##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
Acc65I AclI AgeI Aor51HI ApaI
AscI AseI AsnI Asp718I AvrII
BbvCI BlnI BsiWI BspDI BspHI
BspMI BssSI ClaI DraI EagI
EclXI Eco47III Eco52I EcoRV FseI
HindIII HpaI KpnI MfeI MslI
MunI NheI NotI PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
RcaI SapI SbfI SexAI SfiI
SgrAI SnaBI SpeI Sse8387I StuI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 2103 GACGTC GACGT^C
AccIII 2078 TCCGGA T^CCGGA
AflII 4551 CTTAAG C^TTAAG
Asp700I 3108 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 4647 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3635 TGCGCA TGC^GCA
BamHI 2761 GGATCC G^GATCC
BcgI 2108 CGACGTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4551 CTTAAG C^TTAAG
BsaI 4265 GAGACC GGTCTC(1/5)
BseAI 2078 TCCGGA T^CCGGA
BsmI 1760 GAATGC GAATGC(1/-1)
BspEI 2078 TCCGGA T^CCGGA
BspLU11I 2996 ACATGT A^CATGT
BsrDI 241 GCAATG GCAATG(2/0)
BstAPI 1160 GCAGGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1029 TTCGAA TT^CGAA
BstEII 1664 GGTGACC G^GT[ACGT]ACC
BstPI 1664 GGTGACC G^GT[ACGT]ACC
CpoI 273 CGGACCG CG^G[AT]CCG
DraIII 4728 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 1664 GGTGACC G^GT[ACGT]ACC
FspI 3635 TGCGCA TGC^GCA
KspI 2164 CCGCGG CCGC^GG
MluI 3810 ACGCGT A^CGCGT
MroI 2078 TCCGGA T^CCGGA
NaeI 53 GCCGGC GCC^GGC
NdeI 3157 CATATG CA^TATG
NgoMIV 53 GCCGGC G^CCGGC
NruI 31 TCGCGA TCG^CGA
NspV 1029 TTCGAA TT^CGAA
PflFI 4647 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2604 GGGACCT [GA]G^G[AT]CC[CT]
PvuI 2062 CGATCG CGAT^CG
PvuII 260 CAGCTG CAG^CTG
RsrII 273 CGGACCG CG^G[AT]CCG
SacII 2164 CCGCGG CCGC^GG
SalI 2106 GTCGAC G^TCGAC
SfuI 1029 TTCGAA TT^CGAA
SmaI 2264 CCCGGG CCC^GGG
SphI 4707 GCATGC GCATG^C
SspI 4619 AATATT AAT^ATT
Tth111I 4647 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2264 CCCGGG C^CCGGG
XmaI 2264 CCCGGG C^CCGGG
XmnI 3108 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AhdI 1889 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4837 GACTGTCCGTC
Alw44I 2187 GTGCAC G^TGCAC
2382 GTGCAC
AlwNI 1161 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
1297 CAGTTTCTG
AocI 1049 CCTGAGG CC^T[ACGT]AGG
2797 CCTCAGG
ApaLI 2187 GTGCAC G^TGCAC
2382 GTGCAC
AspEI 1889 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4837 GACTGTCCGTC
AvaI 861 CCCGAG C^[CT]CG[GA]G
2264 CCCGGG
BbeI 94 GGCGCC GGCGC^C
1135 GGCGCC
BciVI 412 GGATAC GTATCC(6/5)
921 GGATAC
BclI 1705 TGATCA T^GATCA
4361 TGATCA
BglII 839 AGATCT A^GATCT
1009 AGATCT
BlpI 1490 GCTGAGC GC^T[ACGT]AGC
2545 GCTGAGC
Bpu1102I 1490 GCTGAGC GC^T[ACGT]AGC
2545 GCTGAGC
BsaBI 456 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
564 GATCTGCATC
BsgI 293 CTGCAC GTGCAG(16/14)
465 CTGCAC
BsiEI 1991 CGGTCG CG[GA][CT]^CG
2062 CGATCG
BsmBI 2661 CGTCTC CGTCTC(1/5)
3849 CGTCTC
BsoBI 861 CCCGAG C^[CT]CG[GA]G
2264 CCCGGG
Bsp1407I 1639 TGTACA T^GTACA
4818 TGTACA
BsrGI 1639 TGTACA T^GTACA
4818 TGTACA
Bst1107I 1250 GTATAC GTA^TAC
3002 GTATAC
BstZ17I 1250 GTATAC GTA^TAC
3002 GTATAC
Bsu36I 1049 CCTGAGG CC^T[ACGT]AGG
2797 CCTCAGG
CelII 1490 GCTGAGC GC^T[ACGT]AGC
2545 GCTGAGC
Eam1105I 1889 GACCAAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4837 GACTGTCCGTC
Eco81I 1049 CCTGAGG CC^T[ACGT]AGG
2797 CCTCAGG
EcoNI 74 CCTGCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1139 CCTCACCCAGG
EcoRI 547 GAATTC G^AATTC
1116 GAATTC
EcoT22I 3180 ATGCAT ATGCA^T
4705 ATGCAT
FbaI 1705 TGATCA T^GATCA
4361 TGATCA
KasI 94 GGCGCC G^GCGCC
1135 GGCGCC
MamI 456 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
564 GATCTGCATC
NarI 94 GGCGCC GG^CGCC
1135 GGCGCC
NsiI 3180 ATGCAT ATGCA^T
4705 ATGCAT
SacI 14 GAGCTC GAGCT^C
2696 GAGCTC
SfoI 94 GGCGCC GGC^GCC
1135 GGCGCC
SspBI 1639 TGTACA T^GTACA
4818 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI ApoI
AspHI AvaII BalI BanI BanII
BbrPI BbsI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaAI
BsaHI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrBI BsrFI BsrI BssHII BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PstI RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII