################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AatII Acc65I AccIII AgeI AhdI AscI Asp718I AspEI AviII AvrII BbeI BbrPI BbvCI BcgI BglI BlnI BseAI BsiWI BspDI BspEI BspHI BssHII Bst1107I BstBI BstZ17I ClaI CpoI Eam1105I EcoRV FseI FspI HindIII HpaI KasI KpnI KspI MfeI MluI MroI MunI NarI NotI NruI NspV PaeR7I PinAI PmaCI PmeI PmlI PshAI PstI PvuI PvuII RcaI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 4943 GTAGAC GT^[AC][GT]AC AclI 2085 AACGTT AA^CGTT AcyI 4321 GGCGTC G[GA]^CG[CT]C AflII 3555 CTTAAG C^TTAAG Alw44I 3608 GTGCAC G^TGCAC Aor51HI 5039 AGCGCT AGC^GCT ApaI 728 GGGCCC GGGCC^C ApaLI 3608 GTGCAC G^TGCAC AvaI 451 CCCGAG C^[CT]CG[GA]G BalI 2402 TGGCCA TGG^CCA BanI 3888 GGCACC G^G[CT][GA]CC BfrI 3555 CTTAAG C^TTAAG BsaAI 4457 TACGTG [CT]AC^GT[GA] BsaBI 1377 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 4321 GGCGTC G[GA]^CG[CT]C BsiEI 165 CGGCCG CG[GA][CT]^CG BsmI 3633 GCATTC GAATGC(1/-1) BsoBI 451 CCCGAG C^[CT]CG[GA]G BspLU11I 2318 ACATGT A^CATGT BsrFI 270 GCCGGC [GA]^CCGG[CT] BssSI 1690 CACGAG CACGAG(-5/-1) Cfr10I 270 GCCGGC [GA]^CCGG[CT] DraIII 5812 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 165 CGGCCG C^GGCCG EclXI 165 CGGCCG C^GGCCG Eco47III 5039 AGCGCT AGC^GCT Eco52I 165 CGGCCG C^GGCCG Hin1I 4321 GGCGTC G[GA]^CG[CT]C HincII 4392 GTCAAC GT[CT]^[GA]AC HindII 4392 GTCAAC GT[CT]^[GA]AC MamI 1377 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2402 TGGCCA TGG^CCA MscI 2402 TGGCCA TGG^CCA NaeI 270 GCCGGC GCC^GGC NdeI 2636 CATATG CA^TATG NgoMIV 270 GCCGGC G^CCGGC NheI 2944 GCTAGC G^CTAGC Psp1406I 2085 AACGTT AA^CGTT SapI 5138 GCTCTTC GCTCTTC(1/4) ScaI 1343 AGTACT AGT^ACT SphI 89 GCATGC GCATG^C StuI 5436 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccII 2563 CGCG CG^CG 5190 CGCG AflIII 2318 ACATGT A^C[GA][CT]GT 4458 ACGTGT AocI 1889 CCTTAGG CC^T[ACGT]AGG 2835 CCTGAGG AseI 925 ATTAAT AT^TAAT 2440 ATTAAT AsnI 925 ATTAAT AT^TAAT 2440 ATTAAT AspI 3244 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4868 GACAGAGTC BclI 465 TGATCA T^GATCA 3069 TGATCA BlpI 1008 GCTGAGC GC^T[ACGT]AGC 3163 GCTCAGC Bpu1102I 1008 GCTGAGC GC^T[ACGT]AGC 3163 GCTCAGC BsaWI 48 ACCGGA [AT]^CCGG[AT] 3308 ACCGGA BstEII 13 GGTAACC G^GT[ACGT]ACC 3424 GGTGACC BstPI 13 GGTAACC G^GT[ACGT]ACC 3424 GGTGACC BstUI 2563 CGCG CG^CG 5190 CGCG BstXI 1415 CCACACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3459 CCAAAACAATGG Bsu36I 1889 CCTTAGG CC^T[ACGT]AGG 2835 CCTGAGG CelII 1008 GCTGAGC GC^T[ACGT]AGC 3163 GCTCAGC DrdI 657 GACCTCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3241 GACGACCTGGTC Eco81I 1889 CCTTAGG CC^T[ACGT]AGG 2835 CCTGAGG EcoO65I 13 GGTAACC G^GT[ACGT]ACC 3424 GGTGACC EcoRI 135 GAATTC G^AATTC 1079 GAATTC EcoT22I 2633 ATGCAT ATGCA^T 3631 ATGCAT FbaI 465 TGATCA T^GATCA 3069 TGATCA HgaI 4488 GACGC GACGC(5/10) 4322 GCGTC MspA1I 94 CAGCGG C[AC]G^C[GT]G 3178 CAGCGG MvnI 2563 CGCG CG^CG 5190 CGCG NsiI 2633 ATGCAT ATGCA^T 3631 ATGCAT PacI 922 TTAATTAA TTAAT^TAA 5654 TTAATTAA PflFI 3244 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4868 GACAGAGTC PshBI 925 ATTAAT AT^TAAT 2440 ATTAAT SexAI 3245 ACCTGGT A^CC[AT]GGT 3326 ACCAGGT Tth111I 3244 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4868 GACAGAGTC XbaI 2061 TCTAGA T^CTAGA 3395 TCTAGA XcmI 281 CCATGCCCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5337 CCAGCTCAGTTTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI AvaII BamHI BanII BbsI BbvI BciVI BcnI BfaI BglII BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I Bsp1407I BspMI BsrBI BsrDI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI