##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AclI AflII AhdI Aor51HI AscI
AspEI AspI AviII BamHI BclI
BfrI BsaBI BsiWI Bsp1407I BspDI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstZ17I ClaI CpoI DrdI Eam1105I
Eco47III FbaI FseI FspI MamI
MfeI MluI MunI NdeI NotI
NruI PacI PaeR7I PflFI PmeI
Psp1406I PvuI RcaI RsrII SalI
ScaI SexAI SfiI SgrAI SnaBI
SpeI SspBI SspI SwaI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 2907 GACGTC GACGT^C
Acc65I 4913 GGTACC G^GTACC
AflIII 3652 ACACGT A^C[GA][CT]GT
AgeI 2682 ACCGGT A^CCGGT
ApaI 1606 GGGCCC GGGCC^C
AseI 1646 ATTAAT AT^TAAT
AsnI 1646 ATTAAT AT^TAAT
Asp718I 4913 GGTACC G^GTACC
AvrII 4796 CCTAGG C^CTAGG
BbeI 3731 GGCGCC GGCGC^C
BlnI 4796 CCTAGG C^CTAGG
BsmI 3391 GAATGC GAATGC(1/-1)
BssSI 3474 CACGAG CACGAG(-5/-1)
EagI 2385 CGGCCG C^GGCCG
EclXI 2385 CGGCCG C^GGCCG
Eco52I 2385 CGGCCG C^GGCCG
EcoRV 1170 GATATC GAT^ATC
EcoT22I 550 ATGCAT ATGCA^T
HpaI 77 GTTAAC GTT^AAC
KasI 3731 GGCGCC G^GCGCC
KpnI 4913 GGTACC GGTAC^C
KspI 24 CCGCGG CCGC^GG
NaeI 3975 GCCGGC GCC^GGC
NarI 3731 GGCGCC GG^CGCC
NgoMIV 3975 GCCGGC G^CCGGC
NheI 4451 GCTAGC G^CTAGC
NsiI 550 ATGCAT ATGCA^T
PinAI 2682 ACCGGT A^CCGGT
PshAI 3596 GACGGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1646 ATTAAT AT^TAAT
SacII 24 CCGCGG CCGC^GG
SbfI 3446 CCTGCAGG CCTGCA^GG
SfoI 3731 GGCGCC GGC^GCC
SmaI 5017 CCCGGG CCC^GGG
Sse8387I 3446 CCTGCAGG CCTGCA^GG
XbaI 2876 TCTAGA T^CTAGA
XmaCI 5017 CCCGGG C^CCGGG
XmaI 5017 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 4384 GTAGAC GT^[AC][GT]AC
4986 GTCTAC
AccII 25 CGCG CG^CG
3053 CGCG
AccIII 410 TCCGGA T^CCGGA
1070 TCCGGA
BbrPI 1766 CACGTG CAC^GTG
3653 CACGTG
BbvCI 1734 GCTGAGG CCTCAGC(-5/-2)
4351 GCTGAGG
BseAI 410 TCCGGA T^CCGGA
1070 TCCGGA
BsiEI 2385 CGGCCG CG[GA][CT]^CG
2684 CGGTCG
BspEI 410 TCCGGA T^CCGGA
1070 TCCGGA
BsrDI 1520 CATTGC GCAATG(2/0)
1730 CATTGC
BsrFI 2682 ACCGGT [GA]^CCGG[CT]
3975 GCCGGC
BstAPI 2249 GCAGCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3551 GCAACCTGTGC
BstEII 216 GGTCACC G^GT[ACGT]ACC
3089 GGTTACC
BstPI 216 GGTCACC G^GT[ACGT]ACC
3089 GGTTACC
BstUI 25 CGCG CG^CG
3053 CGCG
Cfr10I 2682 ACCGGT [GA]^CCGG[CT]
3975 GCCGGC
DraI 4758 TTTAAA TTT^AAA
5224 TTTAAA
EcoNI 680 CCTGAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
719 CCTTTTCAAGG
EcoO65I 216 GGTCACC G^GT[ACGT]ACC
3089 GGTTACC
HincII 77 GTTAAC GT[CT]^[GA]AC
2997 GTTGAC
HindII 77 GTTAAC GT[CT]^[GA]AC
2997 GTTGAC
HindIII 190 AAGCTT A^AGCTT
563 AAGCTT
MroI 410 TCCGGA T^CCGGA
1070 TCCGGA
MvnI 25 CGCG CG^CG
3053 CGCG
NcoI 1696 CCATGG C^CATGG
2758 CCATGG
PflMI 1430 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3320 CCATTTGATGG
PmaCI 1766 CACGTG CAC^GTG
3653 CACGTG
PmlI 1766 CACGTG CAC^GTG
3653 CACGTG
PvuII 1575 CAGCTG CAG^CTG
3308 CAGCTG
SacI 1852 GAGCTC GAGCT^C
3699 GAGCTC
Van91I 1430 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3320 CCATTTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaLI
ApoI Asp700I AspHI AvaI AvaII
BalI BanI BanII BbsI BbvI
BcgI BciVI BcnI BfaI BglI
BglII BlpI BmyI BpmI Bpu1102I
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrI BssKI
BstBI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco81I EcoO109I EcoRI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI NspV PleI PpuMI
PstI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfuI SmlI
SphI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI