################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII Acc65I AcyI Aor51HI AscI Asp718I AspI AvaI AviII AvrII BamHI BbeI BcgI BclI BglII BlnI BsaHI BsiWI BsoBI BssHII Bst1107I BstBI BstZ17I CpoI DraIII EagI EclXI Eco47III Eco52I EcoNI EcoRV FbaI FseI FspI Hin1I HincII HindII HpaI KasI KpnI KspI MfeI MluI MunI NaeI NarI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PmeI PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccIII 115 TCCGGA T^CCGGA AclI 3743 AACGTT AA^CGTT AgeI 2017 ACCGGT A^CCGGT Alw44I 4724 GTGCAC G^TGCAC ApaI 3813 GGGCCC GGGCC^C ApaLI 4724 GTGCAC G^TGCAC AseI 3108 ATTAAT AT^TAAT AsnI 3108 ATTAAT AT^TAAT Asp700I 4504 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbvCI 1471 GCTGAGG CCTCAGC(-5/-2) BglI 330 GCCGCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 1450 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1450 GCTCAGC GC^T[ACGT]AGC BsaBI 2509 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 115 TCCGGA T^CCGGA BsiEI 146 CGGTCG CG[GA][CT]^CG BsmBI 4091 CGTCTC CGTCTC(1/5) Bsp1407I 3713 TGTACA T^GTACA BspDI 467 ATCGAT AT^CGAT BspEI 115 TCCGGA T^CCGGA BspHI 1566 TCATGA T^CATGA BsrBI 331 CCGCTC CCGCTC(-3/-3) BsrGI 3713 TGTACA T^GTACA BstAPI 43 GCAGGCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 929 CCAGCCATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1450 GCTCAGC GC^T[ACGT]AGC ClaI 467 ATCGAT AT^CGAT EcoRI 1681 GAATTC G^AATTC HgaI 4358 GCGTC GACGC(5/10) MamI 2509 GATAGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 115 TCCGGA T^CCGGA PinAI 2017 ACCGGT A^CCGGT PpuMI 3018 GGGACCT [GA]G^G[AT]CC[CT] PshBI 3108 ATTAAT AT^TAAT Psp1406I 3743 AACGTT AA^CGTT PvuII 2656 CAGCTG CAG^CTG RcaI 1566 TCATGA T^CATGA SspBI 3713 TGTACA T^GTACA StuI 844 AGGCCT AGG^CCT XmnI 4504 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AccI 384 GTCTAC GT^[AC][GT]AC 4792 GTCTAC AccII 3856 CGCG CG^CG 4199 CGCG AflII 3348 CTTAAG C^TTAAG 3633 CTTAAG AhdI 404 GACAGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3167 GACTGGCAGTC AspEI 404 GACAGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3167 GACTGGCAGTC BalI 355 TGGCCA TGG^CCA 3930 TGGCCA BbrPI 1524 CACGTG CAC^GTG 1924 CACGTG BfrI 3348 CTTAAG C^TTAAG 3633 CTTAAG BsaAI 1524 CACGTG [CT]AC^GT[GA] 1924 CACGTG BsaI 1256 GAGACC GGTCTC(1/5) 4440 GGTCTC BsaWI 115 TCCGGA [AT]^CCGG[AT] 2017 ACCGGT BsmI 2058 GAATGC GAATGC(1/-1) 4535 GAATGC BspLU11I 2300 ACATGT A^CATGT 3642 ACATGT BsrDI 205 CATTGC GCAATG(2/0) 3101 CATTGC BstEII 4615 GGTAACC G^GT[ACGT]ACC 4679 GGTAACC BstPI 4615 GGTAACC G^GT[ACGT]ACC 4679 GGTAACC BstUI 3856 CGCG CG^CG 4199 CGCG DsaI 438 CCATGG C^C[GA][CT]GG 1041 CCATGG EaeI 355 TGGCCA [CT]^GGCC[GA] 3930 TGGCCA Eam1105I 404 GACAGCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3167 GACTGGCAGTC EarI 2531 CTCTTC CTCTTC(1/4) 4063 CTCTTC EcoO65I 4615 GGTAACC G^GT[ACGT]ACC 4679 GGTAACC HaeII 1062 GGCGCT [GA]GCGC^[CT] 3324 AGCGCC HindIII 350 AAGCTT A^AGCTT 2921 AAGCTT Ksp632I 2531 CTCTTC CTCTTC(1/4) 4063 CTCTTC MluNI 355 TGGCCA TGG^CCA 3930 TGGCCA MscI 355 TGGCCA TGG^CCA 3930 TGGCCA MspA1I 1099 CAGCGG C[AC]G^C[GT]G 2656 CAGCTG MvnI 3856 CGCG CG^CG 4199 CGCG NcoI 438 CCATGG C^CATGG 1041 CCATGG NheI 1127 GCTAGC G^CTAGC 1771 GCTAGC PmaCI 1524 CACGTG CAC^GTG 1924 CACGTG PmlI 1524 CACGTG CAC^GTG 1924 CACGTG PshAI 105 GACTCTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 4011 GACTCCTGTC SacI 1739 GAGCTC GAGCT^C 3270 GAGCTC ScaI 4293 AGTACT AGT^ACT 4426 AGTACT SphI 183 GCATGC GCATG^C 4193 GCATGC SspI 1854 AATATT AAT^ATT 2842 AATATT ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AvaII BanI BanII BbsI BbvI BciVI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DrdI Eco81I EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoII