##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI ApaI AscI AseI AsnI
Asp718I AspEI AviII BbeI BciVI
BclI BfrI BsaBI BseAI BsiWI
BspDI BspEI BspHI BspLU11I BsrBI
BssHII BssSI BstBI ClaI CpoI
DraI EagI Eam1105I EclXI Eco52I
FbaI FseI FspI HindIII HpaI
KasI KpnI MamI MfeI MluI
MroI MunI NarI NdeI NheI
NotI NruI NspV PacI PinAI
PmeI PshBI Psp1406I RcaI RsrII
SalI ScaI SexAI SfoI SfuI
SgrAI SnaBI SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AatII 1193 GACGTC GACGT^C
AcsI 2778 GAATTC [GA]^AATT[CT]
ApoI 2778 GAATTC [GA]^AATT[CT]
AspI 471 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2824 GGATCC G^GATCC
BcgI 3410 GCAAAAGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaWI 4191 TCCGGT [AT]^CCGG[AT]
BsiEI 4861 CGATCG CG[GA][CT]^CG
BsmI 3963 GAATGC GAATGC(1/-1)
BsrDI 463 GCAATG GCAATG(2/0)
Bst1107I 3780 GTATAC GTA^TAC
BstZ17I 3780 GTATAC GTA^TAC
DrdI 960 GACTCGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2778 GAATTC G^AATTC
EcoRV 3674 GATATC GAT^ATC
EcoT22I 753 ATGCAT ATGCA^T
HincII 124 GTTGAC GT[CT]^[GA]AC
HindII 124 GTTGAC GT[CT]^[GA]AC
KspI 38 CCGCGG CCGC^GG
NsiI 753 ATGCAT ATGCA^T
PaeR7I 1533 CTCGAG C^TCGAG
PflFI 471 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1662 GACCAGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 4861 CGATCG CGAT^CG
SacII 38 CCGCGG CCGC^GG
SapI 2407 GCTCTTC GCTCTTC(1/4)
SfiI 818 GGCCACACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 3688 ACTAGT A^CTAGT
Tth111I 471 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1142 CCAGGCCCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1533 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 1193 GACGTC G[GA]^CG[CT]C
1701 GGCGTC
AocI 707 CCTCAGG CC^T[ACGT]AGG
3843 CCTAAGG
Aor51HI 1636 AGCGCT AGC^GCT
3745 AGCGCT
Asp700I 1160 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4409 GAATGGATTC
AvrII 1811 CCTAGG C^CTAGG
4053 CCTAGG
BlnI 1811 CCTAGG C^CTAGG
4053 CCTAGG
BsaHI 1193 GACGTC G[GA]^CG[CT]C
1701 GGCGTC
Bsu36I 707 CCTCAGG CC^T[ACGT]AGG
3843 CCTAAGG
Eco47III 1636 AGCGCT AGC^GCT
3745 AGCGCT
Eco81I 707 CCTCAGG CC^T[ACGT]AGG
3843 CCTAAGG
FauI 932 GCGGG CCCGC(4/6)
1433 GCGGG
HaeII 1636 AGCGCT [GA]GCGC^[CT]
3745 AGCGCT
Hin1I 1193 GACGTC G[GA]^CG[CT]C
1701 GGCGTC
NaeI 152 GCCGGC GCC^GGC
850 GCCGGC
NcoI 1295 CCATGG C^CATGG
2730 CCATGG
NgoMIV 152 GCCGGC G^CCGGC
850 GCCGGC
SbfI 2221 CCTGCAGG CCTGCA^GG
2353 CCTGCAGG
Sse8387I 2221 CCTGCAGG CCTGCA^GG
2353 CCTGCAGG
XmnI 1160 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4409 GAATGGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 138 enzymes) #
#########################################
AccI AccII AciI AfaI AflIII
AluI Alw44I AlwI AlwNI ApaLI
AspHI AvaI AvaII BalI BanI
BanII BbrPI BbsI BbvCI BbvI
BcnI BfaI BglI BglII BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I Bsp1407I BspMI BsrFI
BsrGI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoO65I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SphI SspBI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmaCI XmaI