##################################
# NO CUT ENZYMES ( 52 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
BcgI BfrI BglII BsiWI BsmI
BspDI BsrBI BstBI ClaI CpoI
DraI DrdI Eam1105I Eco47III EcoRI
EcoRV HindIII HpaI MfeI MluI
MunI NheI NotI NruI NspV
PacI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI SapI
SfiI SfuI SgrAI SnaBI SpeI
SphI SwaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 859 GGTACC G^GTACC
AccIII 3714 TCCGGA T^CCGGA
AcsI 258 AAATTT [GA]^AATT[CT]
ApaI 228 GGGCCC GGGCC^C
ApoI 258 AAATTT [GA]^AATT[CT]
Asp718I 859 GGTACC G^GTACC
AviII 4252 TGCGCA TGC^GCA
BbeI 136 GGCGCC GGCGC^C
BbrPI 1403 CACGTG CAC^GTG
BlpI 5144 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 5144 GCTGAGC GC^T[ACGT]AGC
BsaBI 3795 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 2718 GGTCTC GGTCTC(1/5)
BsaWI 3714 TCCGGA [AT]^CCGG[AT]
BseAI 3714 TCCGGA T^CCGGA
BsmBI 568 GAGACG CGTCTC(1/5)
Bsp1407I 4343 TGTACA T^GTACA
BspEI 3714 TCCGGA T^CCGGA
BspHI 2509 TCATGA T^CATGA
BsrGI 4343 TGTACA T^GTACA
CelII 5144 GCTGAGC GC^T[ACGT]AGC
EcoNI 817 CCTCGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 606 GGCCGGCC GGCCGG^CC
FspI 4252 TGCGCA TGC^GCA
HincII 3619 GTTGAC GT[CT]^[GA]AC
HindII 3619 GTTGAC GT[CT]^[GA]AC
KasI 136 GGCGCC G^GCGCC
KpnI 859 GGTACC GGTAC^C
KspI 131 CCGCGG CCGC^GG
MamI 3795 GATGAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3714 TCCGGA T^CCGGA
NarI 136 GGCGCC GG^CGCC
NdeI 4492 CATATG CA^TATG
PaeR7I 809 CTCGAG C^TCGAG
PmaCI 1403 CACGTG CAC^GTG
PmlI 1403 CACGTG CAC^GTG
RcaI 2509 TCATGA T^CATGA
SacII 131 CCGCGG CCGC^GG
SbfI 117 CCTGCAGG CCTGCA^GG
ScaI 3224 AGTACT AGT^ACT
SexAI 998 ACCTGGT A^CC[AT]GGT
SfoI 136 GGCGCC GGC^GCC
SmaI 3763 CCCGGG CCC^GGG
Sse8387I 117 CCTGCAGG CCTGCA^GG
SspBI 4343 TGTACA T^GTACA
SspI 1378 AATATT AAT^ATT
XhoI 809 CTCGAG C^TCGAG
XmaCI 3763 CCCGGG C^CCGGG
XmaI 3763 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 136 GGCGCC G[GA]^CG[CT]C
2761 GGCGTC
AlwNI 3586 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
4906 CAGTGTCTG
Asp700I 1665 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4061 GAATGTCTTC
BbvCI 2367 CCTCAGC CCTCAGC(-5/-2)
1507 GCTGAGG
BciVI 3384 GGATAC GTATCC(6/5)
3865 GGATAC
BglI 611 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
874 GCCCCCCAGGC
BsaHI 136 GGCGCC G[GA]^CG[CT]C
2761 GGCGTC
BssHII 94 GCGCGC G^CGCGC
1398 GCGCGC
BssSI 179 CACGAG CACGAG(-5/-1)
4110 CTCGTG
BstEII 1239 GGTGACC G^GT[ACGT]ACC
2727 GGTTACC
BstPI 1239 GGTGACC G^GT[ACGT]ACC
2727 GGTTACC
EagI 38 CGGCCG C^GGCCG
605 CGGCCG
EclXI 38 CGGCCG C^GGCCG
605 CGGCCG
Eco52I 38 CGGCCG C^GGCCG
605 CGGCCG
EcoO65I 1239 GGTGACC G^GT[ACGT]ACC
2727 GGTTACC
EcoT22I 3487 ATGCAT ATGCA^T
4532 ATGCAT
Hin1I 136 GGCGCC G[GA]^CG[CT]C
2761 GGCGTC
NsiI 3487 ATGCAT ATGCA^T
4532 ATGCAT
PflMI 1843 CCATAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2046 CCAGGCAGTGG
Van91I 1843 CCATAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2046 CCAGGCAGTGG
XmnI 1665 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4061 GAATGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 136 enzymes) #
#########################################
AccI AccII AciI AfaI AflIII
AluI Alw44I AlwI AocI ApaLI
AspHI AspI AvaI AvaII AvrII
BalI BamHI BanI BanII BbsI
BbvI BclI BcnI BfaI BlnI
BmyI BpmI BpuAI BsaAI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspLU11I BspMI BsrDI BsrFI BsrI
BssKI Bst1107I BstAPI BstF5I BstNI
BstUI BstXI BstYI BstZ17I Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflFI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I XbaI XcmI
XhoII