##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccIII AclI AgeI ApaI
AscI AseI AsnI AviII BamHI
BcgI BsaWI BseAI BsiWI Bsp1407I
BspDI BspEI BsrDI BsrGI BssHII
Bst1107I BstZ17I ClaI CpoI EcoRI
EcoRV EcoT22I FspI HindIII KspI
MfeI MluI MroI MunI NdeI
NotI NruI NsiI PacI PaeR7I
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 156 GGTACC G^GTACC
AccII 1661 CGCG CG^CG
AocI 244 CCTGAGG CC^T[ACGT]AGG
Aor51HI 3114 AGCGCT AGC^GCT
Asp700I 337 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 156 GGTACC G^GTACC
AspI 780 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 934 TGGCCA TGG^CCA
BbeI 1058 GGCGCC GGCGC^C
BbrPI 2042 CACGTG CAC^GTG
BbsI 3477 GAAGAC GAAGAC(2/6)
BciVI 2568 GTATCC GTATCC(6/5)
BclI 307 TGATCA T^GATCA
BglII 1135 AGATCT A^GATCT
BpuAI 3477 GAAGAC GAAGAC(2/6)
BsaAI 2042 CACGTG [CT]AC^GT[GA]
BsaI 240 GAGACC GGTCTC(1/5)
BsiEI 1531 CGGCCG CG[GA][CT]^CG
BsmBI 3622 CGTCTC CGTCTC(1/5)
BsmI 4097 GCATTC GAATGC(1/-1)
BssSI 2829 CACGAG CACGAG(-5/-1)
BstAPI 2791 GCAGTCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2938 TTCGAA TT^CGAA
BstEII 1274 GGTCACC G^GT[ACGT]ACC
BstPI 1274 GGTCACC G^GT[ACGT]ACC
BstUI 1661 CGCG CG^CG
BstXI 1970 CCAGAGTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 244 CCTGAGG CC^T[ACGT]AGG
DraI 2082 TTTAAA TTT^AAA
EagI 1531 CGGCCG C^GGCCG
EclXI 1531 CGGCCG C^GGCCG
Eco47III 3114 AGCGCT AGC^GCT
Eco52I 1531 CGGCCG C^GGCCG
Eco81I 244 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1274 GGTCACC G^GT[ACGT]ACC
FbaI 307 TGATCA T^GATCA
FseI 1532 GGCCGGCC GGCCGG^CC
HpaI 2401 GTTAAC GTT^AAC
KasI 1058 GGCGCC G^GCGCC
KpnI 156 GGTACC GGTAC^C
MluNI 934 TGGCCA TGG^CCA
MscI 934 TGGCCA TGG^CCA
MvnI 1661 CGCG CG^CG
NarI 1058 GGCGCC GG^CGCC
NheI 3525 GCTAGC G^CTAGC
NspV 2938 TTCGAA TT^CGAA
PflFI 780 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2042 CACGTG CAC^GTG
PmlI 2042 CACGTG CAC^GTG
SacI 2361 GAGCTC GAGCT^C
SfiI 887 GGCCCATGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1058 GGCGCC GGC^GCC
SfuI 2938 TTCGAA TT^CGAA
SspI 2002 AATATT AAT^ATT
Tth111I 780 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2395 TCTAGA T^CTAGA
XcmI 2770 CCATGGACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 337 GAAAATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 3447 GTAGAC GT^[AC][GT]AC
4057 GTCTAC
AcyI 651 GGCGTC G[GA]^CG[CT]C
1058 GGCGCC
Alw44I 393 GTGCAC G^TGCAC
2045 GTGCAC
ApaLI 393 GTGCAC G^TGCAC
2045 GTGCAC
AvrII 1116 CCTAGG C^CTAGG
2464 CCTAGG
BlnI 1116 CCTAGG C^CTAGG
2464 CCTAGG
BsaBI 2249 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2563 GATTTGTATC
BsaHI 651 GGCGTC G[GA]^CG[CT]C
1058 GGCGCC
BsgI 643 GTGCAG GTGCAG(16/14)
3267 GTGCAG
BspHI 449 TCATGA T^CATGA
1835 TCATGA
BspLU11I 409 ACATGT A^CATGT
518 ACATGT
BsrFI 1533 GCCGGC [GA]^CCGG[CT]
3220 GCCGGC
Cfr10I 1533 GCCGGC [GA]^CCGG[CT]
3220 GCCGGC
DrdI 70 GACCCTGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
780 GACAACGTCGTC
HaeII 1058 GGCGCC [GA]GCGC^[CT]
3114 AGCGCT
Hin1I 651 GGCGTC G[GA]^CG[CT]C
1058 GGCGCC
HincII 983 GTTGAC GT[CT]^[GA]AC
2401 GTTAAC
HindII 983 GTTGAC GT[CT]^[GA]AC
2401 GTTAAC
MamI 2249 GATAGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2563 GATTTGTATC
NaeI 1533 GCCGGC GCC^GGC
3220 GCCGGC
NgoMIV 1533 GCCGGC G^CCGGC
3220 GCCGGC
RcaI 449 TCATGA T^CATGA
1835 TCATGA
SapI 3581 GAAGAGC GCTCTTC(1/4)
3242 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AhdI AluI AlwI AlwNI ApoI
AspEI AspHI AvaI AvaII BanI
BanII BbvCI BbvI BcnI BfaI
BfrI BglI BlpI BmyI BpmI
Bpu1102I BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI Eam1105I EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII