##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
AatII Acc65I AclI AflIII AgeI
AscI AseI AsnI Asp718I BbrPI
BbsI BclI BpuAI BsaAI BsaBI
BsiWI BsmI BspDI BspLU11I BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
EagI EclXI Eco52I EcoT22I FbaI
FseI HpaI KpnI KspI MamI
MluI NdeI NotI NruI NsiI
NspI NspV PacI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SacII SalI ScaI SfuI
SgrAI SnaBI SphI SspI SwaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AccII 3865 CGCG CG^CG
AocI 2370 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1613 AGCGCT AGC^GCT
AvrII 5081 CCTAGG C^CTAGG
BamHI 591 GGATCC G^GATCC
BglII 2801 AGATCT A^GATCT
BlnI 5081 CCTAGG C^CTAGG
BsiEI 2291 CGACCG CG[GA][CT]^CG
BspHI 5254 TCATGA T^CATGA
BsrBI 700 GAGCGG CCGCTC(-3/-3)
BsrFI 886 GCCGGC [GA]^CCGG[CT]
BssSI 65 CACGAG CACGAG(-5/-1)
BstUI 3865 CGCG CG^CG
Bsu36I 2370 CCTGAGG CC^T[ACGT]AGG
Cfr10I 886 GCCGGC [GA]^CCGG[CT]
DraI 3051 TTTAAA TTT^AAA
Eco47III 1613 AGCGCT AGC^GCT
Eco81I 2370 CCTGAGG CC^T[ACGT]AGG
EcoRI 5360 GAATTC G^AATTC
HincII 3925 GTTGAC GT[CT]^[GA]AC
HindII 3925 GTTGAC GT[CT]^[GA]AC
HindIII 2675 AAGCTT A^AGCTT
MfeI 5171 CAATTG C^AATTG
MunI 5171 CAATTG C^AATTG
MvnI 3865 CGCG CG^CG
NaeI 886 GCCGGC GCC^GGC
NgoMIV 886 GCCGGC G^CCGGC
RcaI 5254 TCATGA T^CATGA
SbfI 3916 CCTGCAGG CCTGCA^GG
SfiI 2633 GGCCTCCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 3916 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccI 1515 GTAGAC GT^[AC][GT]AC
5442 GTCTAC
AcsI 1872 GAATTT [GA]^AATT[CT]
5360 GAATTC
AflII 2940 CTTAAG C^TTAAG
3316 CTTAAG
AhdI 4193 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4868 GACTCCATGTC
ApoI 1872 GAATTT [GA]^AATT[CT]
5360 GAATTC
Asp700I 2593 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2672 GAAAAGCTTC
AspEI 4193 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4868 GACTCCATGTC
AspI 527 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1382 GACACAGTC
AviII 2650 TGCGCA TGC^GCA
4217 TGCGCA
BbeI 1897 GGCGCC GGCGC^C
3845 GGCGCC
BbvCI 881 CCTCAGC CCTCAGC(-5/-2)
182 GCTGAGG
BcgI 2731 GCACCCACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5370 GCAAACCCATCG
BciVI 3377 GTATCC GTATCC(6/5)
4497 GTATCC
BfrI 2940 CTTAAG C^TTAAG
3316 CTTAAG
BlpI 767 GCTGAGC GC^T[ACGT]AGC
1365 GCTCAGC
Bpu1102I 767 GCTGAGC GC^T[ACGT]AGC
1365 GCTCAGC
Bsp1407I 914 TGTACA T^GTACA
4457 TGTACA
BsrGI 914 TGTACA T^GTACA
4457 TGTACA
BstAPI 2018 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3385 GCAGAGGGTGC
BstEII 1129 GGTAACC G^GT[ACGT]ACC
1484 GGTGACC
BstPI 1129 GGTAACC G^GT[ACGT]ACC
1484 GGTGACC
CelII 767 GCTGAGC GC^T[ACGT]AGC
1365 GCTCAGC
Eam1105I 4193 GACAGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4868 GACTCCATGTC
EcoO65I 1129 GGTAACC G^GT[ACGT]ACC
1484 GGTGACC
EcoRV 3153 GATATC GAT^ATC
4171 GATATC
FspI 2650 TGCGCA TGC^GCA
4217 TGCGCA
KasI 1897 GGCGCC G^GCGCC
3845 GGCGCC
NarI 1897 GGCGCC GG^CGCC
3845 GGCGCC
NheI 1565 GCTAGC G^CTAGC
3670 GCTAGC
PflFI 527 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1382 GACACAGTC
SexAI 1750 ACCTGGT A^CC[AT]GGT
2157 ACCTGGT
SfoI 1897 GGCGCC GGC^GCC
3845 GGCGCC
SmaI 905 CCCGGG CCC^GGG
1480 CCCGGG
SpeI 833 ACTAGT A^CTAGT
5115 ACTAGT
SspBI 914 TGTACA T^GTACA
4457 TGTACA
Tth111I 527 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
1382 GACACAGTC
XmaCI 905 CCCGGG C^CCGGG
1480 CCCGGG
XmaI 905 CCCGGG C^CCGGG
1480 CCCGGG
XmnI 2593 GAACTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2672 GAAAAGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccIII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI ApaI ApaLI
AspHI AvaI AvaII BalI BanI
BanII BbvI BcnI BfaI BglI
BmyI BpmI BsaHI BsaI BsaJI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspEI BspMI BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DraIII DrdI DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MroI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV PaeR7I PflMI PleI
PpuMI PshAI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XbaI
XcmI XhoI XhoII