################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII AccIII AclI AgeI ApaI AscI AseI AsnI AviII BamHI BcgI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BsrDI BsrGI BssHII Bst1107I BstZ17I ClaI CpoI EcoRI EcoRV EcoT22I FspI HindIII KspI MfeI MluI MroI MunI NdeI NotI NruI NsiI PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### Acc65I 156 GGTACC G^GTACC AccII 1661 CGCG CG^CG AocI 244 CCTGAGG CC^T[ACGT]AGG Aor51HI 3114 AGCGCT AGC^GCT Asp700I 337 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 156 GGTACC G^GTACC AspI 780 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 934 TGGCCA TGG^CCA BbeI 1058 GGCGCC GGCGC^C BbrPI 2042 CACGTG CAC^GTG BbsI 3477 GAAGAC GAAGAC(2/6) BciVI 2568 GTATCC GTATCC(6/5) BclI 307 TGATCA T^GATCA BglII 1135 AGATCT A^GATCT BpuAI 3477 GAAGAC GAAGAC(2/6) BsaAI 2042 CACGTG [CT]AC^GT[GA] BsaI 240 GAGACC GGTCTC(1/5) BsiEI 1531 CGGCCG CG[GA][CT]^CG BsmBI 3622 CGTCTC CGTCTC(1/5) BsmI 4097 GCATTC GAATGC(1/-1) BssSI 2829 CACGAG CACGAG(-5/-1) BstAPI 2791 GCAGTCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 2938 TTCGAA TT^CGAA BstEII 1274 GGTCACC G^GT[ACGT]ACC BstPI 1274 GGTCACC G^GT[ACGT]ACC BstUI 1661 CGCG CG^CG BstXI 1970 CCAGAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 244 CCTGAGG CC^T[ACGT]AGG DraI 2082 TTTAAA TTT^AAA EagI 1531 CGGCCG C^GGCCG EclXI 1531 CGGCCG C^GGCCG Eco47III 3114 AGCGCT AGC^GCT Eco52I 1531 CGGCCG C^GGCCG Eco81I 244 CCTGAGG CC^T[ACGT]AGG EcoO65I 1274 GGTCACC G^GT[ACGT]ACC FbaI 307 TGATCA T^GATCA FseI 1532 GGCCGGCC GGCCGG^CC HpaI 2401 GTTAAC GTT^AAC KasI 1058 GGCGCC G^GCGCC KpnI 156 GGTACC GGTAC^C MluNI 934 TGGCCA TGG^CCA MscI 934 TGGCCA TGG^CCA MvnI 1661 CGCG CG^CG NarI 1058 GGCGCC GG^CGCC NheI 3525 GCTAGC G^CTAGC NspV 2938 TTCGAA TT^CGAA PflFI 780 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2042 CACGTG CAC^GTG PmlI 2042 CACGTG CAC^GTG SacI 2361 GAGCTC GAGCT^C SfiI 887 GGCCCATGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1058 GGCGCC GGC^GCC SfuI 2938 TTCGAA TT^CGAA SspI 2002 AATATT AAT^ATT Tth111I 780 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 2395 TCTAGA T^CTAGA XcmI 2770 CCATGGACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 337 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 3447 GTAGAC GT^[AC][GT]AC 4057 GTCTAC AcyI 651 GGCGTC G[GA]^CG[CT]C 1058 GGCGCC Alw44I 393 GTGCAC G^TGCAC 2045 GTGCAC ApaLI 393 GTGCAC G^TGCAC 2045 GTGCAC AvrII 1116 CCTAGG C^CTAGG 2464 CCTAGG BlnI 1116 CCTAGG C^CTAGG 2464 CCTAGG BsaBI 2249 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2563 GATTTGTATC BsaHI 651 GGCGTC G[GA]^CG[CT]C 1058 GGCGCC BsgI 643 GTGCAG GTGCAG(16/14) 3267 GTGCAG BspHI 449 TCATGA T^CATGA 1835 TCATGA BspLU11I 409 ACATGT A^CATGT 518 ACATGT BsrFI 1533 GCCGGC [GA]^CCGG[CT] 3220 GCCGGC Cfr10I 1533 GCCGGC [GA]^CCGG[CT] 3220 GCCGGC DrdI 70 GACCCTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 780 GACAACGTCGTC HaeII 1058 GGCGCC [GA]GCGC^[CT] 3114 AGCGCT Hin1I 651 GGCGTC G[GA]^CG[CT]C 1058 GGCGCC HincII 983 GTTGAC GT[CT]^[GA]AC 2401 GTTAAC HindII 983 GTTGAC GT[CT]^[GA]AC 2401 GTTAAC MamI 2249 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2563 GATTTGTATC NaeI 1533 GCCGGC GCC^GGC 3220 GCCGGC NgoMIV 1533 GCCGGC G^CCGGC 3220 GCCGGC RcaI 449 TCATGA T^CATGA 1835 TCATGA SapI 3581 GAAGAGC GCTCTTC(1/4) 3242 GCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AciI AcsI AfaI AflII AflIII AhdI AluI AlwI AlwNI ApoI AspEI AspHI AvaI AvaII BanI BanII BbvCI BbvI BcnI BfaI BfrI BglI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI Eam1105I EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII