##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AccIII AgeI AhdI
AscI Asp718I AspEI AviII AvrII
BbeI BbrPI BbvCI BcgI BglI
BlnI BseAI BsiWI BspDI BspEI
BspHI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI Eam1105I EcoRV FseI
FspI HindIII HpaI KasI KpnI
KspI MfeI MluI MroI MunI
NarI NotI NruI NspV PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PstI PvuI PvuII RcaI RsrII
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SwaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 4943 GTAGAC GT^[AC][GT]AC
AclI 2085 AACGTT AA^CGTT
AcyI 4321 GGCGTC G[GA]^CG[CT]C
AflII 3555 CTTAAG C^TTAAG
Alw44I 3608 GTGCAC G^TGCAC
Aor51HI 5039 AGCGCT AGC^GCT
ApaI 728 GGGCCC GGGCC^C
ApaLI 3608 GTGCAC G^TGCAC
AvaI 451 CCCGAG C^[CT]CG[GA]G
BalI 2402 TGGCCA TGG^CCA
BanI 3888 GGCACC G^G[CT][GA]CC
BfrI 3555 CTTAAG C^TTAAG
BsaAI 4457 TACGTG [CT]AC^GT[GA]
BsaBI 1377 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 4321 GGCGTC G[GA]^CG[CT]C
BsiEI 165 CGGCCG CG[GA][CT]^CG
BsmI 3633 GCATTC GAATGC(1/-1)
BsoBI 451 CCCGAG C^[CT]CG[GA]G
BspLU11I 2318 ACATGT A^CATGT
BsrFI 270 GCCGGC [GA]^CCGG[CT]
BssSI 1690 CACGAG CACGAG(-5/-1)
Cfr10I 270 GCCGGC [GA]^CCGG[CT]
DraIII 5812 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 165 CGGCCG C^GGCCG
EclXI 165 CGGCCG C^GGCCG
Eco47III 5039 AGCGCT AGC^GCT
Eco52I 165 CGGCCG C^GGCCG
Hin1I 4321 GGCGTC G[GA]^CG[CT]C
HincII 4392 GTCAAC GT[CT]^[GA]AC
HindII 4392 GTCAAC GT[CT]^[GA]AC
MamI 1377 GATAGTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2402 TGGCCA TGG^CCA
MscI 2402 TGGCCA TGG^CCA
NaeI 270 GCCGGC GCC^GGC
NdeI 2636 CATATG CA^TATG
NgoMIV 270 GCCGGC G^CCGGC
NheI 2944 GCTAGC G^CTAGC
Psp1406I 2085 AACGTT AA^CGTT
SapI 5138 GCTCTTC GCTCTTC(1/4)
ScaI 1343 AGTACT AGT^ACT
SphI 89 GCATGC GCATG^C
StuI 5436 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccII 2563 CGCG CG^CG
5190 CGCG
AflIII 2318 ACATGT A^C[GA][CT]GT
4458 ACGTGT
AocI 1889 CCTTAGG CC^T[ACGT]AGG
2835 CCTGAGG
AseI 925 ATTAAT AT^TAAT
2440 ATTAAT
AsnI 925 ATTAAT AT^TAAT
2440 ATTAAT
AspI 3244 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4868 GACAGAGTC
BclI 465 TGATCA T^GATCA
3069 TGATCA
BlpI 1008 GCTGAGC GC^T[ACGT]AGC
3163 GCTCAGC
Bpu1102I 1008 GCTGAGC GC^T[ACGT]AGC
3163 GCTCAGC
BsaWI 48 ACCGGA [AT]^CCGG[AT]
3308 ACCGGA
BstEII 13 GGTAACC G^GT[ACGT]ACC
3424 GGTGACC
BstPI 13 GGTAACC G^GT[ACGT]ACC
3424 GGTGACC
BstUI 2563 CGCG CG^CG
5190 CGCG
BstXI 1415 CCACACCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3459 CCAAAACAATGG
Bsu36I 1889 CCTTAGG CC^T[ACGT]AGG
2835 CCTGAGG
CelII 1008 GCTGAGC GC^T[ACGT]AGC
3163 GCTCAGC
DrdI 657 GACCTCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3241 GACGACCTGGTC
Eco81I 1889 CCTTAGG CC^T[ACGT]AGG
2835 CCTGAGG
EcoO65I 13 GGTAACC G^GT[ACGT]ACC
3424 GGTGACC
EcoRI 135 GAATTC G^AATTC
1079 GAATTC
EcoT22I 2633 ATGCAT ATGCA^T
3631 ATGCAT
FbaI 465 TGATCA T^GATCA
3069 TGATCA
HgaI 4488 GACGC GACGC(5/10)
4322 GCGTC
MspA1I 94 CAGCGG C[AC]G^C[GT]G
3178 CAGCGG
MvnI 2563 CGCG CG^CG
5190 CGCG
NsiI 2633 ATGCAT ATGCA^T
3631 ATGCAT
PacI 922 TTAATTAA TTAAT^TAA
5654 TTAATTAA
PflFI 3244 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4868 GACAGAGTC
PshBI 925 ATTAAT AT^TAAT
2440 ATTAAT
SexAI 3245 ACCTGGT A^CC[AT]GGT
3326 ACCAGGT
Tth111I 3244 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4868 GACAGAGTC
XbaI 2061 TCTAGA T^CTAGA
3395 TCTAGA
XcmI 281 CCATGCCCCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5337 CCAGCTCAGTTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BamHI BanII BbsI BbvI BciVI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I Bsp1407I BspMI BsrBI
BsrDI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI