##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
Acc65I AccIII AclI AgeI AhdI
AscI Asp718I AspEI AspI AvrII
BamHI BbvCI BcgI BglI BlnI
BsaI BseAI BsiWI BspDI BspEI
BspHI BstAPI ClaI DrdI Eam1105I
EcoRV FseI HpaI KpnI MluI
MroI NotI NruI PacI PaeR7I
PflFI PinAI PmeI PshAI Psp1406I
PvuI RcaI SalI SbfI SexAI
SfiI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Alw44I 144 GTGCAC G^TGCAC
AlwI 3050 GGATC GGATC(4/5)
AocI 3613 CCTTAGG CC^T[ACGT]AGG
ApaI 282 GGGCCC GGGCC^C
ApaLI 144 GTGCAC G^TGCAC
Asp700I 2270 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1679 TGCGCA TGC^GCA
BbrPI 2264 CACGTG CAC^GTG
BciVI 2287 GTATCC GTATCC(6/5)
BlpI 5218 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 5218 GCTAAGC GC^T[ACGT]AGC
BsaBI 1027 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 2378 GAGACG CGTCTC(1/5)
BsrBI 1473 GAGCGG CCGCTC(-3/-3)
BsrDI 3473 GCAATG GCAATG(2/0)
BssHII 408 GCGCGC G^CGCGC
Bst1107I 4153 GTATAC GTA^TAC
BstBI 2091 TTCGAA TT^CGAA
BstEII 818 GGTCACC G^GT[ACGT]ACC
BstPI 818 GGTCACC G^GT[ACGT]ACC
BstZ17I 4153 GTATAC GTA^TAC
Bsu36I 3613 CCTTAGG CC^T[ACGT]AGG
CelII 5218 GCTAAGC GC^T[ACGT]AGC
CpoI 107 CGGTCCG CG^G[AT]CCG
EagI 220 CGGCCG C^GGCCG
EclXI 220 CGGCCG C^GGCCG
Eco52I 220 CGGCCG C^GGCCG
Eco81I 3613 CCTTAGG CC^T[ACGT]AGG
EcoO65I 818 GGTCACC G^GT[ACGT]ACC
EcoT22I 3476 ATGCAT ATGCA^T
FspI 1679 TGCGCA TGC^GCA
MamI 1027 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 3476 ATGCAT ATGCA^T
NspV 2091 TTCGAA TT^CGAA
PmaCI 2264 CACGTG CAC^GTG
PmlI 2264 CACGTG CAC^GTG
RsrII 107 CGGTCCG CG^G[AT]CCG
SapI 5318 GCTCTTC GCTCTTC(1/4)
SfuI 2091 TTCGAA TT^CGAA
StuI 544 AGGCCT AGG^CCT
XmnI 2270 GAATGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 431 GACGTC GACGT^C
2380 GACGTC
AccI 1207 GTAGAC GT^[AC][GT]AC
4153 GTATAC
Aor51HI 499 AGCGCT AGC^GCT
1484 AGCGCT
AseI 3754 ATTAAT AT^TAAT
4771 ATTAAT
AsnI 3754 ATTAAT AT^TAAT
4771 ATTAAT
BalI 439 TGGCCA TGG^CCA
3112 TGGCCA
BsaWI 712 TCCGGT [AT]^CCGG[AT]
2039 ACCGGA
BsiEI 220 CGGCCG CG[GA][CT]^CG
485 CGACCG
BssSI 3 CTCGTG CACGAG(-5/-1)
75 CTCGTG
BstXI 1002 CCAAGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3376 CCACCATACTGG
DraIII 416 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
576 CACATGGTG
Eco47III 499 AGCGCT AGC^GCT
1484 AGCGCT
EcoNI 536 CCTAGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1549 CCTTACTTAGG
EcoRI 2274 GAATTC G^AATTC
4413 GAATTC
HindIII 3321 AAGCTT A^AGCTT
4287 AAGCTT
KspI 137 CCGCGG CCGC^GG
350 CCGCGG
MfeI 1197 CAATTG C^AATTG
4117 CAATTG
MluNI 439 TGGCCA TGG^CCA
3112 TGGCCA
MscI 439 TGGCCA TGG^CCA
3112 TGGCCA
MunI 1197 CAATTG C^AATTG
4117 CAATTG
NaeI 34 GCCGGC GCC^GGC
319 GCCGGC
NcoI 3277 CCATGG C^CATGG
4248 CCATGG
NdeI 3784 CATATG CA^TATG
4144 CATATG
NgoMIV 34 GCCGGC G^CCGGC
319 GCCGGC
PshBI 3754 ATTAAT AT^TAAT
4771 ATTAAT
SacI 72 GAGCTC GAGCT^C
685 GAGCTC
SacII 137 CCGCGG CCGC^GG
350 CCGCGG
XbaI 1490 TCTAGA T^CTAGA
3987 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflII AflIII AluI AlwNI ApoI
AspHI AvaI AvaII BanI BanII
BbeI BbsI BbvI BclI BcnI
BfaI BfrI BglII BmyI BpmI
BpuAI BsaAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspLU11I BspMI BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI KasI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NheI NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScaI ScrFI SfaNI
SfcI SfoI SmlI SspBI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII