##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I Alw44I ApaI ApaLI AscI
Asp718I AviII BamHI BanI BbeI
BclI BlpI Bpu1102I BsiWI BspDI
BspLU11I BsrBI BssHII Bst1107I BstEII
BstPI BstZ17I CelII ClaI CpoI
DrdI EcoO65I EcoT22I FbaI FseI
FspI KasI KpnI KspI MluI
NarI NcoI NotI NruI NsiI
PacI PaeR7I PmeI PvuI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SnaBI SpeI Sse8387I StuI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 999 GTAGAC GT^[AC][GT]AC
AccIII 4260 TCCGGA T^CCGGA
AclI 933 AACGTT AA^CGTT
AflII 3140 CTTAAG C^TTAAG
AflIII 995 ACACGT A^C[GA][CT]GT
AhdI 2187 GACACTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1033 AGCGCT AGC^GCT
Asp700I 2579 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2187 GACACTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 4895 CCTAGG C^CTAGG
BalI 4695 TGGCCA TGG^CCA
BbrPI 4093 CACGTG CAC^GTG
BciVI 354 GTATCC GTATCC(6/5)
BfrI 3140 CTTAAG C^TTAAG
BglI 1903 GCCCCGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4895 CCTAGG C^CTAGG
BsaBI 159 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 4260 TCCGGA T^CCGGA
BsmI 4964 GCATTC GAATGC(1/-1)
Bsp1407I 528 TGTACA T^GTACA
BspEI 4260 TCCGGA T^CCGGA
BspHI 4608 TCATGA T^CATGA
BsrGI 528 TGTACA T^GTACA
BssSI 2796 CTCGTG CACGAG(-5/-1)
BstBI 1243 TTCGAA TT^CGAA
BstXI 313 CCAGCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 103 CGGCCG C^GGCCG
Eam1105I 2187 GACACTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 103 CGGCCG C^GGCCG
Eco47III 1033 AGCGCT AGC^GCT
Eco52I 103 CGGCCG C^GGCCG
EcoRV 3347 GATATC GAT^ATC
MamI 159 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 4695 TGGCCA TGG^CCA
MroI 4260 TCCGGA T^CCGGA
MscI 4695 TGGCCA TGG^CCA
NaeI 101 GCCGGC GCC^GGC
NdeI 1143 CATATG CA^TATG
NgoMIV 101 GCCGGC G^CCGGC
NspV 1243 TTCGAA TT^CGAA
PmaCI 4093 CACGTG CAC^GTG
PmlI 4093 CACGTG CAC^GTG
Psp1406I 933 AACGTT AA^CGTT
RcaI 4608 TCATGA T^CATGA
SapI 86 GAAGAGC GCTCTTC(1/4)
ScaI 4742 AGTACT AGT^ACT
SfuI 1243 TTCGAA TT^CGAA
SgrAI 2560 CACCGGTG C[GA]^CCGG[CT]G
SspBI 528 TGTACA T^GTACA
XbaI 4558 TCTAGA T^CTAGA
XmnI 2579 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccII 2252 CGCG CG^CG
3034 CGCG
AgeI 2339 ACCGGT A^CCGGT
2561 ACCGGT
AspI 3570 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4551 GACTCTGTC
BbsI 129 GAAGAC GAAGAC(2/6)
3003 GAAGAC
BbvCI 4351 CCTCAGC CCTCAGC(-5/-2)
2327 GCTGAGG
BcgI 339 CGAAAGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1593 CGAGTGGTCTGC
BpuAI 129 GAAGAC GAAGAC(2/6)
3003 GAAGAC
BsiEI 103 CGGCCG CG[GA][CT]^CG
1589 CGGTCG
BstUI 2252 CGCG CG^CG
3034 CGCG
DraIII 652 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
2061 CACTTTGTG
EaeI 103 CGGCCG [CT]^GGCC[GA]
4695 TGGCCA
EarI 2888 CTCTTC CTCTTC(1/4)
86 GAAGAG
EcoNI 1502 CCTGCTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2409 CCTGTGGCAGG
HindIII 1998 AAGCTT A^AGCTT
5330 AAGCTT
Ksp632I 2888 CTCTTC CTCTTC(1/4)
86 GAAGAG
MfeI 4037 CAATTG C^AATTG
5287 CAATTG
MunI 4037 CAATTG C^AATTG
5287 CAATTG
MvnI 2252 CGCG CG^CG
3034 CGCG
NheI 456 GCTAGC G^CTAGC
3194 GCTAGC
PflFI 3570 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4551 GACTCTGTC
PinAI 2339 ACCGGT A^CCGGT
2561 ACCGGT
PshAI 688 GACTGGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1656 GACCTGTGTC
SacI 3210 GAGCTC GAGCT^C
4264 GAGCTC
SphI 1772 GCATGC GCATG^C
2791 GCATGC
SspI 3870 AATATT AAT^ATT
5337 AATATT
Tth111I 3570 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4551 GACTCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AatII AciI AcsI AcyI AfaI
AluI AlwI AlwNI AocI ApoI
AseI AsnI AspHI AvaI AvaII
BanII BbvI BcnI BfaI BglII
BmyI BpmI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI Eco81I EcoO109I EcoRI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PshBI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI