##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AgeI ApaI AscI AseI AsnI
Asp718I AviII AvrII BbeI BclI
BfrI BlnI BsaHI BseAI BsiEI
BsiWI BspDI BspEI BssHII BstBI
BstEII BstPI ClaI CpoI DrdI
EagI EclXI Eco52I EcoO65I FbaI
FseI FspI Hin1I HpaI KasI
KpnI KspI MfeI MluI MroI
MunI NarI NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI PvuI RsrII SacII SalI
SexAI SfoI SfuI SgrAI SspI
StuI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccII 3649 CGCG CG^CG
AclI 160 AACGTT AA^CGTT
AhdI 2113 GACTTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 2113 GACTTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2752 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 3061 GGATCC G^GATCC
BbrPI 2244 CACGTG CAC^GTG
BcgI 4994 CGAGTTGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 659 GCCAATGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 3789 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3055 ACCGGA [AT]^CCGG[AT]
BsmBI 212 GAGACG CGTCTC(1/5)
BspHI 594 TCATGA T^CATGA
BsrFI 2257 GCCGGC [GA]^CCGG[CT]
BssSI 4992 CACGAG CACGAG(-5/-1)
BstUI 3649 CGCG CG^CG
Cfr10I 2257 GCCGGC [GA]^CCGG[CT]
Eam1105I 2113 GACTTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2865 CCTATTCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HindIII 33 AAGCTT A^AGCTT
MamI 3789 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 3649 CGCG CG^CG
NaeI 2257 GCCGGC GCC^GGC
NgoMIV 2257 GCCGGC G^CCGGC
NheI 965 GCTAGC G^CTAGC
PflFI 2752 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2244 CACGTG CAC^GTG
PmlI 2244 CACGTG CAC^GTG
Psp1406I 160 AACGTT AA^CGTT
RcaI 594 TCATGA T^CATGA
SbfI 694 CCTGCAGG CCTGCA^GG
SfiI 658 GGCCAATGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 151 CCCGGG CCC^GGG
SpeI 1986 ACTAGT A^CTAGT
Sse8387I 694 CCTGCAGG CCTGCA^GG
Tth111I 2752 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 151 CCCGGG C^CCGGG
XmaI 151 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AflIII 997 ACATGT A^C[GA][CT]GT
4417 ACATGT
AocI 3106 CCTCAGG CC^T[ACGT]AGG
4855 CCTCAGG
Aor51HI 3933 AGCGCT AGC^GCT
5391 AGCGCT
Asp700I 2690 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3596 GAATGTTTTC
AvaI 151 CCCGGG C^[CT]CG[GA]G
1166 CCCGAG
BbvCI 3478 CCTCAGC CCTCAGC(-5/-2)
5576 CCTCAGC
BglII 1844 AGATCT A^GATCT
2007 AGATCT
BlpI 1390 GCTGAGC GC^T[ACGT]AGC
5845 GCTCAGC
Bpu1102I 1390 GCTGAGC GC^T[ACGT]AGC
5845 GCTCAGC
BseRI 3266 CTCCTC GAGGAG(10/8)
605 GAGGAG
BsoBI 151 CCCGGG C^[CT]CG[GA]G
1166 CCCGAG
BspLU11I 997 ACATGT A^CATGT
4417 ACATGT
BsrBI 2339 CCGCTC CCGCTC(-3/-3)
2089 GAGCGG
Bst1107I 1795 GTATAC GTA^TAC
5252 GTATAC
BstZ17I 1795 GTATAC GTA^TAC
5252 GTATAC
Bsu36I 3106 CCTCAGG CC^T[ACGT]AGG
4855 CCTCAGG
CelII 1390 GCTGAGC GC^T[ACGT]AGC
5845 GCTCAGC
Eco47III 3933 AGCGCT AGC^GCT
5391 AGCGCT
Eco81I 3106 CCTCAGG CC^T[ACGT]AGG
4855 CCTCAGG
EcoRV 335 GATATC GAT^ATC
2734 GATATC
EcoT22I 4401 ATGCAT ATGCA^T
4437 ATGCAT
HgaI 179 GACGC GACGC(5/10)
735 GCGTC
HincII 2482 GTCAAC GT[CT]^[GA]AC
2588 GTCAAC
HindII 2482 GTCAAC GT[CT]^[GA]AC
2588 GTCAAC
NcoI 2133 CCATGG C^CATGG
2470 CCATGG
NsiI 4401 ATGCAT ATGCA^T
4437 ATGCAT
SacI 983 GAGCTC GAGCT^C
4642 GAGCTC
ScaI 2433 AGTACT AGT^ACT
4383 AGTACT
SnaBI 2236 TACGTA TAC^GTA
2266 TACGTA
XbaI 763 TCTAGA T^CTAGA
886 TCTAGA
XmnI 2690 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3596 GAATGTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
Alw44I AlwI AlwNI ApaLI ApoI
AspHI AvaII BalI BanI BanII
BbsI BbvI BciVI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI Bsp1286I Bsp1407I
BspMI BsrDI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI EcoO109I EcoRI EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII