##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AcyI Aor51HI AscI
Asp718I AspI AvaI AviII AvrII
BamHI BbeI BcgI BclI BglII
BlnI BsaHI BsiWI BsoBI BssHII
Bst1107I BstBI BstZ17I CpoI DraIII
EagI EclXI Eco47III Eco52I EcoNI
EcoRV FbaI FseI FspI Hin1I
HincII HindII HpaI KasI KpnI
KspI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NotI NruI
NspV PacI PaeR7I PflFI PmeI
PvuI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccIII 115 TCCGGA T^CCGGA
AclI 3743 AACGTT AA^CGTT
AgeI 2017 ACCGGT A^CCGGT
Alw44I 4724 GTGCAC G^TGCAC
ApaI 3813 GGGCCC GGGCC^C
ApaLI 4724 GTGCAC G^TGCAC
AseI 3108 ATTAAT AT^TAAT
AsnI 3108 ATTAAT AT^TAAT
Asp700I 4504 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbvCI 1471 GCTGAGG CCTCAGC(-5/-2)
BglI 330 GCCGCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1450 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1450 GCTCAGC GC^T[ACGT]AGC
BsaBI 2509 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 115 TCCGGA T^CCGGA
BsiEI 146 CGGTCG CG[GA][CT]^CG
BsmBI 4091 CGTCTC CGTCTC(1/5)
Bsp1407I 3713 TGTACA T^GTACA
BspDI 467 ATCGAT AT^CGAT
BspEI 115 TCCGGA T^CCGGA
BspHI 1566 TCATGA T^CATGA
BsrBI 331 CCGCTC CCGCTC(-3/-3)
BsrGI 3713 TGTACA T^GTACA
BstAPI 43 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 929 CCAGCCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1450 GCTCAGC GC^T[ACGT]AGC
ClaI 467 ATCGAT AT^CGAT
EcoRI 1681 GAATTC G^AATTC
HgaI 4358 GCGTC GACGC(5/10)
MamI 2509 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 115 TCCGGA T^CCGGA
PinAI 2017 ACCGGT A^CCGGT
PpuMI 3018 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 3108 ATTAAT AT^TAAT
Psp1406I 3743 AACGTT AA^CGTT
PvuII 2656 CAGCTG CAG^CTG
RcaI 1566 TCATGA T^CATGA
SspBI 3713 TGTACA T^GTACA
StuI 844 AGGCCT AGG^CCT
XmnI 4504 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AccI 384 GTCTAC GT^[AC][GT]AC
4792 GTCTAC
AccII 3856 CGCG CG^CG
4199 CGCG
AflII 3348 CTTAAG C^TTAAG
3633 CTTAAG
AhdI 404 GACAGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3167 GACTGGCAGTC
AspEI 404 GACAGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3167 GACTGGCAGTC
BalI 355 TGGCCA TGG^CCA
3930 TGGCCA
BbrPI 1524 CACGTG CAC^GTG
1924 CACGTG
BfrI 3348 CTTAAG C^TTAAG
3633 CTTAAG
BsaAI 1524 CACGTG [CT]AC^GT[GA]
1924 CACGTG
BsaI 1256 GAGACC GGTCTC(1/5)
4440 GGTCTC
BsaWI 115 TCCGGA [AT]^CCGG[AT]
2017 ACCGGT
BsmI 2058 GAATGC GAATGC(1/-1)
4535 GAATGC
BspLU11I 2300 ACATGT A^CATGT
3642 ACATGT
BsrDI 205 CATTGC GCAATG(2/0)
3101 CATTGC
BstEII 4615 GGTAACC G^GT[ACGT]ACC
4679 GGTAACC
BstPI 4615 GGTAACC G^GT[ACGT]ACC
4679 GGTAACC
BstUI 3856 CGCG CG^CG
4199 CGCG
DsaI 438 CCATGG C^C[GA][CT]GG
1041 CCATGG
EaeI 355 TGGCCA [CT]^GGCC[GA]
3930 TGGCCA
Eam1105I 404 GACAGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3167 GACTGGCAGTC
EarI 2531 CTCTTC CTCTTC(1/4)
4063 CTCTTC
EcoO65I 4615 GGTAACC G^GT[ACGT]ACC
4679 GGTAACC
HaeII 1062 GGCGCT [GA]GCGC^[CT]
3324 AGCGCC
HindIII 350 AAGCTT A^AGCTT
2921 AAGCTT
Ksp632I 2531 CTCTTC CTCTTC(1/4)
4063 CTCTTC
MluNI 355 TGGCCA TGG^CCA
3930 TGGCCA
MscI 355 TGGCCA TGG^CCA
3930 TGGCCA
MspA1I 1099 CAGCGG C[AC]G^C[GT]G
2656 CAGCTG
MvnI 3856 CGCG CG^CG
4199 CGCG
NcoI 438 CCATGG C^CATGG
1041 CCATGG
NheI 1127 GCTAGC G^CTAGC
1771 GCTAGC
PmaCI 1524 CACGTG CAC^GTG
1924 CACGTG
PmlI 1524 CACGTG CAC^GTG
1924 CACGTG
PshAI 105 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4011 GACTCCTGTC
SacI 1739 GAGCTC GAGCT^C
3270 GAGCTC
ScaI 4293 AGTACT AGT^ACT
4426 AGTACT
SphI 183 GCATGC GCATG^C
4193 GCATGC
SspI 1854 AATATT AAT^ATT
2842 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI AspHI
AvaII BanI BanII BbsI BbvI
BciVI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DrdI Eco81I EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XbaI XcmI XhoII