##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII AccIII AclI AcsI AflII
AgeI AhdI AocI ApoI AscI
AseI AsnI AspEI AspI AvrII
BamHI BbrPI BbvCI BclI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BsrBI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
HindIII HpaI Ksp632I KspI MfeI
MluI MroI MunI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TfiI Tth111I Van91I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 503 GGTACC G^GTACC
AccI 1345 GTAGAC GT^[AC][GT]AC
AccII 293 CGCG CG^CG
AcyI 826 GGCGCC G[GA]^CG[CT]C
AflIII 1384 ACATGT A^C[GA][CT]GT
AlwI 370 GGATC GGATC(4/5)
AlwNI 1022 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 555 AGCGCT AGC^GCT
ApaI 978 GGGCCC GGGCC^C
Asp718I 503 GGTACC G^GTACC
AvaI 277 CTCGGG C^[CT]CG[GA]G
BbeI 826 GGCGCC GGCGC^C
BcgI 196 CGACAAGATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 48 AGATCT A^GATCT
BsaBI 4 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 826 GGCGCC G[GA]^CG[CT]C
BseRI 1618 CTCCTC GAGGAG(10/8)
BsoBI 277 CTCGGG C^[CT]CG[GA]G
BspLU11I 1384 ACATGT A^CATGT
BsrDI 735 CATTGC GCAATG(2/0)
BssSI 1621 CTCGTG CACGAG(-5/-1)
BstUI 293 CGCG CG^CG
DrdI 245 GACCTTCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 555 AGCGCT AGC^GCT
EcoNI 893 CCTCTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1001 CCATGG C^C[AT][AT]GG
FseI 1611 GGCCGGCC GGCCGG^CC
HgaI 957 GCGTC GACGC(5/10)
Hin1I 826 GGCGCC G[GA]^CG[CT]C
HincII 1409 GTCAAC GT[CT]^[GA]AC
HindII 1409 GTCAAC GT[CT]^[GA]AC
HphI 624 TCACC GGTGA(8/7)
KasI 826 GGCGCC G^GCGCC
KpnI 503 GGTACC GGTAC^C
MaeII 387 ACGT A^CGT
MamI 4 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 467 CATGTGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 68 CAGCTG C[AC]G^C[GT]G
MvnI 293 CGCG CG^CG
NarI 826 GGCGCC GG^CGCC
NcoI 1001 CCATGG C^CATGG
NheI 772 GCTAGC G^CTAGC
NspI 1384 ACATGT [GA]CATG^[CT]
PstI 632 CTGCAG CTGCA^G
PvuII 68 CAGCTG CAG^CTG
SfoI 826 GGCGCC GGC^GCC
StyI 1001 CCATGG C^C[AT][AT]GG
TaqI 195 TCGA T^CGA
TthHB8I 195 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Alw44I 1107 GTGCAC G^TGCAC
1259 GTGCAC
ApaLI 1107 GTGCAC G^TGCAC
1259 GTGCAC
Asp700I 242 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
481 GAACGCCTTC
AviII 1046 TGCGCA TGC^GCA
1463 TGCGCA
BalI 1097 TGGCCA TGG^CCA
1195 TGGCCA
BanI 503 GGTACC G^G[CT][GA]CC
826 GGCGCC
BbsI 180 GAAGAC GAAGAC(2/6)
242 GAAGAC
BciVI 707 GGATAC GTATCC(6/5)
58 GTATCC
BpuAI 180 GAAGAC GAAGAC(2/6)
242 GAAGAC
BsaI 459 GAGACC GGTCTC(1/5)
1181 GAGACC
BstAPI 643 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1645 GCACATGGTGC
BstYI 48 AGATCT [GA]^GATC[CT]
370 GGATCT
DpnII 49 GATC ^GATC
371 GATC
DraIII 872 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
1646 CACATGGTG
FauI 291 CCCGC CCCGC(4/6)
717 CCCGC
FspI 1046 TGCGCA TGC^GCA
1463 TGCGCA
HaeII 555 AGCGCT [GA]GCGC^[CT]
826 GGCGCC
MboI 49 GATC ^GATC
371 GATC
MflI 48 AGATCT [GA]^GATC[CT]
370 GGATCT
MluNI 1097 TGGCCA TGG^CCA
1195 TGGCCA
MscI 1097 TGGCCA TGG^CCA
1195 TGGCCA
NdeII 49 GATC ^GATC
371 GATC
SacI 217 GAGCTC GAGCT^C
259 GAGCTC
Sau3AI 49 GATC ^GATC
371 GATC
SmlI 83 CTCAAG C^T[CT][GA]AG
306 CTCAAG
StuI 567 AGGCCT AGG^CCT
901 AGGCCT
XcmI 141 CCATGAAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
794 CCATCTAACTTCTGG
XhoII 48 AGATCT [GA]^GATC[CT]
370 GGATCT
XmnI 242 GAAGACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
481 GAACGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanII BbvI BcnI BfaI BmyI
BpmI BsaJI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DraII DsaI EaeI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeI
MaeIII MboII MnlI MseI MspI
MvaI MwoI NaeI NciI NgoMIV
NlaIII NlaIV PleI RsaI Sau96I
ScrFI SfaNI SfcI Tru9I TseI
Tsp45I Tsp509I TspRI