##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AccII AccIII AclI AflII AgeI
Alw44I Aor51HI ApaLI AscI AviII
BbeI BcgI BfrI BsaBI BseAI
BsmI BspDI BspEI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoRI EcoRV FseI FspI
HindIII HpaI KasI KspI MamI
MluI MroI MvnI NaeI NarI
NgoMIV NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 995 GACGTC GACGT^C
AccI 3470 GTCTAC GT^[AC][GT]AC
AseI 3323 ATTAAT AT^TAAT
AsnI 3323 ATTAAT AT^TAAT
Asp700I 107 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1498 CCTAGG C^CTAGG
BalI 3568 TGGCCA TGG^CCA
BamHI 1558 GGATCC G^GATCC
BbrPI 2912 CACGTG CAC^GTG
BclI 2515 TGATCA T^GATCA
BglII 171 AGATCT A^GATCT
BlnI 1498 CCTAGG C^CTAGG
BsaI 1996 GGTCTC GGTCTC(1/5)
BsaWI 4750 TCCGGT [AT]^CCGG[AT]
BsgI 4865 GTGCAG GTGCAG(16/14)
BsiWI 3511 CGTACG C^GTACG
BspHI 4011 TCATGA T^CATGA
BsrFI 519 GCCGGT [GA]^CCGG[CT]
BstAPI 3618 GCATTATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3347 GGTAACC G^GT[ACGT]ACC
BstPI 3347 GGTAACC G^GT[ACGT]ACC
CfoI 2260 GCGC GCG^C
Cfr10I 519 GCCGGT [GA]^CCGG[CT]
DraI 4350 TTTAAA TTT^AAA
EcoO65I 3347 GGTAACC G^GT[ACGT]ACC
EcoT22I 1862 ATGCAT ATGCA^T
FbaI 2515 TGATCA T^GATCA
HaeII 2259 AGCGCC [GA]GCGC^[CT]
HhaI 2260 GCGC GCG^C
HinP1I 2260 GCGC G^CGC
HincII 320 GTCAAC GT[CT]^[GA]AC
HindII 320 GTCAAC GT[CT]^[GA]AC
MfeI 588 CAATTG C^AATTG
MluNI 3568 TGGCCA TGG^CCA
MscI 3568 TGGCCA TGG^CCA
MunI 588 CAATTG C^AATTG
NcoI 5384 CCATGG C^CATGG
NsiI 1862 ATGCAT ATGCA^T
PmaCI 2912 CACGTG CAC^GTG
PmlI 2912 CACGTG CAC^GTG
PshBI 3323 ATTAAT AT^TAAT
RcaI 4011 TCATGA T^CATGA
ScaI 614 AGTACT AGT^ACT
SexAI 1353 ACCTGGT A^CC[AT]GGT
XmnI 107 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 995 GACGTC G[GA]^CG[CT]C
1510 GGCGTC
AhdI 1631 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1800 GACCAAGTGTC
AspEI 1631 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1800 GACCAAGTGTC
AspI 1585 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2973 GACAAGGTC
BbvCI 815 CCTCAGC CCTCAGC(-5/-2)
539 GCTGAGG
BciVI 3426 GTATCC GTATCC(6/5)
3450 GTATCC
BlpI 1021 GCTGAGC GC^T[ACGT]AGC
5448 GCTCAGC
Bpu1102I 1021 GCTGAGC GC^T[ACGT]AGC
5448 GCTCAGC
BsaHI 995 GACGTC G[GA]^CG[CT]C
1510 GGCGTC
BsiEI 2233 CGACCG CG[GA][CT]^CG
2536 CGACCG
BsmBI 453 GAGACG CGTCTC(1/5)
1583 GAGACG
BspLU11I 1068 ACATGT A^CATGT
5056 ACATGT
BspMI 3179 ACCTGC ACCTGC(4/8)
5468 GCAGGT
CelII 1021 GCTGAGC GC^T[ACGT]AGC
5448 GCTCAGC
DsaI 240 CCGTGG C^C[GA][CT]GG
5384 CCATGG
EaeI 1540 TGGCCG [CT]^GGCC[GA]
3568 TGGCCA
Eam1105I 1631 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1800 GACCAAGTGTC
Hin1I 995 GACGTC G[GA]^CG[CT]C
1510 GGCGTC
NdeI 1605 CATATG CA^TATG
1887 CATATG
NheI 3995 GCTAGC G^CTAGC
5311 GCTAGC
PflFI 1585 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2973 GACAAGGTC
PflMI 2287 CCACCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3749 CCAGCATGTGG
PpuMI 765 AGGACCC [GA]G^G[AT]CC[CT]
2793 GGGACCC
PstI 4539 CTGCAG CTGCA^G
5210 CTGCAG
SspI 2640 AATATT AAT^ATT
4354 AATATT
Tth111I 1585 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2973 GACAAGGTC
Van91I 2287 CCACCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3749 CCAGCATGTGG
XcmI 4185 CCAGCAGGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5293 CCAAGATTCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
ApoI Asp718I AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BsrBI BsrDI BsrGI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraII DraIII EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SpeI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII