##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AatII AocI Aor51HI AscI AseI
AsnI BbeI BcgI BclI BglII
BlpI Bpu1102I BsiWI Bsp1407I BspHI
BsrGI BssHII BssSI Bsu36I CelII
CpoI DraIII Eco47III Eco81I EcoNI
FbaI FseI HindIII HpaI KasI
KspI MluI NarI NheI NotI
NruI PacI PaeR7I PmeI PshBI
PvuI RcaI RsrII SacII SbfI
SexAI SfiI SfoI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AclI 4527 AACGTT AA^CGTT
AcyI 629 GACGCC G[GA]^CG[CT]C
AgeI 4993 ACCGGT A^CCGGT
AspI 251 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 317 CACGTG CAC^GTG
BsaHI 629 GACGCC G[GA]^CG[CT]C
BsaI 1144 GGTCTC GGTCTC(1/5)
BsmI 1974 GCATTC GAATGC(1/-1)
BspDI 2690 ATCGAT AT^CGAT
BspLU11I 576 ACATGT A^CATGT
BsrBI 1030 GAGCGG CCGCTC(-3/-3)
Bst1107I 861 GTATAC GTA^TAC
BstBI 658 TTCGAA TT^CGAA
BstZ17I 861 GTATAC GTA^TAC
ClaI 2690 ATCGAT AT^CGAT
DraI 5467 TTTAAA TTT^AAA
DrdI 932 GACATTCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4424 GAATTC G^AATTC
EcoRV 1154 GATATC GAT^ATC
EcoT22I 3482 ATGCAT ATGCA^T
Hin1I 629 GACGCC G[GA]^CG[CT]C
NaeI 725 GCCGGC GCC^GGC
NdeI 1725 CATATG CA^TATG
NgoMIV 725 GCCGGC G^CCGGC
NsiI 3482 ATGCAT ATGCA^T
NspV 658 TTCGAA TT^CGAA
PflFI 251 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 4993 ACCGGT A^CCGGT
PmaCI 317 CACGTG CAC^GTG
PmlI 317 CACGTG CAC^GTG
Psp1406I 4527 AACGTT AA^CGTT
SacI 3973 GAGCTC GAGCT^C
SalI 2703 GTCGAC G^TCGAC
SfuI 658 TTCGAA TT^CGAA
Tth111I 251 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2368 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 1180 GGTACC G^GTACC
2409 GGTACC
AccIII 1544 TCCGGA T^CCGGA
3602 TCCGGA
AflII 2240 CTTAAG C^TTAAG
4460 CTTAAG
Alw44I 2090 GTGCAC G^TGCAC
3079 GTGCAC
ApaI 3758 GGGCCC GGGCC^C
3930 GGGCCC
ApaLI 2090 GTGCAC G^TGCAC
3079 GTGCAC
Asp700I 1102 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4619 GAAACAATTC
Asp718I 1180 GGTACC G^GTACC
2409 GGTACC
AviII 276 TGCGCA TGC^GCA
3445 TGCGCA
AvrII 1588 CCTAGG C^CTAGG
2500 CCTAGG
BanI 1180 GGTACC G^G[CT][GA]CC
2409 GGTACC
BbvCI 2781 CCTCAGC CCTCAGC(-5/-2)
2602 GCTGAGG
BciVI 394 GGATAC GTATCC(6/5)
3157 GTATCC
BfrI 2240 CTTAAG C^TTAAG
4460 CTTAAG
BlnI 1588 CCTAGG C^CTAGG
2500 CCTAGG
BsaAI 317 CACGTG [CT]AC^GT[GA]
3477 CACGTA
BsaBI 208 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2558 GATGCACATC
BseAI 1544 TCCGGA T^CCGGA
3602 TCCGGA
BsiEI 1363 CGGCCG CG[GA][CT]^CG
2302 CGGCCG
BsmBI 3979 CGTCTC CGTCTC(1/5)
3608 GAGACG
BspEI 1544 TCCGGA T^CCGGA
3602 TCCGGA
BstAPI 1080 GCAAGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1349 GCAGTGGATGC
BstEII 436 GGTCACC G^GT[ACGT]ACC
3429 GGTGACC
BstPI 436 GGTCACC G^GT[ACGT]ACC
3429 GGTGACC
EagI 1363 CGGCCG C^GGCCG
2302 CGGCCG
EclXI 1363 CGGCCG C^GGCCG
2302 CGGCCG
Eco52I 1363 CGGCCG C^GGCCG
2302 CGGCCG
EcoO65I 436 GGTCACC G^GT[ACGT]ACC
3429 GGTGACC
FspI 276 TGCGCA TGC^GCA
3445 TGCGCA
HaeII 39 AGCGCC [GA]GCGC^[CT]
1685 GGCGCT
HincII 1865 GTTGAC GT[CT]^[GA]AC
2703 GTCGAC
HindII 1865 GTTGAC GT[CT]^[GA]AC
2703 GTCGAC
KpnI 1180 GGTACC GGTAC^C
2409 GGTACC
MamI 208 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2558 GATGCACATC
MfeI 426 CAATTG C^AATTG
5392 CAATTG
MroI 1544 TCCGGA T^CCGGA
3602 TCCGGA
MunI 426 CAATTG C^AATTG
5392 CAATTG
NcoI 564 CCATGG C^CATGG
4404 CCATGG
NspI 576 ACATGT [GA]CATG^[CT]
1085 ACATGC
StuI 956 AGGCCT AGG^CCT
1843 AGGCCT
XmnI 1102 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4619 GAAACAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AhdI AluI AlwI AlwNI
ApoI AspEI AspHI AvaI AvaII
BalI BamHI BanII BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eam1105I EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI PshAI PstI PvuII
RsaI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI