##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccIII AclI AgeI ApaI
AscI AseI AsnI AspI AvrII
BamHI BbeI BciVI BclI BlnI
BsaBI BseAI BsiWI BspEI BspHI
BspLU11I BsrFI BssSI Bst1107I BstZ17I
Cfr10I CpoI EagI EclXI Eco52I
EcoRI EcoRV FbaI FseI KasI
MamI MfeI MluI MroI MunI
NaeI NarI NgoMIV NotI NruI
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I RcaI RsrII SalI
SbfI SfiI SfoI SgrAI SpeI
Sse8387I SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 3050 GGTACC G^GTACC
AflII 4650 CTTAAG C^TTAAG
AflIII 1169 ACACGT A^C[GA][CT]GT
AhdI 1631 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3113 CCTCAGG CC^T[ACGT]AGG
Asp718I 3050 GGTACC G^GTACC
AspEI 1631 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BcgI 520 GCAGGAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4650 CTTAAG C^TTAAG
BlpI 4007 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4007 GCTGAGC GC^T[ACGT]AGC
BsaWI 1601 TCCGGT [AT]^CCGG[AT]
BsiEI 1954 CGATCG CG[GA][CT]^CG
BsmI 3150 GCATTC GAATGC(1/-1)
Bsp1407I 4969 TGTACA T^GTACA
BspDI 4095 ATCGAT AT^CGAT
BsrGI 4969 TGTACA T^GTACA
BstBI 787 TTCGAA TT^CGAA
BstEII 1803 GGTGACC G^GT[ACGT]ACC
BstPI 1803 GGTGACC G^GT[ACGT]ACC
Bsu36I 3113 CCTCAGG CC^T[ACGT]AGG
CelII 4007 GCTGAGC GC^T[ACGT]AGC
ClaI 4095 ATCGAT AT^CGAT
DrdI 5375 GACTTTCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1631 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3113 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1803 GGTGACC G^GT[ACGT]ACC
EcoT22I 5363 ATGCAT ATGCA^T
KpnI 3050 GGTACC GGTAC^C
KspI 2665 CCGCGG CCGC^GG
NcoI 2434 CCATGG C^CATGG
NdeI 1861 CATATG CA^TATG
NsiI 5363 ATGCAT ATGCA^T
NspV 787 TTCGAA TT^CGAA
PshAI 60 GACCCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1954 CGATCG CGAT^CG
SacII 2665 CCGCGG CCGC^GG
SexAI 4026 ACCAGGT A^CC[AT]GGT
SfuI 787 TTCGAA TT^CGAA
SnaBI 1339 TACGTA TAC^GTA
SphI 5098 GCATGC GCATG^C
SspBI 4969 TGTACA T^GTACA
SspI 5203 AATATT AAT^ATT
StuI 4347 AGGCCT AGG^CCT
XbaI 1489 TCTAGA T^CTAGA
XcmI 3947 CCATGTGGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Alw44I 2844 GTGCAC G^TGCAC
5478 GTGCAC
Aor51HI 363 AGCGCT AGC^GCT
1010 AGCGCT
ApaLI 2844 GTGCAC G^TGCAC
5478 GTGCAC
Asp700I 5012 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5341 GAATTGGTTC
BbrPI 1170 CACGTG CAC^GTG
4104 CACGTG
BbsI 4230 GAAGAC GAAGAC(2/6)
3891 GTCTTC
BglI 1301 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3223 GCCCAGAGGGC
BpuAI 4230 GAAGAC GAAGAC(2/6)
3891 GTCTTC
BsaI 58 GAGACC GGTCTC(1/5)
3404 GAGACC
BsmBI 100 GAGACG CGTCTC(1/5)
1120 GAGACG
BsrBI 167 GAGCGG CCGCTC(-3/-3)
344 GAGCGG
BssHII 2556 GCGCGC G^CGCGC
2748 GCGCGC
BstAPI 3988 GCAGTCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5098 GCATGCACTGC
DsaI 2434 CCATGG C^C[GA][CT]GG
2665 CCGCGG
Eco47III 363 AGCGCT AGC^GCT
1010 AGCGCT
HpaI 1904 GTTAAC GTT^AAC
4664 GTTAAC
NheI 2552 GCTAGC G^CTAGC
4851 GCTAGC
PmaCI 1170 CACGTG CAC^GTG
4104 CACGTG
PmlI 1170 CACGTG CAC^GTG
4104 CACGTG
ScaI 2135 AGTACT AGT^ACT
4211 AGTACT
SmaI 62 CCCGGG CCC^GGG
1204 CCCGGG
XmaCI 62 CCCGGG C^CCGGG
1204 CCCGGG
XmaI 62 CCCGGG C^CCGGG
1204 CCCGGG
XmnI 5012 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5341 GAATTGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI ApoI
AspHI AvaI AvaII AviII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BglII BmyI BpmI BsaAI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DraIII EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII