################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AatII AccII AclI AflII AflIII AgeI Aor51HI AscI AseI AsnI AspI BbeI BbrPI BciVI BclI BfrI BglII BsaBI BsaI BsgI BsiEI BsiWI BspDI BspLU11I BssHII BstBI BstUI ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI HaeII HincII HindII HpaI KasI KspI MamI MfeI MluI MseI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspI SwaI Tru9I Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### Acc65I 3210 GGTACC G^GTACC AccI 917 GTATAC GT^[AC][GT]AC AccIII 1301 TCCGGA T^CCGGA AcsI 3427 AAATTC [GA]^AATT[CT] AcyI 74 GGCGTC G[GA]^CG[CT]C Alw44I 1957 GTGCAC G^TGCAC ApaLI 1957 GTGCAC G^TGCAC ApoI 3427 AAATTC [GA]^AATT[CT] Asp718I 3210 GGTACC G^GTACC AviII 1086 TGCGCA TGC^GCA BcgI 1162 GCATGAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaHI 74 GGCGTC G[GA]^CG[CT]C BseAI 1301 TCCGGA T^CCGGA Bsp1407I 1832 TGTACA T^GTACA BspEI 1301 TCCGGA T^CCGGA BspHI 2799 TCATGA T^CATGA BsrGI 1832 TGTACA T^GTACA BssSI 965 CACGAG CACGAG(-5/-1) Bst1107I 917 GTATAC GTA^TAC BstEII 2448 GGTCACC G^GT[ACGT]ACC BstPI 2448 GGTCACC G^GT[ACGT]ACC BstZ17I 917 GTATAC GTA^TAC EcoO65I 2448 GGTCACC G^GT[ACGT]ACC FspI 1086 TGCGCA TGC^GCA Hin1I 74 GGCGTC G[GA]^CG[CT]C HindIII 560 AAGCTT A^AGCTT KpnI 3210 GGTACC GGTAC^C MroI 1301 TCCGGA T^CCGGA RcaI 2799 TCATGA T^CATGA SexAI 819 ACCTGGT A^CC[AT]GGT SspBI 1832 TGTACA T^GTACA StuI 1575 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AhdI 62 GACTACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 722 GACATCGTGTC AocI 42 CCTCAGG CC^T[ACGT]AGG 1196 CCTAAGG Asp700I 8 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 779 GAACCCGTTC AspEI 62 GACTACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 722 GACATCGTGTC AvrII 715 CCTAGG C^CTAGG 3271 CCTAGG BamHI 1192 GGATCC G^GATCC 2001 GGATCC BglI 2186 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3241 GCCGGGGAGGC BlnI 715 CCTAGG C^CTAGG 3271 CCTAGG BsaAI 134 TACGTG [CT]AC^GT[GA] 1906 TACGTG BsaWI 1301 TCCGGA [AT]^CCGG[AT] 1808 ACCGGA BsmBI 428 GAGACG CGTCTC(1/5) 1858 GAGACG BsmI 682 GAATGC GAATGC(1/-1) 3094 GCATTC BsrBI 2679 CCGCTC CCGCTC(-3/-3) 1641 GAGCGG BsrDI 760 CATTGC GCAATG(2/0) 2865 CATTGC BstAPI 1177 GCAGGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3330 GCAGACTCTGC Bsu36I 42 CCTCAGG CC^T[ACGT]AGG 1196 CCTAAGG Eam1105I 62 GACTACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 722 GACATCGTGTC Eco81I 42 CCTCAGG CC^T[ACGT]AGG 1196 CCTAAGG EcoNI 2619 CCTGGCATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2987 CCTTGGGTAGG SacI 395 GAGCTC GAGCT^C 1371 GAGCTC SapI 1277 GAAGAGC GCTCTTC(1/4) 3437 GAAGAGC SpeI 2551 ACTAGT A^CTAGT 3396 ACTAGT Tsp509I 2836 AATT ^AATT 3428 AATT XmnI 8 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 779 GAACCCGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII