##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AccII AclI AgeI AscI BbeI
BbrPI BcgI BciVI BclI BsaAI
BsiWI BspDI BsrBI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I ClaI CpoI EagI EclXI
Eco52I EcoO65I FbaI FseI KasI
KspI MluI MvnI NaeI NarI
NdeI NgoMIV NotI NruI NspV
PacI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacII SalI
SbfI SfoI SfuI SmaI SnaBI
Sse8387I SspI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccIII 1052 TCCGGA T^CCGGA
Aor51HI 2284 AGCGCT AGC^GCT
AseI 4045 ATTAAT AT^TAAT
AsnI 4045 ATTAAT AT^TAAT
AviII 2243 TGCGCA TGC^GCA
BamHI 952 GGATCC G^GATCC
BbvCI 5641 GCTGAGG CCTCAGC(-5/-2)
BsaBI 1290 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1052 TCCGGA [AT]^CCGG[AT]
BseAI 1052 TCCGGA T^CCGGA
BsiEI 2229 CGACCG CG[GA][CT]^CG
BsmBI 3671 GAGACG CGTCTC(1/5)
BspEI 1052 TCCGGA T^CCGGA
BstAPI 2510 GCATTCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DrdI 2788 GACAGGGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2284 AGCGCT AGC^GCT
EcoRI 5037 GAATTC G^AATTC
EcoRV 385 GATATC GAT^ATC
FspI 2243 TGCGCA TGC^GCA
HaeII 2284 AGCGCT [GA]GCGC^[CT]
HpaI 5714 GTTAAC GTT^AAC
MamI 1290 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4302 CAATTG C^AATTG
MroI 1052 TCCGGA T^CCGGA
MunI 4302 CAATTG C^AATTG
NcoI 3453 CCATGG C^CATGG
PaeR7I 5310 CTCGAG C^TCGAG
PshAI 3846 GACTTAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4045 ATTAAT AT^TAAT
SexAI 3764 ACCTGGT A^CC[AT]GGT
SfiI 2091 GGCCATGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 1481 CGCCGGTG C[GA]^CCGG[CT]G
SpeI 4095 ACTAGT A^CTAGT
SphI 2114 GCATGC GCATG^C
XhoI 5310 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AatII 1123 GACGTC GACGT^C
3673 GACGTC
Acc65I 565 GGTACC G^GTACC
2343 GGTACC
AcyI 1123 GACGTC G[GA]^CG[CT]C
3673 GACGTC
AflII 960 CTTAAG C^TTAAG
4377 CTTAAG
AhdI 1043 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2365 GACCTGCAGTC
AocI 322 CCTGAGG CC^T[ACGT]AGG
3612 CCTCAGG
Asp718I 565 GGTACC G^GTACC
2343 GGTACC
AspEI 1043 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2365 GACCTGCAGTC
AvrII 2013 CCTAGG C^CTAGG
2347 CCTAGG
BfrI 960 CTTAAG C^TTAAG
4377 CTTAAG
BglII 803 AGATCT A^GATCT
5591 AGATCT
BlnI 2013 CCTAGG C^CTAGG
2347 CCTAGG
BlpI 2059 GCTGAGC GC^T[ACGT]AGC
4919 GCTGAGC
Bpu1102I 2059 GCTGAGC GC^T[ACGT]AGC
4919 GCTGAGC
BsaHI 1123 GACGTC G[GA]^CG[CT]C
3673 GACGTC
Bsp1407I 3702 TGTACA T^GTACA
4541 TGTACA
BsrFI 1482 GCCGGT [GA]^CCGG[CT]
4353 ACCGGC
BsrGI 3702 TGTACA T^GTACA
4541 TGTACA
Bsu36I 322 CCTGAGG CC^T[ACGT]AGG
3612 CCTCAGG
CelII 2059 GCTGAGC GC^T[ACGT]AGC
4919 GCTGAGC
Cfr10I 1482 GCCGGT [GA]^CCGG[CT]
4353 ACCGGC
DraI 1168 TTTAAA TTT^AAA
4280 TTTAAA
Eam1105I 1043 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2365 GACCTGCAGTC
Eco81I 322 CCTGAGG CC^T[ACGT]AGG
3612 CCTCAGG
EcoT22I 1006 ATGCAT ATGCA^T
4291 ATGCAT
HgaI 3552 GACGC GACGC(5/10)
5209 GCGTC
Hin1I 1123 GACGTC G[GA]^CG[CT]C
3673 GACGTC
KpnI 565 GGTACC GGTAC^C
2343 GGTACC
NheI 999 GCTAGC G^CTAGC
3528 GCTAGC
NsiI 1006 ATGCAT ATGCA^T
4291 ATGCAT
PflMI 4012 CCATCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4136 CCACATGGTGG
PpuMI 2888 AGGACCT [GA]G^G[AT]CC[CT]
3596 AGGACCC
SacI 2221 GAGCTC GAGCT^C
2380 GAGCTC
SapI 1645 GAAGAGC GCTCTTC(1/4)
3637 GAAGAGC
ScaI 4506 AGTACT AGT^ACT
5119 AGTACT
SspBI 3702 TGTACA T^GTACA
4541 TGTACA
TaqI 5277 TCGA T^CGA
5311 TCGA
TthHB8I 5277 TCGA T^CGA
5311 TCGA
Van91I 4012 CCATCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4136 CCACATGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AciI AcsI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaI
ApaLI ApoI Asp700I AspHI AspI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspHI BspLU11I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PleI PstI PvuII RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
XbaI XcmI XhoII XmnI