/-3)
BsrFI 2060 GCCGGC [GA]^CCGG[CT]
BsrGI 344 TGTACA T^GTACA
BstUI 1978 CGCG CG^CG
Cfr10I 2060 GCCGGC [GA]^CCGG[CT]
DraIII 431 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 1324 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 903 GATATC GAT^ATC
EcoT22I 2821 ATGCAT ATGCA^T
FauI 2043 GCGGG CCCGC(4/6)
HaeII 1512 GGCGCT [GA]GCGC^[CT]
HincII 802 GTCAAC GT[CT]^[GA]AC
HindII 802 GTCAAC GT[CT]^[GA]AC
KpnI 1526 GGTACC GGTAC^C
MaeII 1659 ACGT A^CGT
MvnI 1978 CGCG CG^CG
NaeI 2060 GCCGGC GCC^GGC
NciI 301 CCCGG CC^[GC]GG
NgoMIV 2060 GCCGGC G^CCGGC
NsiI 2821 ATGCAT ATGCA^T
RcaI 1259 TCATGA T^CATGA
SapI 870 GCTCTTC GCTCTTC(1/4)
SexAI 2528 ACCAGGT A^CC[AT]GGT
SspBI 344 TGTACA T^GTACA
XbaI 326 TCTAGA T^CTAGA
XcmI 1751 CCAAACCAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AflII 2616 CTTAAG C^TTAAG
2690 CTTAAG
AvrII 720 CCTAGG C^CTAGG
2285 CCTAGG
BanI 1526 GGTACC G^G[CT][GA]CC
2591 GGTGCC
BfrI 2616 CTTAAG C^TTAAG
2690 CTTAAG
BglII 835 AGATCT A^GATCT
1944 AGATCT
BlnI 720 CCTAGG C^CTAGG
2285 CCTAGG
BsmBI 240 CGTCTC CGTCTC(1/5)
366 GAGACG
BssSI 1267 CACGAG CACGAG(-5/-1)
865 CTCGTG
DraI 36 TTTAAA TTT^AAA
1605 TTTAAA
DrdI 643 GACCACCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2577 GACAGCTGAGTC
EcoRI 634 GAATTC G^AATTC
2418 GAATTC
HgaI 368 GACGC GACGC(5/10)
1405 GACGC
NcoI 1112 CCATGG C^CATGG
2358 CCATGG
PaeR7I 1075 CTCGAG C^TCGAG
2476 CTCGAG
PshAI 199 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1984 GACCCAGGTC
XhoI 1075 CTCGAG C^TCGAG
2476 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AspI AvaI
AvaII BalI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV NspI PflFI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XhoII