##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AviII BamHI BanI BbeI BglI
BsaBI BsaHI BseAI BsiEI BspDI
BspEI BssHII Bst1107I BstAPI BstEII
BstPI BstZ17I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco52I EcoO65I
FseI FspI Hin1I HpaI KasI
KpnI MamI MfeI MroI MunI
NarI NdeI NotI NruI PacI
PflFI PinAI PmeI PshBI Psp1406I
PvuI RsrII SalI SbfI ScaI
SexAI SfiI SfoI SgrAI SpeI
Sse8387I SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 996 GTCTAC GT^[AC][GT]AC
AflII 2662 CTTAAG C^TTAAG
Aor51HI 624 AGCGCT AGC^GCT
BbrPI 4578 CACGTG CAC^GTG
BbvCI 2148 GCTGAGG CCTCAGC(-5/-2)
BcgI 1159 GCAGAAGGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 5617 GGATAC GTATCC(6/5)
BclI 6101 TGATCA T^GATCA
BfrI 2662 CTTAAG C^TTAAG
BlpI 5194 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 5194 GCTAAGC GC^T[ACGT]AGC
BsaWI 830 ACCGGA [AT]^CCGG[AT]
BsiWI 4283 CGTACG C^GTACG
BsmBI 5049 CGTCTC CGTCTC(1/5)
Bsp1407I 6008 TGTACA T^GTACA
BsrGI 6008 TGTACA T^GTACA
CelII 5194 GCTAAGC GC^T[ACGT]AGC
DraIII 5132 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 624 AGCGCT AGC^GCT
EcoRV 4248 GATATC GAT^ATC
EcoT22I 5675 ATGCAT ATGCA^T
FbaI 6101 TGATCA T^GATCA
KspI 7 CCGCGG CCGC^GG
MluI 2535 ACGCGT A^CGCGT
NcoI 3996 CCATGG C^CATGG
NsiI 5675 ATGCAT ATGCA^T
PaeR7I 4211 CTCGAG C^TCGAG
PmaCI 4578 CACGTG CAC^GTG
PmlI 4578 CACGTG CAC^GTG
PshAI 1615 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 7 CCGCGG CCGC^GG
SnaBI 4281 TACGTA TAC^GTA
SspBI 6008 TGTACA T^GTACA
SspI 6122 AATATT AAT^ATT
XbaI 986 TCTAGA T^CTAGA
XcmI 2096 CCAGACACGTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 4211 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwNI 1720 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
5362 CAGCCACTG
AocI 585 CCTCAGG CC^T[ACGT]AGG
3716 CCTCAGG
Asp700I 1439 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3859 GAAGCGATTC
AvrII 1835 CCTAGG C^CTAGG
5461 CCTAGG
BalI 1826 TGGCCA TGG^CCA
5384 TGGCCA
BglII 63 AGATCT A^GATCT
5441 AGATCT
BlnI 1835 CCTAGG C^CTAGG
5461 CCTAGG
BsaI 2413 GAGACC GGTCTC(1/5)
4035 GAGACC
BspLU11I 2048 ACATGT A^CATGT
2277 ACATGT
BspMI 5894 ACCTGC ACCTGC(4/8)
939 GCAGGT
BsrBI 658 GAGCGG CCGCTC(-3/-3)
5323 GAGCGG
BstBI 316 TTCGAA TT^CGAA
389 TTCGAA
BstXI 1817 CCACAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
5365 CCACTGGAATGG
Bsu36I 585 CCTCAGG CC^T[ACGT]AGG
3716 CCTCAGG
Eco81I 585 CCTCAGG CC^T[ACGT]AGG
3716 CCTCAGG
MluNI 1826 TGGCCA TGG^CCA
5384 TGGCCA
MscI 1826 TGGCCA TGG^CCA
5384 TGGCCA
NaeI 1470 GCCGGC GCC^GGC
5540 GCCGGC
NgoMIV 1470 GCCGGC G^CCGGC
5540 GCCGGC
NheI 2437 GCTAGC G^CTAGC
5025 GCTAGC
NspV 316 TTCGAA TT^CGAA
389 TTCGAA
PpuMI 2308 AGGACCC [GA]G^G[AT]CC[CT]
2781 AGGTCCC
SapI 3202 GAAGAGC GCTCTTC(1/4)
3547 GAAGAGC
SfuI 316 TTCGAA TT^CGAA
389 TTCGAA
SmaI 2785 CCCGGG CCC^GGG
5232 CCCGGG
StuI 1286 AGGCCT AGG^CCT
2697 AGGCCT
XmaCI 2785 CCCGGG C^CCGGG
5232 CCCGGG
XmaI 2785 CCCGGG C^CCGGG
5232 CCCGGG
XmnI 1439 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3859 GAAGCGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI ApaI ApoI AspHI
AvaI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspHI BsrDI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRI EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RcaI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SphI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII