##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvrII BamHI BbrPI BbvCI
BglI BlnI BsaAI BsaBI BseAI
BsgI BsiEI BsiWI BspEI BssHII
BstBI BstXI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
FseI HpaI KpnI KspI MamI
MluI MroI NdeI NheI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SacII SapI SbfI
ScaI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I Tth111I
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AflIII 5372 ACATGT A^C[GA][CT]GT
AocI 1350 CCTGAGG CC^T[ACGT]AGG
AviII 2510 TGCGCA TGC^GCA
BbeI 1434 GGCGCC GGCGC^C
BciVI 2274 GTATCC GTATCC(6/5)
BclI 4401 TGATCA T^GATCA
BglII 4884 AGATCT A^GATCT
BsaWI 2669 TCCGGT [AT]^CCGG[AT]
Bsp1407I 1887 TGTACA T^GTACA
BspDI 1455 ATCGAT AT^CGAT
BspHI 614 TCATGA T^CATGA
BspLU11I 5372 ACATGT A^CATGT
BsrBI 121 GAGCGG CCGCTC(-3/-3)
BsrFI 1506 GCCGGC [GA]^CCGG[CT]
BsrGI 1887 TGTACA T^GTACA
BstEII 3111 GGTGACC G^GT[ACGT]ACC
BstPI 3111 GGTGACC G^GT[ACGT]ACC
Bsu36I 1350 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1506 GCCGGC [GA]^CCGG[CT]
ClaI 1455 ATCGAT AT^CGAT
DraIII 2204 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1350 CCTGAGG CC^T[ACGT]AGG
EcoO65I 3111 GGTGACC G^GT[ACGT]ACC
EcoRV 1838 GATATC GAT^ATC
FbaI 4401 TGATCA T^GATCA
FspI 2510 TGCGCA TGC^GCA
HaeII 1434 GGCGCC [GA]GCGC^[CT]
KasI 1434 GGCGCC G^GCGCC
MfeI 1111 CAATTG C^AATTG
MslI 207 CATTACGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1111 CAATTG C^AATTG
NaeI 1506 GCCGGC GCC^GGC
NarI 1434 GGCGCC GG^CGCC
NgoMIV 1506 GCCGGC G^CCGGC
PaeR7I 565 CTCGAG C^TCGAG
PflMI 1320 CCATATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 1768 CTGCAG CTGCA^G
RcaI 614 TCATGA T^CATGA
SacI 1338 GAGCTC GAGCT^C
SalI 1734 GTCGAC G^TCGAC
SexAI 382 ACCAGGT A^CC[AT]GGT
SfoI 1434 GGCGCC GGC^GCC
SmaI 2976 CCCGGG CCC^GGG
SspBI 1887 TGTACA T^GTACA
StuI 3568 AGGCCT AGG^CCT
SwaI 4638 ATTTAAAT ATTT^AAAT
Van91I 1320 CCATATAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2775 TCTAGA T^CTAGA
XhoI 565 CTCGAG C^TCGAG
XmaCI 2976 CCCGGG C^CCGGG
XmaI 2976 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccII 1593 CGCG CG^CG
2162 CGCG
Asp700I 625 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4122 GAATACGTTC
BanI 1434 GGCGCC G^G[CT][GA]CC
4010 GGCACC
BcgI 567 CGAGTGCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1447 GCATTGTGATCG
BsmBI 1789 CGTCTC CGTCTC(1/5)
2908 CGTCTC
BsmI 1427 GAATGC GAATGC(1/-1)
5026 GCATTC
Bst1107I 3670 GTATAC GTA^TAC
5255 GTATAC
BstAPI 303 GCATTGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1181 GCAGCAGCTGC
BstUI 1593 CGCG CG^CG
2162 CGCG
BstZ17I 3670 GTATAC GTA^TAC
5255 GTATAC
DraII 2795 AGGTCCT [GA]G^G[ACGT]CC[CT]
3445 AGGTCCC
EarI 1806 GAAGAG CTCTTC(1/4)
2085 GAAGAG
EcoO109I 2795 AGGTCCT [GA]G^G[ACGT]CC[CT]
3445 AGGTCCC
EcoRI 3539 GAATTC G^AATTC
4874 GAATTC
EcoT22I 301 ATGCAT ATGCA^T
2485 ATGCAT
FauI 123 GCGGG CCCGC(4/6)
577 GCGGG
HindIII 2379 AAGCTT A^AGCTT
4731 AAGCTT
Ksp632I 1806 GAAGAG CTCTTC(1/4)
2085 GAAGAG
MvnI 1593 CGCG CG^CG
2162 CGCG
NsiI 301 ATGCAT ATGCA^T
2485 ATGCAT
PpuMI 2795 AGGTCCT [GA]G^G[AT]CC[CT]
3445 AGGTCCC
PshAI 2967 GACTGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2984 GACTACTGTC
XcmI 988 CCAGAGATTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1217 CCACCCGTTTTTTGG
XmnI 625 GAATGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4122 GAATACGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AflII AluI AlwI AlwNI ApoI
AspHI AvaI AvaII BalI BanII
BbsI BbvI BcnI BfaI BfrI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BssSI BstF5I BstNI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DsaI EaeI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII