##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI AocI Aor51HI AscI AspEI
AspI AviII AvrII BbeI BcgI
BfrI BglI BlnI BsaBI BsaI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRV EcoT22I FauI FseI
FspI HindIII KasI KspI MamI
MluI MroI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI Psp1406I PvuI RsrII
SacII SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
Acc65I 3143 GGTACC G^GTACC
AccII 39 CGCG CG^CG
AcyI 3152 GACGCC G[GA]^CG[CT]C
AflIII 2061 ACATGT A^C[GA][CT]GT
Alw44I 2077 GTGCAC G^TGCAC
ApaI 675 GGGCCC GGGCC^C
ApaLI 2077 GTGCAC G^TGCAC
AseI 52 ATTAAT AT^TAAT
AsnI 52 ATTAAT AT^TAAT
Asp718I 3143 GGTACC G^GTACC
AvaI 1473 CCCGAG C^[CT]CG[GA]G
BalI 614 TGGCCA TGG^CCA
BamHI 294 GGATCC G^GATCC
BanI 3143 GGTACC G^G[CT][GA]CC
BbrPI 3197 CACGTG CAC^GTG
BbvCI 2005 CCTCAGC CCTCAGC(-5/-2)
BciVI 668 GTATCC GTATCC(6/5)
BcnI 14 CCGGG CC^[GC]GG
BlpI 1102 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1102 GCTGAGC GC^T[ACGT]AGC
BsaAI 3197 CACGTG [CT]AC^GT[GA]
BsaHI 3152 GACGCC G[GA]^CG[CT]C
BsaWI 2033 ACCGGA [AT]^CCGG[AT]
BsgI 1391 GTGCAG GTGCAG(16/14)
BsoBI 1473 CCCGAG C^[CT]CG[GA]G
BspLU11I 2061 ACATGT A^CATGT
BsrFI 2917 GCCGGT [GA]^CCGG[CT]
BstUI 39 CGCG CG^CG
CelII 1102 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2917 GCCGGT [GA]^CCGG[CT]
DsaI 1686 CCACGG C^C[GA][CT]GG
EaeI 614 TGGCCA [CT]^GGCC[GA]
EcoT14I 1600 CCTTGG C^C[AT][AT]GG
HaeII 3180 GGCGCT [GA]GCGC^[CT]
HgaI 3152 GACGC GACGC(5/10)
Hin1I 3152 GACGCC G[GA]^CG[CT]C
HpaI 2182 GTTAAC GTT^AAC
KpnI 3143 GGTACC GGTAC^C
MfeI 775 CAATTG C^AATTG
MluNI 614 TGGCCA TGG^CCA
MscI 614 TGGCCA TGG^CCA
MslI 2590 CATTGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 440 CAGCTG C[AC]G^C[GT]G
MunI 775 CAATTG C^AATTG
MvnI 39 CGCG CG^CG
NciI 14 CCGGG CC^[GC]GG
NdeI 2177 CATATG CA^TATG
NspI 2061 ACATGT [GA]CATG^[CT]
PflMI 77 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3197 CACGTG CAC^GTG
PmlI 3197 CACGTG CAC^GTG
PpuMI 571 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 52 ATTAAT AT^TAAT
PvuII 440 CAGCTG CAG^CTG
SalI 852 GTCGAC G^TCGAC
ScaI 1150 AGTACT AGT^ACT
SexAI 568 ACCAGGT A^CC[AT]GGT
SpeI 2360 ACTAGT A^CTAGT
SspI 2379 AATATT AAT^ATT
StyI 1600 CCTTGG C^C[AT][AT]GG
Van91I 77 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2697 CCACATAATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 852 GTCGAC GT^[AC][GT]AC
2725 GTAGAC
BglII 804 AGATCT A^GATCT
1648 AGATCT
BsmI 2599 GAATGC GAATGC(1/-1)
873 GCATTC
CfoI 38 GCGC GCG^C
3181 GCGC
DraI 1753 TTTAAA TTT^AAA
2335 TTTAAA
DrdI 1138 GACACAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1254 GACATCTAAGTC
HhaI 38 GCGC GCG^C
3181 GCGC
HinP1I 38 GCGC G^CGC
3181 GCGC
HincII 852 GTCGAC GT[CT]^[GA]AC
2182 GTTAAC
HindII 852 GTCGAC GT[CT]^[GA]AC
2182 GTTAAC
MaeII 174 ACGT A^CGT
3198 ACGT
SacI 697 GAGCTC GAGCT^C
2930 GAGCTC
SapI 520 GCTCTTC GCTCTTC(1/4)
2838 GCTCTTC
SmlI 183 CTCAAG C^T[CT][GA]AG
2925 CTCAAG
TaqI 246 TCGA T^CGA
853 TCGA
Tsp45I 1136 GTGAC ^GT[GC]AC
2286 GTGAC
TthHB8I 246 TCGA T^CGA
853 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BanII BbsI BbvI BclI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRI EcoRII FbaI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PstI RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TfiI
Tru9I TseI Tsp509I TspRI XhoII
XmnI