##################################
# NO CUT ENZYMES ( 78 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AocI Aor51HI ApaI AscI Asp718I
AspI AviII AvrII BamHI BbeI
BbvCI BcgI BglII BlnI BsaHI
BseAI BsiWI BsmBI BsmI BspEI
BssHII Bst1107I BstBI BstZ17I Bsu36I
CpoI DraII DraIII DrdI Eco47III
Eco81I EcoNI EcoO109I EcoRV FseI
FspI HgaI Hin1I HpaI KasI
KpnI MluI MroI NarI NcoI
NheI NruI NspV PacI PflFI
PmeI PpuMI Psp1406I PvuI RsrII
SacI SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SwaI Tth111I
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 75 enzymes) #
###################################
AccII 13 CGCG CG^CG
AflII 2271 CTTAAG C^TTAAG
AflIII 3219 ACATGT A^C[GA][CT]GT
AgeI 1427 ACCGGT A^CCGGT
AhdI 173 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 803 GTGCAC G^TGCAC
ApaLI 803 GTGCAC G^TGCAC
AspEI 173 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 803 GTGCAC G[AT]GC[AT]^C
BalI 2600 TGGCCA TGG^CCA
BanI 1430 GGTGCC G^G[CT][GA]CC
BanII 1918 GAGCCC G[GA]GC[CT]^C
BbrPI 1913 CACGTG CAC^GTG
BciVI 249 GGATAC GTATCC(6/5)
BcnI 20 CCGGG CC^[GC]GG
BfrI 2271 CTTAAG C^TTAAG
BglI 19 GCCGGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 605 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 605 GCTCAGC GC^T[ACGT]AGC
BsaAI 1913 CACGTG [CT]AC^GT[GA]
BsaBI 697 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 871 GAGACC GGTCTC(1/5)
BsaWI 1427 ACCGGT [AT]^CCGG[AT]
BsgI 67 CTGCAC GTGCAG(16/14)
BsiHKAI 803 GTGCAC G[AT]GC[AT]^C
Bsp1407I 3458 TGTACA T^GTACA
BspDI 1122 ATCGAT AT^CGAT
BspHI 2463 TCATGA T^CATGA
BspLU11I 3219 ACATGT A^CATGT
BsrBI 3 GAGCGG CCGCTC(-3/-3)
BsrGI 3458 TGTACA T^GTACA
BssSI 517 CACGAG CACGAG(-5/-1)
BstEII 190 GGTCACC G^GT[ACGT]ACC
BstPI 190 GGTCACC G^GT[ACGT]ACC
BstUI 13 CGCG CG^CG
BstYI 2378 GGATCT [GA]^GATC[CT]
CelII 605 GCTCAGC GC^T[ACGT]AGC
CfoI 3074 GCGC GCG^C
ClaI 1122 ATCGAT AT^CGAT
DsaI 12 CCGCGG C^C[GA][CT]GG
EagI 78 CGGCCG C^GGCCG
Eam1105I 173 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 78 CGGCCG C^GGCCG
Eco52I 78 CGGCCG C^GGCCG
EcoO65I 190 GGTCACC G^GT[ACGT]ACC
HaeII 3073 AGCGCC [GA]GCGC^[CT]
HhaI 3074 GCGC GCG^C
HinP1I 3074 GCGC G^CGC
HincII 3328 GTCAAC GT[CT]^[GA]AC
HindII 3328 GTCAAC GT[CT]^[GA]AC
HindIII 1340 AAGCTT A^AGCTT
KspI 12 CCGCGG CCGC^GG
MamI 697 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2355 CAATTG C^AATTG
MflI 2378 GGATCT [GA]^GATC[CT]
MluNI 2600 TGGCCA TGG^CCA
MscI 2600 TGGCCA TGG^CCA
MunI 2355 CAATTG C^AATTG
MvnI 13 CGCG CG^CG
NaeI 1193 GCCGGC GCC^GGC
NciI 20 CCGGG CC^[GC]GG
NgoMIV 1193 GCCGGC G^CCGGC
NotI 77 GCGGCCGC GC^GGCCGC
PaeR7I 840 CTCGAG C^TCGAG
PinAI 1427 ACCGGT A^CCGGT
PmaCI 1913 CACGTG CAC^GTG
PmlI 1913 CACGTG CAC^GTG
PshAI 3383 GACTAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2463 TCATGA T^CATGA
SacII 12 CCGCGG CCGC^GG
SpeI 3361 ACTAGT A^CTAGT
SspBI 3458 TGTACA T^GTACA
StuI 2664 AGGCCT AGG^CCT
XhoI 840 CTCGAG C^TCGAG
XhoII 2378 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 1184 GTAGAC GT^[AC][GT]AC
3272 GTCTAC
Asp700I 753 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1572 GAAATTATTC
AvaI 840 CTCGAG C^[CT]CG[GA]G
2446 CCCGAG
BclI 1068 TGATCA T^GATCA
3411 TGATCA
BpmI 340 CTGGAG CTGGAG(16/14)
1888 CTGGAG
BseRI 57 GAGGAG GAGGAG(10/8)
2368 GAGGAG
BsiEI 6 CGGTCG CG[GA][CT]^CG
78 CGGCCG
BsoBI 840 CTCGAG C^[CT]CG[GA]G
2446 CCCGAG
BspMI 1903 ACCTGC ACCTGC(4/8)
275 GCAGGT
BsrDI 2959 CATTGC GCAATG(2/0)
431 GCAATG
BsrFI 1193 GCCGGC [GA]^CCGG[CT]
1427 ACCGGT
BstAPI 796 GCAGATTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1230 GCAGCAACTGC
BstXI 1619 CCAAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3359 CCACTAGTGTGG
Cfr10I 1193 GCCGGC [GA]^CCGG[CT]
1427 ACCGGT
EaeI 78 CGGCCG [CT]^GGCC[GA]
2600 TGGCCA
EcoRI 527 GAATTC G^AATTC
2125 GAATTC
EcoT22I 2725 ATGCAT ATGCA^T
2843 ATGCAT
FbaI 1068 TGATCA T^GATCA
3411 TGATCA
MaeII 1288 ACGT A^CGT
1914 ACGT
NsiI 2725 ATGCAT ATGCA^T
2843 ATGCAT
PflMI 239 CCAATATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
265 CCATCTGGTGG
PstI 1307 CTGCAG CTGCA^G
3430 CTGCAG
SapI 1346 GAAGAGC GCTCTTC(1/4)
629 GCTCTTC
Van91I 239 CCAATATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
265 CCATCTGGTGG
XmnI 753 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1572 GAAATTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AvaII
BbsI BbvI BfaI BmyI BpuAI
BsaJI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeI
NdeII NlaIII NlaIV NspI PleI
PshBI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI