################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII BamHI BanI BbeI BglI BsaBI BsaHI BseAI BsiEI BspDI BspEI BssHII Bst1107I BstAPI BstEII BstPI BstZ17I ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I EcoO65I FseI FspI Hin1I HpaI KasI KpnI MamI MfeI MroI MunI NarI NdeI NotI NruI PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SpeI Sse8387I SwaI Tth111I ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 996 GTCTAC GT^[AC][GT]AC AflII 2662 CTTAAG C^TTAAG Aor51HI 624 AGCGCT AGC^GCT BbrPI 4578 CACGTG CAC^GTG BbvCI 2148 GCTGAGG CCTCAGC(-5/-2) BcgI 1159 GCAGAAGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 5617 GGATAC GTATCC(6/5) BclI 6101 TGATCA T^GATCA BfrI 2662 CTTAAG C^TTAAG BlpI 5194 GCTAAGC GC^T[ACGT]AGC Bpu1102I 5194 GCTAAGC GC^T[ACGT]AGC BsaWI 830 ACCGGA [AT]^CCGG[AT] BsiWI 4283 CGTACG C^GTACG BsmBI 5049 CGTCTC CGTCTC(1/5) Bsp1407I 6008 TGTACA T^GTACA BsrGI 6008 TGTACA T^GTACA CelII 5194 GCTAAGC GC^T[ACGT]AGC DraIII 5132 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 624 AGCGCT AGC^GCT EcoRV 4248 GATATC GAT^ATC EcoT22I 5675 ATGCAT ATGCA^T FbaI 6101 TGATCA T^GATCA KspI 7 CCGCGG CCGC^GG MluI 2535 ACGCGT A^CGCGT NcoI 3996 CCATGG C^CATGG NsiI 5675 ATGCAT ATGCA^T PaeR7I 4211 CTCGAG C^TCGAG PmaCI 4578 CACGTG CAC^GTG PmlI 4578 CACGTG CAC^GTG PshAI 1615 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 7 CCGCGG CCGC^GG SnaBI 4281 TACGTA TAC^GTA SspBI 6008 TGTACA T^GTACA SspI 6122 AATATT AAT^ATT XbaI 986 TCTAGA T^CTAGA XcmI 2096 CCAGACACGTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 4211 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AlwNI 1720 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG 5362 CAGCCACTG AocI 585 CCTCAGG CC^T[ACGT]AGG 3716 CCTCAGG Asp700I 1439 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3859 GAAGCGATTC AvrII 1835 CCTAGG C^CTAGG 5461 CCTAGG BalI 1826 TGGCCA TGG^CCA 5384 TGGCCA BglII 63 AGATCT A^GATCT 5441 AGATCT BlnI 1835 CCTAGG C^CTAGG 5461 CCTAGG BsaI 2413 GAGACC GGTCTC(1/5) 4035 GAGACC BspLU11I 2048 ACATGT A^CATGT 2277 ACATGT BspMI 5894 ACCTGC ACCTGC(4/8) 939 GCAGGT BsrBI 658 GAGCGG CCGCTC(-3/-3) 5323 GAGCGG BstBI 316 TTCGAA TT^CGAA 389 TTCGAA BstXI 1817 CCACAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 5365 CCACTGGAATGG Bsu36I 585 CCTCAGG CC^T[ACGT]AGG 3716 CCTCAGG Eco81I 585 CCTCAGG CC^T[ACGT]AGG 3716 CCTCAGG MluNI 1826 TGGCCA TGG^CCA 5384 TGGCCA MscI 1826 TGGCCA TGG^CCA 5384 TGGCCA NaeI 1470 GCCGGC GCC^GGC 5540 GCCGGC NgoMIV 1470 GCCGGC G^CCGGC 5540 GCCGGC NheI 2437 GCTAGC G^CTAGC 5025 GCTAGC NspV 316 TTCGAA TT^CGAA 389 TTCGAA PpuMI 2308 AGGACCC [GA]G^G[AT]CC[CT] 2781 AGGTCCC SapI 3202 GAAGAGC GCTCTTC(1/4) 3547 GAAGAGC SfuI 316 TTCGAA TT^CGAA 389 TTCGAA SmaI 2785 CCCGGG CCC^GGG 5232 CCCGGG StuI 1286 AGGCCT AGG^CCT 2697 AGGCCT XmaCI 2785 CCCGGG C^CCGGG 5232 CCCGGG XmaI 2785 CCCGGG C^CCGGG 5232 CCCGGG XmnI 1439 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3859 GAAGCGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI ApaI ApoI AspHI AvaI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspHI BsrDI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PstI PvuII RcaI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII