##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI AspEI AvrII BbeI
BbrPI BbvCI BglII BlnI BsaAI
BsaBI BsiWI Bsp1407I BspDI BsrGI
BstBI ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FseI HgaI HpaI
KasI KspI MamI MluI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PmaCI
PmeI PmlI PshAI PshBI PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 1813 GACGTC GACGT^C
AclI 4248 AACGTT AA^CGTT
AcyI 1813 GACGTC G[GA]^CG[CT]C
AflII 3349 CTTAAG C^TTAAG
AgeI 334 ACCGGT A^CCGGT
AocI 61 CCTCAGG CC^T[ACGT]AGG
AviII 272 TGCGCA TGC^GCA
BcgI 2269 GCAACATGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 430 GGATAC GTATCC(6/5)
BclI 1236 TGATCA T^GATCA
BfrI 3349 CTTAAG C^TTAAG
BglI 3681 GCCACTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1779 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1779 GCTCAGC GC^T[ACGT]AGC
BsaHI 1813 GACGTC G[GA]^CG[CT]C
BsiEI 291 CGACCG CG[GA][CT]^CG
BsmBI 4498 CGTCTC CGTCTC(1/5)
BspHI 1689 TCATGA T^CATGA
BssHII 2170 GCGCGC G^CGCGC
BssSI 4066 CTCGTG CACGAG(-5/-1)
Bst1107I 3462 GTATAC GTA^TAC
BstXI 3501 CCAAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 3462 GTATAC GTA^TAC
Bsu36I 61 CCTCAGG CC^T[ACGT]AGG
CelII 1779 GCTCAGC GC^T[ACGT]AGC
Eco81I 61 CCTCAGG CC^T[ACGT]AGG
EcoRI 296 GAATTC G^AATTC
FauI 712 GCGGG CCCGC(4/6)
FbaI 1236 TGATCA T^GATCA
FspI 272 TGCGCA TGC^GCA
Hin1I 1813 GACGTC G[GA]^CG[CT]C
HincII 1855 GTCAAC GT[CT]^[GA]AC
HindII 1855 GTCAAC GT[CT]^[GA]AC
HindIII 3597 AAGCTT A^AGCTT
NheI 233 GCTAGC G^CTAGC
PinAI 334 ACCGGT A^CCGGT
Psp1406I 4248 AACGTT AA^CGTT
RcaI 1689 TCATGA T^CATGA
SmaI 2208 CCCGGG CCC^GGG
SspI 3071 AATATT AAT^ATT
StuI 2877 AGGCCT AGG^CCT
XbaI 4459 TCTAGA T^CTAGA
XmaCI 2208 CCCGGG C^CCGGG
XmaI 2208 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 2365 GTCTAC GT^[AC][GT]AC
3462 GTATAC
AccII 112 CGCG CG^CG
2171 CGCG
AccIII 2723 TCCGGA T^CCGGA
3781 TCCGGA
AflIII 1435 ACATGT A^C[GA][CT]GT
2272 ACATGT
ApaI 470 GGGCCC GGGCC^C
3688 GGGCCC
AspI 84 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1475 GACTACGTC
BseAI 2723 TCCGGA T^CCGGA
3781 TCCGGA
BsgI 627 CTGCAC GTGCAG(16/14)
2010 CTGCAC
BsmI 1791 GAATGC GAATGC(1/-1)
1109 GCATTC
BspEI 2723 TCCGGA T^CCGGA
3781 TCCGGA
BspLU11I 1435 ACATGT A^CATGT
2272 ACATGT
BsrFI 334 ACCGGT [GA]^CCGG[CT]
1413 ACCGGC
BstAPI 1598 GCACTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1973 GCATCTACTGC
BstEII 89 GGTCACC G^GT[ACGT]ACC
1370 GGTAACC
BstPI 89 GGTCACC G^GT[ACGT]ACC
1370 GGTAACC
BstUI 112 CGCG CG^CG
2171 CGCG
Cfr10I 334 ACCGGT [GA]^CCGG[CT]
1413 ACCGGC
DraIII 1599 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
3733 CACAAGGTG
EcoO65I 89 GGTCACC G^GT[ACGT]ACC
1370 GGTAACC
HaeII 726 AGCGCC [GA]GCGC^[CT]
1307 AGCGCC
MfeI 3323 CAATTG C^AATTG
3890 CAATTG
MroI 2723 TCCGGA T^CCGGA
3781 TCCGGA
MunI 3323 CAATTG C^AATTG
3890 CAATTG
MvnI 112 CGCG CG^CG
2171 CGCG
PflFI 84 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1475 GACTACGTC
PvuII 1085 CAGCTG CAG^CTG
2202 CAGCTG
SapI 2819 GAAGAGC GCTCTTC(1/4)
8 GCTCTTC
TaqI 3142 TCGA T^CGA
4223 TCGA
Tth111I 84 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1475 GACTACGTC
TthHB8I 3142 TCGA T^CGA
4223 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I Asp718I
AspHI AvaI AvaII BalI BamHI
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI KpnI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI RsaI SacI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XcmI XhoII XmnI