################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII Acc65I AclI AgeI Aor51HI ApaI AscI Asp718I AspI AviII AvrII BalI BbrPI BbvCI BlnI BsaBI BsaI BsiEI BsiWI BsmBI BspDI BsrFI BstBI BstEII BstPI BstXI Cfr10I ClaI CpoI DrdI EaeI EagI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV FseI FspI HpaI KpnI MamI MluI MluNI MscI NaeI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SalI SbfI SexAI SfiI SfuI SgrAI SnaBI Sse8387I SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 1204 GTATAC GT^[AC][GT]AC AccIII 5082 TCCGGA T^CCGGA AflII 440 CTTAAG C^TTAAG AhdI 3044 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1493 GTGCAC G^TGCAC ApaLI 1493 GTGCAC G^TGCAC AspEI 3044 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanI 151 GGCGCC G^G[CT][GA]CC BbeI 151 GGCGCC GGCGC^C BciVI 2308 GTATCC GTATCC(6/5) BclI 3250 TGATCA T^GATCA BfrI 440 CTTAAG C^TTAAG BglII 843 AGATCT A^GATCT BsaAI 3836 CACGTA [CT]AC^GT[GA] BseAI 5082 TCCGGA T^CCGGA BspEI 5082 TCCGGA T^CCGGA BssSI 5646 CTCGTG CACGAG(-5/-1) Bst1107I 1204 GTATAC GTA^TAC BstAPI 4448 GCAGAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 1204 GTATAC GTA^TAC Eam1105I 3044 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 3250 TGATCA T^GATCA HindIII 4736 AAGCTT A^AGCTT KasI 151 GGCGCC G^GCGCC MfeI 873 CAATTG C^AATTG MroI 5082 TCCGGA T^CCGGA MunI 873 CAATTG C^AATTG NarI 151 GGCGCC GG^CGCC NdeI 4702 CATATG CA^TATG NheI 1945 GCTAGC G^CTAGC SacI 2137 GAGCTC GAGCT^C SapI 2816 GAAGAGC GCTCTTC(1/4) SfoI 151 GGCGCC GGC^GCC SmaI 158 CCCGGG CCC^GGG SpeI 3164 ACTAGT A^CTAGT SphI 764 GCATGC GCATG^C XbaI 4523 TCTAGA T^CTAGA XcmI 867 CCAGAACAATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 158 CCCGGG C^CCGGG XmaI 158 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AcyI 151 GGCGCC G[GA]^CG[CT]C 4519 GGCGTC AocI 5228 CCTCAGG CC^T[ACGT]AGG 5814 CCTGAGG AspHI 1493 GTGCAC G[AT]GC[AT]^C 2137 GAGCTC BamHI 2941 GGATCC G^GATCC 3364 GGATCC BcgI 24 GCAGAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 5118 GCAGAAGCCTCG BglI 102 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 192 GCCGGGCGGGC BlpI 247 GCTAAGC GC^T[ACGT]AGC 1442 GCTCAGC Bpu1102I 247 GCTAAGC GC^T[ACGT]AGC 1442 GCTCAGC BsaHI 151 GGCGCC G[GA]^CG[CT]C 4519 GGCGTC BsiHKAI 1493 GTGCAC G[AT]GC[AT]^C 2137 GAGCTC BspHI 2779 TCATGA T^CATGA 3346 TCATGA BspLU11I 1429 ACATGT A^CATGT 1952 ACATGT BsrBI 323 CCGCTC CCGCTC(-3/-3) 811 GAGCGG BssHII 8 GCGCGC G^CGCGC 188 GCGCGC Bsu36I 5228 CCTCAGG CC^T[ACGT]AGG 5814 CCTGAGG CelII 247 GCTAAGC GC^T[ACGT]AGC 1442 GCTCAGC DraII 161 GGGTCCT [GA]G^G[ACGT]CC[CT] 2784 AGGTCCC DraIII 4530 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG 4532 CACAGTGTG Eco81I 5228 CCTCAGG CC^T[ACGT]AGG 5814 CCTGAGG EcoO109I 161 GGGTCCT [GA]G^G[ACGT]CC[CT] 2784 AGGTCCC EcoRI 781 GAATTC G^AATTC 5668 GAATTC HgaI 252 GCGTC GACGC(5/10) 4520 GCGTC Hin1I 151 GGCGCC G[GA]^CG[CT]C 4519 GGCGTC KspI 175 CCGCGG CCGC^GG 398 CCGCGG NcoI 1935 CCATGG C^CATGG 4486 CCATGG PflMI 3472 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3919 CCAAGACTTGG PpuMI 161 GGGTCCT [GA]G^G[AT]CC[CT] 2784 AGGTCCC PshAI 2981 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 3923 GACTTGGGTC RcaI 2779 TCATGA T^CATGA 3346 TCATGA SacII 175 CCGCGG CCGC^GG 398 CCGCGG ScaI 4265 AGTACT AGT^ACT 4659 AGTACT Van91I 3472 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3919 CCAAGACTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 114 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AseI AsnI Asp700I AvaI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsgI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DsaI EarI EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PleI PshBI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI