##################################
# NO CUT ENZYMES ( 60 enzymes) #
##################################
Acc65I AccI AccIII AgeI AscI
AseI AsnI Asp718I AviII BsaBI
BseAI BsiWI BspDI BspEI BspLU11I
BssHII BssSI Bst1107I BstZ17I ClaI
CpoI EcoT22I FseI FspI KpnI
KspI MamI MfeI MluI MroI
MunI NheI NotI NruI NsiI
PacI PaeR7I PinAI PmeI PshAI
PshBI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SgrAI
SmaI SnaBI SpeI Sse8387I SspI
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 138 GACGTC GACGT^C
AccII 248 CGCG CG^CG
AclI 3154 AACGTT AA^CGTT
AflII 1929 CTTAAG C^TTAAG
Alw44I 4322 GTGCAC G^TGCAC
ApaLI 4322 GTGCAC G^TGCAC
AvrII 3502 CCTAGG C^CTAGG
BalI 3744 TGGCCA TGG^CCA
BamHI 4203 GGATCC G^GATCC
BanI 4780 GGCGCC G^G[CT][GA]CC
BbeI 4780 GGCGCC GGCGC^C
BcgI 1052 CGAATTCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 449 GTATCC GTATCC(6/5)
BclI 117 TGATCA T^GATCA
BfrI 1929 CTTAAG C^TTAAG
BglII 3283 AGATCT A^GATCT
BlnI 3502 CCTAGG C^CTAGG
BsaI 3806 GGTCTC GGTCTC(1/5)
Bsp1407I 918 TGTACA T^GTACA
BsrBI 3872 GAGCGG CCGCTC(-3/-3)
BsrGI 918 TGTACA T^GTACA
BstEII 1198 GGTTACC G^GT[ACGT]ACC
BstPI 1198 GGTTACC G^GT[ACGT]ACC
BstUI 248 CGCG CG^CG
DrdI 1506 GACGTACAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 4538 CGGCCG C^GGCCG
EclXI 4538 CGGCCG C^GGCCG
Eco52I 4538 CGGCCG C^GGCCG
EcoNI 3047 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1198 GGTTACC G^GT[ACGT]ACC
EcoRV 2681 GATATC GAT^ATC
FbaI 117 TGATCA T^GATCA
HgaI 3241 GACGC GACGC(5/10)
HincII 2742 GTTAAC GT[CT]^[GA]AC
HindII 2742 GTTAAC GT[CT]^[GA]AC
HindIII 182 AAGCTT A^AGCTT
HpaI 2742 GTTAAC GTT^AAC
KasI 4780 GGCGCC G^GCGCC
MluNI 3744 TGGCCA TGG^CCA
MscI 3744 TGGCCA TGG^CCA
MvnI 248 CGCG CG^CG
NaeI 4536 GCCGGC GCC^GGC
NarI 4780 GGCGCC GG^CGCC
NdeI 4706 CATATG CA^TATG
NgoMIV 4536 GCCGGC G^CCGGC
PpuMI 241 AGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 3154 AACGTT AA^CGTT
SfoI 4780 GGCGCC GGC^GCC
SphI 3366 GCATGC GCATG^C
SspBI 918 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 138 GACGTC G[GA]^CG[CT]C
4780 GGCGCC
AflIII 2227 ACGTGT A^C[GA][CT]GT
3849 ACACGT
AhdI 731 GACCCGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1603 GACAGAAGGTC
ApaI 1012 GGGCCC GGGCC^C
2634 GGGCCC
Asp700I 1049 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3317 GAATGGCTTC
AspEI 731 GACCCGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1603 GACAGAAGGTC
BbrPI 2226 CACGTG CAC^GTG
3850 CACGTG
BlpI 2220 GCTGAGC GC^T[ACGT]AGC
2254 GCTGAGC
Bpu1102I 2220 GCTGAGC GC^T[ACGT]AGC
2254 GCTGAGC
BsaAI 2226 CACGTG [CT]AC^GT[GA]
3850 CACGTG
BsaHI 138 GACGTC G[GA]^CG[CT]C
4780 GGCGCC
BsaWI 69 TCCGGT [AT]^CCGG[AT]
1202 ACCGGA
BsmI 2154 GAATGC GAATGC(1/-1)
3255 GAATGC
BspHI 1266 TCATGA T^CATGA
3453 TCATGA
BsrDI 1142 CATTGC GCAATG(2/0)
4063 CATTGC
BsrFI 355 ACCGGC [GA]^CCGG[CT]
4536 GCCGGC
BstBI 13 TTCGAA TT^CGAA
4010 TTCGAA
BstXI 1406 CCAAAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1826 CCATATATTTGG
CelII 2220 GCTGAGC GC^T[ACGT]AGC
2254 GCTGAGC
Cfr10I 355 ACCGGC [GA]^CCGG[CT]
4536 GCCGGC
DraI 4716 TTTAAA TTT^AAA
4739 TTTAAA
Eam1105I 731 GACCCGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1603 GACAGAAGGTC
Hin1I 138 GACGTC G[GA]^CG[CT]C
4780 GGCGCC
NspV 13 TTCGAA TT^CGAA
4010 TTCGAA
PmaCI 2226 CACGTG CAC^GTG
3850 CACGTG
PmlI 2226 CACGTG CAC^GTG
3850 CACGTG
RcaI 1266 TCATGA T^CATGA
3453 TCATGA
SacI 1678 GAGCTC GAGCT^C
2398 GAGCTC
SfuI 13 TTCGAA TT^CGAA
4010 TTCGAA
StuI 1570 AGGCCT AGG^CCT
3044 AGGCCT
XmnI 1049 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3317 GAATGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI Aor51HI ApoI AspHI
AspI AvaI AvaII BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI Eco47III Eco81I
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflFI PflMI PleI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII