##################################
# NO CUT ENZYMES (   78 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AocI        Aor51HI     ApaI        AscI        Asp718I     
AspI        AviII       AvrII       BamHI       BbeI        
BbvCI       BcgI        BglII       BlnI        BsaHI       
BseAI       BsiWI       BsmBI       BsmI        BspEI       
BssHII      Bst1107I    BstBI       BstZ17I     Bsu36I      
CpoI        DraII       DraIII      DrdI        Eco47III    
Eco81I      EcoNI       EcoO109I    EcoRV       FseI        
FspI        HgaI        Hin1I       HpaI        KasI        
KpnI        MluI        MroI        NarI        NcoI        
NheI        NruI        NspV        PacI        PflFI       
PmeI        PpuMI       Psp1406I    PvuI        RsrII       
SacI        SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SphI        Sse8387I    SwaI        Tth111I     
XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   75 enzymes) #
###################################

AccII          13  CGCG                 CG^CG
AflII        2271  CTTAAG               C^TTAAG
AflIII       3219  ACATGT               A^C[GA][CT]GT
AgeI         1427  ACCGGT               A^CCGGT
AhdI          173  GACCGAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        803  GTGCAC               G^TGCAC
ApaLI         803  GTGCAC               G^TGCAC
AspEI         173  GACCGAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI         803  GTGCAC               G[AT]GC[AT]^C
BalI         2600  TGGCCA               TGG^CCA
BanI         1430  GGTGCC               G^G[CT][GA]CC
BanII        1918  GAGCCC               G[GA]GC[CT]^C
BbrPI        1913  CACGTG               CAC^GTG
BciVI         249  GGATAC               GTATCC(6/5)
BcnI           20  CCGGG                CC^[GC]GG
BfrI         2271  CTTAAG               C^TTAAG
BglI           19  GCCGGGGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI          605  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      605  GCTCAGC              GC^T[ACGT]AGC
BsaAI        1913  CACGTG               [CT]AC^GT[GA]
BsaBI         697  GATTCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI          871  GAGACC               GGTCTC(1/5)
BsaWI        1427  ACCGGT               [AT]^CCGG[AT]
BsgI           67  CTGCAC               GTGCAG(16/14)
BsiHKAI       803  GTGCAC               G[AT]GC[AT]^C
Bsp1407I     3458  TGTACA               T^GTACA
BspDI        1122  ATCGAT               AT^CGAT
BspHI        2463  TCATGA               T^CATGA
BspLU11I     3219  ACATGT               A^CATGT
BsrBI           3  GAGCGG               CCGCTC(-3/-3)
BsrGI        3458  TGTACA               T^GTACA
BssSI         517  CACGAG               CACGAG(-5/-1)
BstEII        190  GGTCACC              G^GT[ACGT]ACC
BstPI         190  GGTCACC              G^GT[ACGT]ACC
BstUI          13  CGCG                 CG^CG
BstYI        2378  GGATCT               [GA]^GATC[CT]
CelII         605  GCTCAGC              GC^T[ACGT]AGC
CfoI         3074  GCGC                 GCG^C
ClaI         1122  ATCGAT               AT^CGAT
DsaI           12  CCGCGG               C^C[GA][CT]GG
EagI           78  CGGCCG               C^GGCCG
Eam1105I      173  GACCGAGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI          78  CGGCCG               C^GGCCG
Eco52I         78  CGGCCG               C^GGCCG
EcoO65I       190  GGTCACC              G^GT[ACGT]ACC
HaeII        3073  AGCGCC               [GA]GCGC^[CT]
HhaI         3074  GCGC                 GCG^C
HinP1I       3074  GCGC                 G^CGC
HincII       3328  GTCAAC               GT[CT]^[GA]AC
HindII       3328  GTCAAC               GT[CT]^[GA]AC
HindIII      1340  AAGCTT               A^AGCTT
KspI           12  CCGCGG               CCGC^GG
MamI          697  GATTCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2355  CAATTG               C^AATTG
MflI         2378  GGATCT               [GA]^GATC[CT]
MluNI        2600  TGGCCA               TGG^CCA
MscI         2600  TGGCCA               TGG^CCA
MunI         2355  CAATTG               C^AATTG
MvnI           13  CGCG                 CG^CG
NaeI         1193  GCCGGC               GCC^GGC
NciI           20  CCGGG                CC^[GC]GG
NgoMIV       1193  GCCGGC               G^CCGGC
NotI           77  GCGGCCGC             GC^GGCCGC
PaeR7I        840  CTCGAG               C^TCGAG
PinAI        1427  ACCGGT               A^CCGGT
PmaCI        1913  CACGTG               CAC^GTG
PmlI         1913  CACGTG               CAC^GTG
PshAI        3383  GACTAATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         2463  TCATGA               T^CATGA
SacII          12  CCGCGG               CCGC^GG
SpeI         3361  ACTAGT               A^CTAGT
SspBI        3458  TGTACA               T^GTACA
StuI         2664  AGGCCT               AGG^CCT
XhoI          840  CTCGAG               C^TCGAG
XhoII        2378  GGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI         1184  GTAGAC               GT^[AC][GT]AC
             3272  GTCTAC              
Asp700I       753  GAATTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1572  GAAATTATTC          
AvaI          840  CTCGAG               C^[CT]CG[GA]G
             2446  CCCGAG              
BclI         1068  TGATCA               T^GATCA
             3411  TGATCA              
BpmI          340  CTGGAG               CTGGAG(16/14)
             1888  CTGGAG              
BseRI          57  GAGGAG               GAGGAG(10/8)
             2368  GAGGAG              
BsiEI           6  CGGTCG               CG[GA][CT]^CG
               78  CGGCCG              
BsoBI         840  CTCGAG               C^[CT]CG[GA]G
             2446  CCCGAG              
BspMI        1903  ACCTGC               ACCTGC(4/8)
              275  GCAGGT              
BsrDI        2959  CATTGC               GCAATG(2/0)
              431  GCAATG              
BsrFI        1193  GCCGGC               [GA]^CCGG[CT]
             1427  ACCGGT              
BstAPI        796  GCAGATTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1230  GCAGCAACTGC         
BstXI        1619  CCAAGGGACTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3359  CCACTAGTGTGG        
Cfr10I       1193  GCCGGC               [GA]^CCGG[CT]
             1427  ACCGGT              
EaeI           78  CGGCCG               [CT]^GGCC[GA]
             2600  TGGCCA              
EcoRI         527  GAATTC               G^AATTC
             2125  GAATTC              
EcoT22I      2725  ATGCAT               ATGCA^T
             2843  ATGCAT              
FbaI         1068  TGATCA               T^GATCA
             3411  TGATCA              
MaeII        1288  ACGT                 A^CGT
             1914  ACGT                
NsiI         2725  ATGCAT               ATGCA^T
             2843  ATGCAT              
PflMI         239  CCAATATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              265  CCATCTGGTGG         
PstI         1307  CTGCAG               CTGCA^G
             3430  CTGCAG              
SapI         1346  GAAGAGC              GCTCTTC(1/4)
              629  GCTCTTC             
Van91I        239  CCAATATTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              265  CCATCTGGTGG         
XmnI          753  GAATTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1572  GAAATTATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AseI        AsnI        AvaII       
BbsI        BbvI        BfaI        BmyI        BpuAI       
BsaJI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       DraI        
EarI        EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NdeI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PshBI       PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SspI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XbaI