################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AocI Aor51HI ApaI AscI Asp718I AspI AviII AvrII BamHI BbeI BbvCI BcgI BglII BlnI BsaHI BseAI BsiWI BsmBI BsmI BspEI BssHII Bst1107I BstBI BstZ17I Bsu36I CpoI DraII DraIII DrdI Eco47III Eco81I EcoNI EcoO109I EcoRV FseI FspI HgaI Hin1I HpaI KasI KpnI MluI MroI NarI NcoI NheI NruI NspV PacI PflFI PmeI PpuMI Psp1406I PvuI RsrII SacI SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SwaI Tth111I XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 75 enzymes) # ################################### AccII 13 CGCG CG^CG AflII 2271 CTTAAG C^TTAAG AflIII 3219 ACATGT A^C[GA][CT]GT AgeI 1427 ACCGGT A^CCGGT AhdI 173 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 803 GTGCAC G^TGCAC ApaLI 803 GTGCAC G^TGCAC AspEI 173 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 803 GTGCAC G[AT]GC[AT]^C BalI 2600 TGGCCA TGG^CCA BanI 1430 GGTGCC G^G[CT][GA]CC BanII 1918 GAGCCC G[GA]GC[CT]^C BbrPI 1913 CACGTG CAC^GTG BciVI 249 GGATAC GTATCC(6/5) BcnI 20 CCGGG CC^[GC]GG BfrI 2271 CTTAAG C^TTAAG BglI 19 GCCGGGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 605 GCTCAGC GC^T[ACGT]AGC Bpu1102I 605 GCTCAGC GC^T[ACGT]AGC BsaAI 1913 CACGTG [CT]AC^GT[GA] BsaBI 697 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 871 GAGACC GGTCTC(1/5) BsaWI 1427 ACCGGT [AT]^CCGG[AT] BsgI 67 CTGCAC GTGCAG(16/14) BsiHKAI 803 GTGCAC G[AT]GC[AT]^C Bsp1407I 3458 TGTACA T^GTACA BspDI 1122 ATCGAT AT^CGAT BspHI 2463 TCATGA T^CATGA BspLU11I 3219 ACATGT A^CATGT BsrBI 3 GAGCGG CCGCTC(-3/-3) BsrGI 3458 TGTACA T^GTACA BssSI 517 CACGAG CACGAG(-5/-1) BstEII 190 GGTCACC G^GT[ACGT]ACC BstPI 190 GGTCACC G^GT[ACGT]ACC BstUI 13 CGCG CG^CG BstYI 2378 GGATCT [GA]^GATC[CT] CelII 605 GCTCAGC GC^T[ACGT]AGC CfoI 3074 GCGC GCG^C ClaI 1122 ATCGAT AT^CGAT DsaI 12 CCGCGG C^C[GA][CT]GG EagI 78 CGGCCG C^GGCCG Eam1105I 173 GACCGAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 78 CGGCCG C^GGCCG Eco52I 78 CGGCCG C^GGCCG EcoO65I 190 GGTCACC G^GT[ACGT]ACC HaeII 3073 AGCGCC [GA]GCGC^[CT] HhaI 3074 GCGC GCG^C HinP1I 3074 GCGC G^CGC HincII 3328 GTCAAC GT[CT]^[GA]AC HindII 3328 GTCAAC GT[CT]^[GA]AC HindIII 1340 AAGCTT A^AGCTT KspI 12 CCGCGG CCGC^GG MamI 697 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2355 CAATTG C^AATTG MflI 2378 GGATCT [GA]^GATC[CT] MluNI 2600 TGGCCA TGG^CCA MscI 2600 TGGCCA TGG^CCA MunI 2355 CAATTG C^AATTG MvnI 13 CGCG CG^CG NaeI 1193 GCCGGC GCC^GGC NciI 20 CCGGG CC^[GC]GG NgoMIV 1193 GCCGGC G^CCGGC NotI 77 GCGGCCGC GC^GGCCGC PaeR7I 840 CTCGAG C^TCGAG PinAI 1427 ACCGGT A^CCGGT PmaCI 1913 CACGTG CAC^GTG PmlI 1913 CACGTG CAC^GTG PshAI 3383 GACTAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 2463 TCATGA T^CATGA SacII 12 CCGCGG CCGC^GG SpeI 3361 ACTAGT A^CTAGT SspBI 3458 TGTACA T^GTACA StuI 2664 AGGCCT AGG^CCT XhoI 840 CTCGAG C^TCGAG XhoII 2378 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 1184 GTAGAC GT^[AC][GT]AC 3272 GTCTAC Asp700I 753 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1572 GAAATTATTC AvaI 840 CTCGAG C^[CT]CG[GA]G 2446 CCCGAG BclI 1068 TGATCA T^GATCA 3411 TGATCA BpmI 340 CTGGAG CTGGAG(16/14) 1888 CTGGAG BseRI 57 GAGGAG GAGGAG(10/8) 2368 GAGGAG BsiEI 6 CGGTCG CG[GA][CT]^CG 78 CGGCCG BsoBI 840 CTCGAG C^[CT]CG[GA]G 2446 CCCGAG BspMI 1903 ACCTGC ACCTGC(4/8) 275 GCAGGT BsrDI 2959 CATTGC GCAATG(2/0) 431 GCAATG BsrFI 1193 GCCGGC [GA]^CCGG[CT] 1427 ACCGGT BstAPI 796 GCAGATTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1230 GCAGCAACTGC BstXI 1619 CCAAGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3359 CCACTAGTGTGG Cfr10I 1193 GCCGGC [GA]^CCGG[CT] 1427 ACCGGT EaeI 78 CGGCCG [CT]^GGCC[GA] 2600 TGGCCA EcoRI 527 GAATTC G^AATTC 2125 GAATTC EcoT22I 2725 ATGCAT ATGCA^T 2843 ATGCAT FbaI 1068 TGATCA T^GATCA 3411 TGATCA MaeII 1288 ACGT A^CGT 1914 ACGT NsiI 2725 ATGCAT ATGCA^T 2843 ATGCAT PflMI 239 CCAATATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 265 CCATCTGGTGG PstI 1307 CTGCAG CTGCA^G 3430 CTGCAG SapI 1346 GAAGAGC GCTCTTC(1/4) 629 GCTCTTC Van91I 239 CCAATATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 265 CCATCTGGTGG XmnI 753 GAATTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1572 GAAATTATTC ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AseI AsnI AvaII BbsI BbvI BfaI BmyI BpuAI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NdeI NdeII NlaIII NlaIV NspI PleI PshBI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI