################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## AclI AflII AhdI Aor51HI AscI AspEI AspI AviII BamHI BclI BfrI BsaBI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII Bst1107I BstZ17I ClaI CpoI DrdI Eam1105I Eco47III FbaI FseI FspI MamI MfeI MluI MunI NdeI NotI NruI PacI PaeR7I PflFI PmeI Psp1406I PvuI RcaI RsrII SalI ScaI SexAI SfiI SgrAI SnaBI SpeI SspBI SspI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AatII 2907 GACGTC GACGT^C Acc65I 4913 GGTACC G^GTACC AflIII 3652 ACACGT A^C[GA][CT]GT AgeI 2682 ACCGGT A^CCGGT ApaI 1606 GGGCCC GGGCC^C AseI 1646 ATTAAT AT^TAAT AsnI 1646 ATTAAT AT^TAAT Asp718I 4913 GGTACC G^GTACC AvrII 4796 CCTAGG C^CTAGG BbeI 3731 GGCGCC GGCGC^C BlnI 4796 CCTAGG C^CTAGG BsmI 3391 GAATGC GAATGC(1/-1) BssSI 3474 CACGAG CACGAG(-5/-1) EagI 2385 CGGCCG C^GGCCG EclXI 2385 CGGCCG C^GGCCG Eco52I 2385 CGGCCG C^GGCCG EcoRV 1170 GATATC GAT^ATC EcoT22I 550 ATGCAT ATGCA^T HpaI 77 GTTAAC GTT^AAC KasI 3731 GGCGCC G^GCGCC KpnI 4913 GGTACC GGTAC^C KspI 24 CCGCGG CCGC^GG NaeI 3975 GCCGGC GCC^GGC NarI 3731 GGCGCC GG^CGCC NgoMIV 3975 GCCGGC G^CCGGC NheI 4451 GCTAGC G^CTAGC NsiI 550 ATGCAT ATGCA^T PinAI 2682 ACCGGT A^CCGGT PshAI 3596 GACGGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 1646 ATTAAT AT^TAAT SacII 24 CCGCGG CCGC^GG SbfI 3446 CCTGCAGG CCTGCA^GG SfoI 3731 GGCGCC GGC^GCC SmaI 5017 CCCGGG CCC^GGG Sse8387I 3446 CCTGCAGG CCTGCA^GG XbaI 2876 TCTAGA T^CTAGA XmaCI 5017 CCCGGG C^CCGGG XmaI 5017 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccI 4384 GTAGAC GT^[AC][GT]AC 4986 GTCTAC AccII 25 CGCG CG^CG 3053 CGCG AccIII 410 TCCGGA T^CCGGA 1070 TCCGGA BbrPI 1766 CACGTG CAC^GTG 3653 CACGTG BbvCI 1734 GCTGAGG CCTCAGC(-5/-2) 4351 GCTGAGG BseAI 410 TCCGGA T^CCGGA 1070 TCCGGA BsiEI 2385 CGGCCG CG[GA][CT]^CG 2684 CGGTCG BspEI 410 TCCGGA T^CCGGA 1070 TCCGGA BsrDI 1520 CATTGC GCAATG(2/0) 1730 CATTGC BsrFI 2682 ACCGGT [GA]^CCGG[CT] 3975 GCCGGC BstAPI 2249 GCAGCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3551 GCAACCTGTGC BstEII 216 GGTCACC G^GT[ACGT]ACC 3089 GGTTACC BstPI 216 GGTCACC G^GT[ACGT]ACC 3089 GGTTACC BstUI 25 CGCG CG^CG 3053 CGCG Cfr10I 2682 ACCGGT [GA]^CCGG[CT] 3975 GCCGGC DraI 4758 TTTAAA TTT^AAA 5224 TTTAAA EcoNI 680 CCTGAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 719 CCTTTTCAAGG EcoO65I 216 GGTCACC G^GT[ACGT]ACC 3089 GGTTACC HincII 77 GTTAAC GT[CT]^[GA]AC 2997 GTTGAC HindII 77 GTTAAC GT[CT]^[GA]AC 2997 GTTGAC HindIII 190 AAGCTT A^AGCTT 563 AAGCTT MroI 410 TCCGGA T^CCGGA 1070 TCCGGA MvnI 25 CGCG CG^CG 3053 CGCG NcoI 1696 CCATGG C^CATGG 2758 CCATGG PflMI 1430 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3320 CCATTTGATGG PmaCI 1766 CACGTG CAC^GTG 3653 CACGTG PmlI 1766 CACGTG CAC^GTG 3653 CACGTG PvuII 1575 CAGCTG CAG^CTG 3308 CAGCTG SacI 1852 GAGCTC GAGCT^C 3699 GAGCTC Van91I 1430 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3320 CCATTTGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 134 enzymes) # ######################################### AciI AcsI AcyI AfaI AluI Alw44I AlwI AlwNI AocI ApaLI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcgI BciVI BcnI BfaI BglI BglII BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstBI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI Eco81I EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI NspV PleI PpuMI PstI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfuI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI