################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII AccIII AgeI AocI AscI Asp700I AvaI AviII BbrPI BbvCI BcgI BciVI BglII BsaAI BseAI BsiWI BsoBI BspDI BspEI BsrDI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII DrdI Eco81I EcoO65I EcoRI FseI FspI MluI MroI NaeI NdeI NgoMIV NheI NruI NspV PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PvuI RsrII SalI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI Sse8387I SwaI Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 3506 GGTACC G^GTACC AccI 2007 GTCTAC GT^[AC][GT]AC AclI 957 AACGTT AA^CGTT AhdI 197 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 3778 ATTAAT AT^TAAT AsnI 3778 ATTAAT AT^TAAT Asp718I 3506 GGTACC G^GTACC AspEI 197 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2402 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 2386 CCTAGG C^CTAGG BalI 2995 TGGCCA TGG^CCA BamHI 1102 GGATCC G^GATCC BbeI 3113 GGCGCC GGCGC^C BglI 1826 GCCTTTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 2386 CCTAGG C^CTAGG BlpI 1861 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1861 GCTCAGC GC^T[ACGT]AGC BsaBI 588 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 3420 ACCGGA [AT]^CCGG[AT] BsmBI 492 GAGACG CGTCTC(1/5) BspHI 99 TCATGA T^CATGA BstXI 473 CCAAAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1861 GCTCAGC GC^T[ACGT]AGC EagI 1471 CGGCCG C^GGCCG Eam1105I 197 GACAGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 1471 CGGCCG C^GGCCG Eco52I 1471 CGGCCG C^GGCCG EcoNI 921 CCTCTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1158 GATATC GAT^ATC EcoT22I 774 ATGCAT ATGCA^T HpaI 3835 GTTAAC GTT^AAC KasI 3113 GGCGCC G^GCGCC KpnI 3506 GGTACC GGTAC^C KspI 3349 CCGCGG CCGC^GG MaeII 958 ACGT A^CGT MamI 588 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3925 CAATTG C^AATTG MluNI 2995 TGGCCA TGG^CCA MscI 2995 TGGCCA TGG^CCA MunI 3925 CAATTG C^AATTG NarI 3113 GGCGCC GG^CGCC NotI 1470 GCGGCCGC GC^GGCCGC NsiI 774 ATGCAT ATGCA^T PacI 3775 TTAATTAA TTAAT^TAA PflFI 2402 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 3778 ATTAAT AT^TAAT Psp1406I 957 AACGTT AA^CGTT PvuII 1682 CAGCTG CAG^CTG RcaI 99 TCATGA T^CATGA SacI 2547 GAGCTC GAGCT^C SacII 3349 CCGCGG CCGC^GG ScaI 1112 AGTACT AGT^ACT SfoI 3113 GGCGCC GGC^GCC SpeI 730 ACTAGT A^CTAGT SphI 2463 GCATGC GCATG^C StuI 3427 AGGCCT AGG^CCT Tth111I 2402 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 4031 CCAGGGAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AflII 1728 CTTAAG C^TTAAG 4146 CTTAAG AflIII 1343 ACATGT A^C[GA][CT]GT 3580 ACATGT Alw44I 151 GTGCAC G^TGCAC 3666 GTGCAC Aor51HI 1301 AGCGCT AGC^GCT 1480 AGCGCT ApaI 3037 GGGCCC GGGCC^C 3066 GGGCCC ApaLI 151 GTGCAC G^TGCAC 3666 GTGCAC BclI 435 TGATCA T^GATCA 1911 TGATCA BcnI 845 CCCGG CC^[GC]GG 3369 CCGGG BfrI 1728 CTTAAG C^TTAAG 4146 CTTAAG BsiEI 1471 CGGCCG CG[GA][CT]^CG 3366 CGACCG BsmI 1139 GCATTC GAATGC(1/-1) 2122 GCATTC Bsp1407I 3911 TGTACA T^GTACA 4154 TGTACA BspLU11I 1343 ACATGT A^CATGT 3580 ACATGT BsrGI 3911 TGTACA T^GTACA 4154 TGTACA BstAPI 280 GCAAAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 767 GCAAAATATGC DraI 3738 TTTAAA TTT^AAA 3938 TTTAAA Eco47III 1301 AGCGCT AGC^GCT 1480 AGCGCT FbaI 435 TGATCA T^GATCA 1911 TGATCA HindIII 360 AAGCTT A^AGCTT 4149 AAGCTT MslI 516 CATAGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 577 CACAAAGGTG NciI 845 CCCGG CC^[GC]GG 3369 CCGGG NcoI 2984 CCATGG C^CATGG 3127 CCATGG SapI 2544 GAAGAGC GCTCTTC(1/4) 4126 GCTCTTC SspBI 3911 TGTACA T^GTACA 4154 TGTACA ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII