################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflIII AgeI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AviII BbeI BbrPI BcgI BciVI BclI BglI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BsrDI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI EagI EclXI Eco47III Eco52I Eco81I EcoO65I FbaI FseI FspI Hin1I HpaI KasI KspI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Van91I XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 1526 GGTACC G^GTACC AccII 1978 CGCG CG^CG AhdI 1324 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 1526 GGTACC G^GTACC AspEI 1324 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 2253 GGATCC G^GATCC BbvCI 619 CCTCAGC CCTCAGC(-5/-2) BcnI 301 CCCGG CC^[GC]GG BsaWI 534 ACCGGA [AT]^CCGG[AT] BsmI 692 GCATTC GAATGC(1/-1) Bsp1407I 344 TGTACA T^GTACA BspHI 1259 TCATGA T^CATGA BspMI 2933 GCAGGT ACCTGC(4/8) BsrBI 1166 CCGCTC CCGCTC(-3/-3) BsrFI 2060 GCCGGC [GA]^CCGG[CT] BsrGI 344 TGTACA T^GTACA BstUI 1978 CGCG CG^CG Cfr10I 2060 GCCGGC [GA]^CCGG[CT] DraIII 431 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 1324 GACACACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 903 GATATC GAT^ATC EcoT22I 2821 ATGCAT ATGCA^T FauI 2043 GCGGG CCCGC(4/6) HaeII 1512 GGCGCT [GA]GCGC^[CT] HincII 802 GTCAAC GT[CT]^[GA]AC HindII 802 GTCAAC GT[CT]^[GA]AC KpnI 1526 GGTACC GGTAC^C MaeII 1659 ACGT A^CGT MvnI 1978 CGCG CG^CG NaeI 2060 GCCGGC GCC^GGC NciI 301 CCCGG CC^[GC]GG NgoMIV 2060 GCCGGC G^CCGGC NsiI 2821 ATGCAT ATGCA^T RcaI 1259 TCATGA T^CATGA SapI 870 GCTCTTC GCTCTTC(1/4) SexAI 2528 ACCAGGT A^CC[AT]GGT SspBI 344 TGTACA T^GTACA XbaI 326 TCTAGA T^CTAGA XcmI 1751 CCAAACCAATGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AflII 2616 CTTAAG C^TTAAG 2690 CTTAAG AvrII 720 CCTAGG C^CTAGG 2285 CCTAGG BanI 1526 GGTACC G^G[CT][GA]CC 2591 GGTGCC BfrI 2616 CTTAAG C^TTAAG 2690 CTTAAG BglII 835 AGATCT A^GATCT 1944 AGATCT BlnI 720 CCTAGG C^CTAGG 2285 CCTAGG BsmBI 240 CGTCTC CGTCTC(1/5) 366 GAGACG BssSI 1267 CACGAG CACGAG(-5/-1) 865 CTCGTG DraI 36 TTTAAA TTT^AAA 1605 TTTAAA DrdI 643 GACCACCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2577 GACAGCTGAGTC EcoRI 634 GAATTC G^AATTC 2418 GAATTC HgaI 368 GACGC GACGC(5/10) 1405 GACGC NcoI 1112 CCATGG C^CATGG 2358 CCATGG PaeR7I 1075 CTCGAG C^TCGAG 2476 CTCGAG PshAI 199 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1984 GACCCAGGTC XhoI 1075 CTCGAG C^TCGAG 2476 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AspI AvaI AvaII BalI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflFI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I XhoII