##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII AccIII AgeI AocI AscI
Asp700I AvaI AviII BbrPI BbvCI
BcgI BciVI BglII BsaAI BseAI
BsiWI BsoBI BspDI BspEI BsrDI
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraIII DrdI Eco81I
EcoO65I EcoRI FseI FspI MluI
MroI NaeI NdeI NgoMIV NheI
NruI NspV PaeR7I PflMI PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SalI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI Sse8387I
SwaI Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 3506 GGTACC G^GTACC
AccI 2007 GTCTAC GT^[AC][GT]AC
AclI 957 AACGTT AA^CGTT
AhdI 197 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 3778 ATTAAT AT^TAAT
AsnI 3778 ATTAAT AT^TAAT
Asp718I 3506 GGTACC G^GTACC
AspEI 197 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2402 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2386 CCTAGG C^CTAGG
BalI 2995 TGGCCA TGG^CCA
BamHI 1102 GGATCC G^GATCC
BbeI 3113 GGCGCC GGCGC^C
BglI 1826 GCCTTTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2386 CCTAGG C^CTAGG
BlpI 1861 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1861 GCTCAGC GC^T[ACGT]AGC
BsaBI 588 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3420 ACCGGA [AT]^CCGG[AT]
BsmBI 492 GAGACG CGTCTC(1/5)
BspHI 99 TCATGA T^CATGA
BstXI 473 CCAAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1861 GCTCAGC GC^T[ACGT]AGC
EagI 1471 CGGCCG C^GGCCG
Eam1105I 197 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1471 CGGCCG C^GGCCG
Eco52I 1471 CGGCCG C^GGCCG
EcoNI 921 CCTCTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1158 GATATC GAT^ATC
EcoT22I 774 ATGCAT ATGCA^T
HpaI 3835 GTTAAC GTT^AAC
KasI 3113 GGCGCC G^GCGCC
KpnI 3506 GGTACC GGTAC^C
KspI 3349 CCGCGG CCGC^GG
MaeII 958 ACGT A^CGT
MamI 588 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 3925 CAATTG C^AATTG
MluNI 2995 TGGCCA TGG^CCA
MscI 2995 TGGCCA TGG^CCA
MunI 3925 CAATTG C^AATTG
NarI 3113 GGCGCC GG^CGCC
NotI 1470 GCGGCCGC GC^GGCCGC
NsiI 774 ATGCAT ATGCA^T
PacI 3775 TTAATTAA TTAAT^TAA
PflFI 2402 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3778 ATTAAT AT^TAAT
Psp1406I 957 AACGTT AA^CGTT
PvuII 1682 CAGCTG CAG^CTG
RcaI 99 TCATGA T^CATGA
SacI 2547 GAGCTC GAGCT^C
SacII 3349 CCGCGG CCGC^GG
ScaI 1112 AGTACT AGT^ACT
SfoI 3113 GGCGCC GGC^GCC
SpeI 730 ACTAGT A^CTAGT
SphI 2463 GCATGC GCATG^C
StuI 3427 AGGCCT AGG^CCT
Tth111I 2402 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 4031 CCAGGGAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AflII 1728 CTTAAG C^TTAAG
4146 CTTAAG
AflIII 1343 ACATGT A^C[GA][CT]GT
3580 ACATGT
Alw44I 151 GTGCAC G^TGCAC
3666 GTGCAC
Aor51HI 1301 AGCGCT AGC^GCT
1480 AGCGCT
ApaI 3037 GGGCCC GGGCC^C
3066 GGGCCC
ApaLI 151 GTGCAC G^TGCAC
3666 GTGCAC
BclI 435 TGATCA T^GATCA
1911 TGATCA
BcnI 845 CCCGG CC^[GC]GG
3369 CCGGG
BfrI 1728 CTTAAG C^TTAAG
4146 CTTAAG
BsiEI 1471 CGGCCG CG[GA][CT]^CG
3366 CGACCG
BsmI 1139 GCATTC GAATGC(1/-1)
2122 GCATTC
Bsp1407I 3911 TGTACA T^GTACA
4154 TGTACA
BspLU11I 1343 ACATGT A^CATGT
3580 ACATGT
BsrGI 3911 TGTACA T^GTACA
4154 TGTACA
BstAPI 280 GCAAAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
767 GCAAAATATGC
DraI 3738 TTTAAA TTT^AAA
3938 TTTAAA
Eco47III 1301 AGCGCT AGC^GCT
1480 AGCGCT
FbaI 435 TGATCA T^GATCA
1911 TGATCA
HindIII 360 AAGCTT A^AGCTT
4149 AAGCTT
MslI 516 CATAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
577 CACAAAGGTG
NciI 845 CCCGG CC^[GC]GG
3369 CCGGG
NcoI 2984 CCATGG C^CATGG
3127 CCATGG
SapI 2544 GAAGAGC GCTCTTC(1/4)
4126 GCTCTTC
SspBI 3911 TGTACA T^GTACA
4154 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BanI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII