##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AclI AgeI Aor51HI
ApaI AscI Asp718I AspI AviII
AvrII BalI BbrPI BbvCI BlnI
BsaBI BsaI BsiEI BsiWI BsmBI
BspDI BsrFI BstBI BstEII BstPI
BstXI Cfr10I ClaI CpoI DrdI
EaeI EagI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRV FseI FspI
HpaI KpnI MamI MluI MluNI
MscI NaeI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SalI SbfI SexAI SfiI
SfuI SgrAI SnaBI Sse8387I SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccI 1204 GTATAC GT^[AC][GT]AC
AccIII 5082 TCCGGA T^CCGGA
AflII 440 CTTAAG C^TTAAG
AhdI 3044 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1493 GTGCAC G^TGCAC
ApaLI 1493 GTGCAC G^TGCAC
AspEI 3044 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 151 GGCGCC G^G[CT][GA]CC
BbeI 151 GGCGCC GGCGC^C
BciVI 2308 GTATCC GTATCC(6/5)
BclI 3250 TGATCA T^GATCA
BfrI 440 CTTAAG C^TTAAG
BglII 843 AGATCT A^GATCT
BsaAI 3836 CACGTA [CT]AC^GT[GA]
BseAI 5082 TCCGGA T^CCGGA
BspEI 5082 TCCGGA T^CCGGA
BssSI 5646 CTCGTG CACGAG(-5/-1)
Bst1107I 1204 GTATAC GTA^TAC
BstAPI 4448 GCAGAGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 1204 GTATAC GTA^TAC
Eam1105I 3044 GACCAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 3250 TGATCA T^GATCA
HindIII 4736 AAGCTT A^AGCTT
KasI 151 GGCGCC G^GCGCC
MfeI 873 CAATTG C^AATTG
MroI 5082 TCCGGA T^CCGGA
MunI 873 CAATTG C^AATTG
NarI 151 GGCGCC GG^CGCC
NdeI 4702 CATATG CA^TATG
NheI 1945 GCTAGC G^CTAGC
SacI 2137 GAGCTC GAGCT^C
SapI 2816 GAAGAGC GCTCTTC(1/4)
SfoI 151 GGCGCC GGC^GCC
SmaI 158 CCCGGG CCC^GGG
SpeI 3164 ACTAGT A^CTAGT
SphI 764 GCATGC GCATG^C
XbaI 4523 TCTAGA T^CTAGA
XcmI 867 CCAGAACAATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 158 CCCGGG C^CCGGG
XmaI 158 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AcyI 151 GGCGCC G[GA]^CG[CT]C
4519 GGCGTC
AocI 5228 CCTCAGG CC^T[ACGT]AGG
5814 CCTGAGG
AspHI 1493 GTGCAC G[AT]GC[AT]^C
2137 GAGCTC
BamHI 2941 GGATCC G^GATCC
3364 GGATCC
BcgI 24 GCAGAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
5118 GCAGAAGCCTCG
BglI 102 GCCCCCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
192 GCCGGGCGGGC
BlpI 247 GCTAAGC GC^T[ACGT]AGC
1442 GCTCAGC
Bpu1102I 247 GCTAAGC GC^T[ACGT]AGC
1442 GCTCAGC
BsaHI 151 GGCGCC G[GA]^CG[CT]C
4519 GGCGTC
BsiHKAI 1493 GTGCAC G[AT]GC[AT]^C
2137 GAGCTC
BspHI 2779 TCATGA T^CATGA
3346 TCATGA
BspLU11I 1429 ACATGT A^CATGT
1952 ACATGT
BsrBI 323 CCGCTC CCGCTC(-3/-3)
811 GAGCGG
BssHII 8 GCGCGC G^CGCGC
188 GCGCGC
Bsu36I 5228 CCTCAGG CC^T[ACGT]AGG
5814 CCTGAGG
CelII 247 GCTAAGC GC^T[ACGT]AGC
1442 GCTCAGC
DraII 161 GGGTCCT [GA]G^G[ACGT]CC[CT]
2784 AGGTCCC
DraIII 4530 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
4532 CACAGTGTG
Eco81I 5228 CCTCAGG CC^T[ACGT]AGG
5814 CCTGAGG
EcoO109I 161 GGGTCCT [GA]G^G[ACGT]CC[CT]
2784 AGGTCCC
EcoRI 781 GAATTC G^AATTC
5668 GAATTC
HgaI 252 GCGTC GACGC(5/10)
4520 GCGTC
Hin1I 151 GGCGCC G[GA]^CG[CT]C
4519 GGCGTC
KspI 175 CCGCGG CCGC^GG
398 CCGCGG
NcoI 1935 CCATGG C^CATGG
4486 CCATGG
PflMI 3472 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3919 CCAAGACTTGG
PpuMI 161 GGGTCCT [GA]G^G[AT]CC[CT]
2784 AGGTCCC
PshAI 2981 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3923 GACTTGGGTC
RcaI 2779 TCATGA T^CATGA
3346 TCATGA
SacII 175 CCGCGG CCGC^GG
398 CCGCGG
ScaI 4265 AGTACT AGT^ACT
4659 AGTACT
Van91I 3472 CCAGTGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3919 CCAAGACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AseI
AsnI Asp700I AvaI AvaII BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I Bsp1407I BspMI
BsrDI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraI DsaI
EarI EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PleI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI SspI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI