##################################
# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII AccII AclI AflII AflIII
AgeI Aor51HI AscI AseI AsnI
AspI BbeI BbrPI BciVI BclI
BfrI BglII BsaBI BsaI BsgI
BsiEI BsiWI BspDI BspLU11I BssHII
BstBI BstUI ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FseI HaeII HincII HindII HpaI
KasI KspI MamI MfeI MluI
MseI MunI MvnI NarI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspI SwaI Tru9I Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 3210 GGTACC G^GTACC
AccI 917 GTATAC GT^[AC][GT]AC
AccIII 1301 TCCGGA T^CCGGA
AcsI 3427 AAATTC [GA]^AATT[CT]
AcyI 74 GGCGTC G[GA]^CG[CT]C
Alw44I 1957 GTGCAC G^TGCAC
ApaLI 1957 GTGCAC G^TGCAC
ApoI 3427 AAATTC [GA]^AATT[CT]
Asp718I 3210 GGTACC G^GTACC
AviII 1086 TGCGCA TGC^GCA
BcgI 1162 GCATGAGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 74 GGCGTC G[GA]^CG[CT]C
BseAI 1301 TCCGGA T^CCGGA
Bsp1407I 1832 TGTACA T^GTACA
BspEI 1301 TCCGGA T^CCGGA
BspHI 2799 TCATGA T^CATGA
BsrGI 1832 TGTACA T^GTACA
BssSI 965 CACGAG CACGAG(-5/-1)
Bst1107I 917 GTATAC GTA^TAC
BstEII 2448 GGTCACC G^GT[ACGT]ACC
BstPI 2448 GGTCACC G^GT[ACGT]ACC
BstZ17I 917 GTATAC GTA^TAC
EcoO65I 2448 GGTCACC G^GT[ACGT]ACC
FspI 1086 TGCGCA TGC^GCA
Hin1I 74 GGCGTC G[GA]^CG[CT]C
HindIII 560 AAGCTT A^AGCTT
KpnI 3210 GGTACC GGTAC^C
MroI 1301 TCCGGA T^CCGGA
RcaI 2799 TCATGA T^CATGA
SexAI 819 ACCTGGT A^CC[AT]GGT
SspBI 1832 TGTACA T^GTACA
StuI 1575 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AhdI 62 GACTACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
722 GACATCGTGTC
AocI 42 CCTCAGG CC^T[ACGT]AGG
1196 CCTAAGG
Asp700I 8 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
779 GAACCCGTTC
AspEI 62 GACTACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
722 GACATCGTGTC
AvrII 715 CCTAGG C^CTAGG
3271 CCTAGG
BamHI 1192 GGATCC G^GATCC
2001 GGATCC
BglI 2186 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3241 GCCGGGGAGGC
BlnI 715 CCTAGG C^CTAGG
3271 CCTAGG
BsaAI 134 TACGTG [CT]AC^GT[GA]
1906 TACGTG
BsaWI 1301 TCCGGA [AT]^CCGG[AT]
1808 ACCGGA
BsmBI 428 GAGACG CGTCTC(1/5)
1858 GAGACG
BsmI 682 GAATGC GAATGC(1/-1)
3094 GCATTC
BsrBI 2679 CCGCTC CCGCTC(-3/-3)
1641 GAGCGG
BsrDI 760 CATTGC GCAATG(2/0)
2865 CATTGC
BstAPI 1177 GCAGGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3330 GCAGACTCTGC
Bsu36I 42 CCTCAGG CC^T[ACGT]AGG
1196 CCTAAGG
Eam1105I 62 GACTACAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
722 GACATCGTGTC
Eco81I 42 CCTCAGG CC^T[ACGT]AGG
1196 CCTAAGG
EcoNI 2619 CCTGGCATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2987 CCTTGGGTAGG
SacI 395 GAGCTC GAGCT^C
1371 GAGCTC
SapI 1277 GAAGAGC GCTCTTC(1/4)
3437 GAAGAGC
SpeI 2551 ACTAGT A^CTAGT
3396 ACTAGT
Tsp509I 2836 AATT ^AATT
3428 AATT
XmnI 8 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
779 GAACCCGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI TseI Tsp45I TspRI
TthHB8I Van91I XcmI XhoII