################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## Acc65I AccIII AclI AflII AgeI AhdI ApaI AscI AseI AsnI Asp718I AspEI AviII BbeI BciVI BclI BfrI BsaBI BseAI BsiWI BspDI BspEI BspHI BspLU11I BsrBI BssHII BssSI BstBI ClaI CpoI DraI EagI Eam1105I EclXI Eco52I FbaI FseI FspI HindIII HpaI KasI KpnI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NspV PacI PinAI PmeI PshBI Psp1406I RcaI RsrII SalI ScaI SexAI SfoI SfuI SgrAI SnaBI SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AatII 1193 GACGTC GACGT^C AcsI 2778 GAATTC [GA]^AATT[CT] ApoI 2778 GAATTC [GA]^AATT[CT] AspI 471 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 2824 GGATCC G^GATCC BcgI 3410 GCAAAAGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaWI 4191 TCCGGT [AT]^CCGG[AT] BsiEI 4861 CGATCG CG[GA][CT]^CG BsmI 3963 GAATGC GAATGC(1/-1) BsrDI 463 GCAATG GCAATG(2/0) Bst1107I 3780 GTATAC GTA^TAC BstZ17I 3780 GTATAC GTA^TAC DrdI 960 GACTCGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 2778 GAATTC G^AATTC EcoRV 3674 GATATC GAT^ATC EcoT22I 753 ATGCAT ATGCA^T HincII 124 GTTGAC GT[CT]^[GA]AC HindII 124 GTTGAC GT[CT]^[GA]AC KspI 38 CCGCGG CCGC^GG NsiI 753 ATGCAT ATGCA^T PaeR7I 1533 CTCGAG C^TCGAG PflFI 471 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 1662 GACCAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuI 4861 CGATCG CGAT^CG SacII 38 CCGCGG CCGC^GG SapI 2407 GCTCTTC GCTCTTC(1/4) SfiI 818 GGCCACACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SpeI 3688 ACTAGT A^CTAGT Tth111I 471 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1142 CCAGGCCCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1533 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcyI 1193 GACGTC G[GA]^CG[CT]C 1701 GGCGTC AocI 707 CCTCAGG CC^T[ACGT]AGG 3843 CCTAAGG Aor51HI 1636 AGCGCT AGC^GCT 3745 AGCGCT Asp700I 1160 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4409 GAATGGATTC AvrII 1811 CCTAGG C^CTAGG 4053 CCTAGG BlnI 1811 CCTAGG C^CTAGG 4053 CCTAGG BsaHI 1193 GACGTC G[GA]^CG[CT]C 1701 GGCGTC Bsu36I 707 CCTCAGG CC^T[ACGT]AGG 3843 CCTAAGG Eco47III 1636 AGCGCT AGC^GCT 3745 AGCGCT Eco81I 707 CCTCAGG CC^T[ACGT]AGG 3843 CCTAAGG FauI 932 GCGGG CCCGC(4/6) 1433 GCGGG HaeII 1636 AGCGCT [GA]GCGC^[CT] 3745 AGCGCT Hin1I 1193 GACGTC G[GA]^CG[CT]C 1701 GGCGTC NaeI 152 GCCGGC GCC^GGC 850 GCCGGC NcoI 1295 CCATGG C^CATGG 2730 CCATGG NgoMIV 152 GCCGGC G^CCGGC 850 GCCGGC SbfI 2221 CCTGCAGG CCTGCA^GG 2353 CCTGCAGG Sse8387I 2221 CCTGCAGG CCTGCA^GG 2353 CCTGCAGG XmnI 1160 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 4409 GAATGGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 138 enzymes) # ######################################### AccI AccII AciI AfaI AflIII AluI Alw44I AlwI AlwNI ApaLI AspHI AvaI AvaII BalI BanI BanII BbrPI BbsI BbvCI BbvI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrFI BsrGI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoO65I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SphI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmaCI XmaI