################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI AocI Aor51HI AscI AspEI AspI AviII AvrII BbeI BcgI BfrI BglI BlnI BsaBI BsaI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FauI FseI FspI HindIII KasI KspI MamI MluI MroI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI Psp1406I PvuI RsrII SacII SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### Acc65I 3143 GGTACC G^GTACC AccII 39 CGCG CG^CG AcyI 3152 GACGCC G[GA]^CG[CT]C AflIII 2061 ACATGT A^C[GA][CT]GT Alw44I 2077 GTGCAC G^TGCAC ApaI 675 GGGCCC GGGCC^C ApaLI 2077 GTGCAC G^TGCAC AseI 52 ATTAAT AT^TAAT AsnI 52 ATTAAT AT^TAAT Asp718I 3143 GGTACC G^GTACC AvaI 1473 CCCGAG C^[CT]CG[GA]G BalI 614 TGGCCA TGG^CCA BamHI 294 GGATCC G^GATCC BanI 3143 GGTACC G^G[CT][GA]CC BbrPI 3197 CACGTG CAC^GTG BbvCI 2005 CCTCAGC CCTCAGC(-5/-2) BciVI 668 GTATCC GTATCC(6/5) BcnI 14 CCGGG CC^[GC]GG BlpI 1102 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1102 GCTGAGC GC^T[ACGT]AGC BsaAI 3197 CACGTG [CT]AC^GT[GA] BsaHI 3152 GACGCC G[GA]^CG[CT]C BsaWI 2033 ACCGGA [AT]^CCGG[AT] BsgI 1391 GTGCAG GTGCAG(16/14) BsoBI 1473 CCCGAG C^[CT]CG[GA]G BspLU11I 2061 ACATGT A^CATGT BsrFI 2917 GCCGGT [GA]^CCGG[CT] BstUI 39 CGCG CG^CG CelII 1102 GCTGAGC GC^T[ACGT]AGC Cfr10I 2917 GCCGGT [GA]^CCGG[CT] DsaI 1686 CCACGG C^C[GA][CT]GG EaeI 614 TGGCCA [CT]^GGCC[GA] EcoT14I 1600 CCTTGG C^C[AT][AT]GG HaeII 3180 GGCGCT [GA]GCGC^[CT] HgaI 3152 GACGC GACGC(5/10) Hin1I 3152 GACGCC G[GA]^CG[CT]C HpaI 2182 GTTAAC GTT^AAC KpnI 3143 GGTACC GGTAC^C MfeI 775 CAATTG C^AATTG MluNI 614 TGGCCA TGG^CCA MscI 614 TGGCCA TGG^CCA MslI 2590 CATTGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 440 CAGCTG C[AC]G^C[GT]G MunI 775 CAATTG C^AATTG MvnI 39 CGCG CG^CG NciI 14 CCGGG CC^[GC]GG NdeI 2177 CATATG CA^TATG NspI 2061 ACATGT [GA]CATG^[CT] PflMI 77 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 3197 CACGTG CAC^GTG PmlI 3197 CACGTG CAC^GTG PpuMI 571 AGGTCCT [GA]G^G[AT]CC[CT] PshBI 52 ATTAAT AT^TAAT PvuII 440 CAGCTG CAG^CTG SalI 852 GTCGAC G^TCGAC ScaI 1150 AGTACT AGT^ACT SexAI 568 ACCAGGT A^CC[AT]GGT SpeI 2360 ACTAGT A^CTAGT SspI 2379 AATATT AAT^ATT StyI 1600 CCTTGG C^C[AT][AT]GG Van91I 77 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 2697 CCACATAATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AccI 852 GTCGAC GT^[AC][GT]AC 2725 GTAGAC BglII 804 AGATCT A^GATCT 1648 AGATCT BsmI 2599 GAATGC GAATGC(1/-1) 873 GCATTC CfoI 38 GCGC GCG^C 3181 GCGC DraI 1753 TTTAAA TTT^AAA 2335 TTTAAA DrdI 1138 GACACAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1254 GACATCTAAGTC HhaI 38 GCGC GCG^C 3181 GCGC HinP1I 38 GCGC G^CGC 3181 GCGC HincII 852 GTCGAC GT[CT]^[GA]AC 2182 GTTAAC HindII 852 GTCGAC GT[CT]^[GA]AC 2182 GTTAAC MaeII 174 ACGT A^CGT 3198 ACGT SacI 697 GAGCTC GAGCT^C 2930 GAGCTC SapI 520 GCTCTTC GCTCTTC(1/4) 2838 GCTCTTC SmlI 183 CTCAAG C^T[CT][GA]AG 2925 CTCAAG TaqI 246 TCGA T^CGA 853 TCGA Tsp45I 1136 GTGAC ^GT[GC]AC 2286 GTGAC TthHB8I 246 TCGA T^CGA 853 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BanII BbsI BbvI BclI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspHI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRI EcoRII FbaI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV PleI PstI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TfiI Tru9I TseI Tsp509I TspRI XhoII XmnI