##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AclI AflII AgeI AhdI AscI
AseI AsnI AspEI BamHI BfrI
BsaBI BsiWI BspDI BspLU11I BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco52I EcoO65I EcoRV
EcoT22I FseI HpaI MamI MfeI
MunI NdeI NotI NruI NsiI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I RsrII SalI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 2202 GACGTC GACGT^C
Acc65I 3214 GGTACC G^GTACC
AcsI 543 GAATTC [GA]^AATT[CT]
AflIII 2256 ACGCGT A^C[GA][CT]GT
ApoI 543 GAATTC [GA]^AATT[CT]
Asp700I 965 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3214 GGTACC G^GTACC
AspI 2199 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2261 TGCGCA TGC^GCA
AvrII 3694 CCTAGG C^CTAGG
BbeI 1723 GGCGCC GGCGC^C
BbrPI 2193 CACGTG CAC^GTG
BcgI 3447 GCACAGGAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3976 GGATAC GTATCC(6/5)
BclI 295 TGATCA T^GATCA
BglII 2314 AGATCT A^GATCT
BlnI 3694 CCTAGG C^CTAGG
BsiEI 3026 CGATCG CG[GA][CT]^CG
BsmBI 2204 CGTCTC CGTCTC(1/5)
BsmI 1625 GAATGC GAATGC(1/-1)
BspHI 1640 TCATGA T^CATGA
EcoRI 543 GAATTC G^AATTC
FbaI 295 TGATCA T^GATCA
FspI 2261 TGCGCA TGC^GCA
KasI 1723 GGCGCC G^GCGCC
KpnI 3214 GGTACC GGTAC^C
KspI 857 CCGCGG CCGC^GG
MluI 2256 ACGCGT A^CGCGT
MseI 2698 TTAA T^TAA
NarI 1723 GGCGCC GG^CGCC
NheI 1697 GCTAGC G^CTAGC
PaeR7I 3310 CTCGAG C^TCGAG
PflFI 2199 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2193 CACGTG CAC^GTG
PmlI 2193 CACGTG CAC^GTG
PvuI 3026 CGATCG CGAT^CG
RcaI 1640 TCATGA T^CATGA
SacII 857 CCGCGG CCGC^GG
SbfI 3657 CCTGCAGG CCTGCA^GG
SfoI 1723 GGCGCC GGC^GCC
SmlI 3310 CTCGAG C^T[CT][GA]AG
SphI 2633 GCATGC GCATG^C
Sse8387I 3657 CCTGCAGG CCTGCA^GG
StuI 2284 AGGCCT AGG^CCT
Tru9I 2698 TTAA T^TAA
Tth111I 2199 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 3310 CTCGAG C^TCGAG
XmnI 965 GAACCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 858 CGCG CG^CG
2257 CGCG
Alw44I 2169 GTGCAC G^TGCAC
3180 GTGCAC
AocI 2228 CCTCAGG CC^T[ACGT]AGG
3330 CCTGAGG
Aor51HI 232 AGCGCT AGC^GCT
838 AGCGCT
ApaI 1818 GGGCCC GGGCC^C
3770 GGGCCC
ApaLI 2169 GTGCAC G^TGCAC
3180 GTGCAC
BalI 6 TGGCCA TGG^CCA
2932 TGGCCA
BbvCI 1686 GCTGAGG CCTCAGC(-5/-2)
2561 GCTGAGG
BlpI 738 GCTGAGC GC^T[ACGT]AGC
1236 GCTGAGC
Bpu1102I 738 GCTGAGC GC^T[ACGT]AGC
1236 GCTGAGC
BsaI 277 GAGACC GGTCTC(1/5)
1155 GAGACC
BstAPI 359 GCAGGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3664 GCAAGCAATGC
BstUI 858 CGCG CG^CG
2257 CGCG
BstYI 2314 AGATCT [GA]^GATC[CT]
2701 AGATCC
Bsu36I 2228 CCTCAGG CC^T[ACGT]AGG
3330 CCTGAGG
CelII 738 GCTGAGC GC^T[ACGT]AGC
1236 GCTGAGC
DraIII 2069 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
2526 CACTACGTG
Eco47III 232 AGCGCT AGC^GCT
838 AGCGCT
Eco81I 2228 CCTCAGG CC^T[ACGT]AGG
3330 CCTGAGG
FauI 2825 CCCGC CCCGC(4/6)
3774 CCCGC
HincII 2355 GTCAAC GT[CT]^[GA]AC
2799 GTCAAC
HindII 2355 GTCAAC GT[CT]^[GA]AC
2799 GTCAAC
MflI 2314 AGATCT [GA]^GATC[CT]
2701 AGATCC
MluNI 6 TGGCCA TGG^CCA
2932 TGGCCA
MscI 6 TGGCCA TGG^CCA
2932 TGGCCA
MvnI 858 CGCG CG^CG
2257 CGCG
NspI 2633 GCATGC [GA]CATG^[CT]
2693 GCATGT
PvuII 1190 CAGCTG CAG^CTG
3050 CAGCTG
Tsp509I 544 AATT ^AATT
3722 AATT
XhoII 2314 AGATCT [GA]^GATC[CT]
2701 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccIII AciI AcyI AfaI
AluI AlwI AlwNI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaJI BsaWI
BseAI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssKI BssSI
BstF5I BstNI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MroI MslI
MspA1I MspI MvaI MwoI NaeI
NciI NcoI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PpuMI PstI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SspBI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I Van91I XcmI XmaCI
XmaI