##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
Alw44I ApaLI AscI AseI AsnI
Asp718I BclI BfrI BseAI BsiWI
BspEI BspLU11I BssHII BssSI BstBI
CpoI DraIII EcoNI EcoRV FbaI
FseI KpnI MfeI MluI MroI
MunI NheI NruI NspV PacI
PaeR7I PinAI PmeI PpuMI PshBI
Psp1406I PvuI RsrII SalI SapI
SfiI SfuI SgrAI SnaBI SphI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AatII 1405 GACGTC GACGT^C
AhdI 4051 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1492 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2803 AGCGCT AGC^GCT
AspEI 4051 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2876 TGCGCA TGC^GCA
AvrII 13 CCTAGG C^CTAGG
BamHI 404 GGATCC G^GATCC
BanI 4382 GGCGCC G^G[CT][GA]CC
BbeI 4382 GGCGCC GGCGC^C
BbvCI 4025 CCTCAGC CCTCAGC(-5/-2)
BciVI 4219 GTATCC GTATCC(6/5)
BglI 348 GCCGGATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 524 AGATCT A^GATCT
BlnI 13 CCTAGG C^CTAGG
BlpI 1726 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1726 GCTGAGC GC^T[ACGT]AGC
BsaBI 2365 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 4813 GGTCTC GGTCTC(1/5)
BsaWI 243 TCCGGT [AT]^CCGG[AT]
BsiEI 340 CGGCCG CG[GA][CT]^CG
Bsp1407I 5361 TGTACA T^GTACA
BspDI 2624 ATCGAT AT^CGAT
BsrFI 153 GCCGGC [GA]^CCGG[CT]
BsrGI 5361 TGTACA T^GTACA
Bst1107I 3869 GTATAC GTA^TAC
BstEII 1803 GGTGACC G^GT[ACGT]ACC
BstPI 1803 GGTGACC G^GT[ACGT]ACC
BstZ17I 3869 GTATAC GTA^TAC
Bsu36I 1492 CCTGAGG CC^T[ACGT]AGG
CelII 1726 GCTGAGC GC^T[ACGT]AGC
Cfr10I 153 GCCGGC [GA]^CCGG[CT]
ClaI 2624 ATCGAT AT^CGAT
DraI 2482 TTTAAA TTT^AAA
DrdI 4145 GACAGAGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 340 CGGCCG C^GGCCG
Eam1105I 4051 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 340 CGGCCG C^GGCCG
Eco47III 2803 AGCGCT AGC^GCT
Eco52I 340 CGGCCG C^GGCCG
Eco81I 1492 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1803 GGTGACC G^GT[ACGT]ACC
EcoRI 3387 GAATTC G^AATTC
FspI 2876 TGCGCA TGC^GCA
HpaI 1945 GTTAAC GTT^AAC
KasI 4382 GGCGCC G^GCGCC
MamI 2365 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 153 GCCGGC GCC^GGC
NarI 4382 GGCGCC GG^CGCC
NcoI 4164 CCATGG C^CATGG
NdeI 1342 CATATG CA^TATG
NgoMIV 153 GCCGGC G^CCGGC
NotI 339 GCGGCCGC GC^GGCCGC
SbfI 3027 CCTGCAGG CCTGCA^GG
ScaI 493 AGTACT AGT^ACT
SfoI 4382 GGCGCC GGC^GCC
SmaI 204 CCCGGG CCC^GGG
SmlI 1220 CTCAAG C^T[CT][GA]AG
Sse8387I 3027 CCTGCAGG CCTGCA^GG
SspBI 5361 TGTACA T^GTACA
SspI 865 AATATT AAT^ATT
StuI 4398 AGGCCT AGG^CCT
XmaCI 204 CCCGGG C^CCGGG
XmaI 204 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 3869 GTATAC GT^[AC][GT]AC
4957 GTCTAC
ApaI 201 GGGCCC GGGCC^C
207 GGGCCC
AspI 4706 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
5131 GACTGGGTC
BalI 2111 TGGCCA TGG^CCA
4018 TGGCCA
BbrPI 437 CACGTG CAC^GTG
511 CACGTG
BcgI 922 CGAATGGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
68 GCACGGGTTTCG
BsaAI 437 CACGTG [CT]AC^GT[GA]
511 CACGTG
BstAPI 795 GCAGTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3030 GCAGGAGCTGC
DraII 200 GGGGCCC [GA]G^G[ACGT]CC[CT]
5034 AGGCCCC
EcoO109I 200 GGGGCCC [GA]G^G[ACGT]CC[CT]
5034 AGGCCCC
HincII 1945 GTTAAC GT[CT]^[GA]AC
2091 GTCAAC
HindII 1945 GTTAAC GT[CT]^[GA]AC
2091 GTCAAC
KspI 99 CCGCGG CCGC^GG
400 CCGCGG
MluNI 2111 TGGCCA TGG^CCA
4018 TGGCCA
MscI 2111 TGGCCA TGG^CCA
4018 TGGCCA
PflFI 4706 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
5131 GACTGGGTC
PmaCI 437 CACGTG CAC^GTG
511 CACGTG
PmlI 437 CACGTG CAC^GTG
511 CACGTG
PshAI 4001 GACTGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5342 GACTGGGGTC
SacI 89 GAGCTC GAGCT^C
1765 GAGCTC
SacII 99 CCGCGG CCGC^GG
400 CCGCGG
SpeI 2550 ACTAGT A^CTAGT
3748 ACTAGT
Tth111I 4706 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
5131 GACTGGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApoI
Asp700I AspHI AvaI AvaII BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EaeI EarI EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PflMI PleI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmnI