##################################
# NO CUT ENZYMES (   70 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AgeI        
AhdI        ApaI        AscI        AseI        AsnI        
Asp718I     AspEI       AviII       BbeI        BciVI       
BclI        BfrI        BsaBI       BseAI       BsiWI       
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BssHII      BssSI       BstBI       ClaI        CpoI        
DraI        EagI        Eam1105I    EclXI       Eco52I      
FbaI        FseI        FspI        HindIII     HpaI        
KasI        KpnI        MamI        MfeI        MluI        
MroI        MunI        NarI        NdeI        NheI        
NotI        NruI        NspV        PacI        PinAI       
PmeI        PshBI       Psp1406I    RcaI        RsrII       
SalI        ScaI        SexAI       SfoI        SfuI        
SgrAI       SnaBI       SspI        SwaI        XbaI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

AatII        1193  GACGTC               GACGT^C
AcsI         2778  GAATTC               [GA]^AATT[CT]
ApoI         2778  GAATTC               [GA]^AATT[CT]
AspI          471  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI        2824  GGATCC               G^GATCC
BcgI         3410  GCAAAAGGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaWI        4191  TCCGGT               [AT]^CCGG[AT]
BsiEI        4861  CGATCG               CG[GA][CT]^CG
BsmI         3963  GAATGC               GAATGC(1/-1)
BsrDI         463  GCAATG               GCAATG(2/0)
Bst1107I     3780  GTATAC               GTA^TAC
BstZ17I      3780  GTATAC               GTA^TAC
DrdI          960  GACTCGCTTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        2778  GAATTC               G^AATTC
EcoRV        3674  GATATC               GAT^ATC
EcoT22I       753  ATGCAT               ATGCA^T
HincII        124  GTTGAC               GT[CT]^[GA]AC
HindII        124  GTTGAC               GT[CT]^[GA]AC
KspI           38  CCGCGG               CCGC^GG
NsiI          753  ATGCAT               ATGCA^T
PaeR7I       1533  CTCGAG               C^TCGAG
PflFI         471  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        1662  GACCAGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI         4861  CGATCG               CGAT^CG
SacII          38  CCGCGG               CCGC^GG
SapI         2407  GCTCTTC              GCTCTTC(1/4)
SfiI          818  GGCCACACAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI         3688  ACTAGT               A^CTAGT
Tth111I       471  GACCATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI         1142  CCAGGCCCTCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI         1533  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AcyI         1193  GACGTC               G[GA]^CG[CT]C
             1701  GGCGTC              
AocI          707  CCTCAGG              CC^T[ACGT]AGG
             3843  CCTAAGG             
Aor51HI      1636  AGCGCT               AGC^GCT
             3745  AGCGCT              
Asp700I      1160  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4409  GAATGGATTC          
AvrII        1811  CCTAGG               C^CTAGG
             4053  CCTAGG              
BlnI         1811  CCTAGG               C^CTAGG
             4053  CCTAGG              
BsaHI        1193  GACGTC               G[GA]^CG[CT]C
             1701  GGCGTC              
Bsu36I        707  CCTCAGG              CC^T[ACGT]AGG
             3843  CCTAAGG             
Eco47III     1636  AGCGCT               AGC^GCT
             3745  AGCGCT              
Eco81I        707  CCTCAGG              CC^T[ACGT]AGG
             3843  CCTAAGG             
FauI          932  GCGGG                CCCGC(4/6)
             1433  GCGGG               
HaeII        1636  AGCGCT               [GA]GCGC^[CT]
             3745  AGCGCT              
Hin1I        1193  GACGTC               G[GA]^CG[CT]C
             1701  GGCGTC              
NaeI          152  GCCGGC               GCC^GGC
              850  GCCGGC              
NcoI         1295  CCATGG               C^CATGG
             2730  CCATGG              
NgoMIV        152  GCCGGC               G^CCGGC
              850  GCCGGC              
SbfI         2221  CCTGCAGG             CCTGCA^GG
             2353  CCTGCAGG            
Sse8387I     2221  CCTGCAGG             CCTGCA^GG
             2353  CCTGCAGG            
XmnI         1160  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             4409  GAATGGATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  138 enzymes) #
#########################################

AccI        AccII       AciI        AfaI        AflIII      
AluI        Alw44I      AlwI        AlwNI       ApaLI       
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbrPI       BbsI        BbvCI       BbvI        
BcnI        BfaI        BglI        BglII       BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1286I    Bsp1407I    BspMI       BsrFI       
BsrGI       BsrI        BssKI       BstAPI      BstEII      
BstF5I      BstNI       BstPI       BstUI       BstXI       
BstYI       Cac8I       CelII       CfoI        Cfr10I      
Cfr13I      DdeI        DpnII       DraII       DraIII      
DsaI        EaeI        EarI        EcoNI       EcoO109I    
EcoO65I     EcoRII      EcoT14I     Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PmaCI       PmlI        
PpuMI       PstI        PvuII       RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        SmlI        SphI        SspBI       StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       XmaCI       XmaI