##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Aor51HI AscI AseI AsnI Asp718I
AviII AvrII BbeI BcgI BclI
BlnI BsaBI BsaWI BseAI BsiEI
BsiWI BspDI BspEI BspHI BsrDI
BssHII BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I ClaI CpoI EagI
EclXI Eco47III Eco52I EcoT22I FbaI
FseI FspI HaeII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NarI NdeI NotI
NruI NsiI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspI SwaI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 2980 ACATGT A^C[GA][CT]GT
Alw44I 3780 GTGCAC G^TGCAC
ApaLI 3780 GTGCAC G^TGCAC
Asp700I 228 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbrPI 2873 CACGTG CAC^GTG
BlpI 1837 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1837 GCTGAGC GC^T[ACGT]AGC
BsaAI 2873 CACGTG [CT]AC^GT[GA]
BsmBI 1320 GAGACG CGTCTC(1/5)
Bsp1407I 3472 TGTACA T^GTACA
BspLU11I 2980 ACATGT A^CATGT
BsrFI 834 GCCGGC [GA]^CCGG[CT]
BsrGI 3472 TGTACA T^GTACA
CelII 1837 GCTGAGC GC^T[ACGT]AGC
Cfr10I 834 GCCGGC [GA]^CCGG[CT]
DraI 507 TTTAAA TTT^AAA
DraIII 801 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 567 GACCCAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 120 CCTGAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 747 GAATTC G^AATTC
NaeI 834 GCCGGC GCC^GGC
NgoMIV 834 GCCGGC G^CCGGC
NheI 1216 GCTAGC G^CTAGC
PmaCI 2873 CACGTG CAC^GTG
PmlI 2873 CACGTG CAC^GTG
PshAI 3165 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI 2225 ACCTGGT A^CC[AT]GGT
SspBI 3472 TGTACA T^GTACA
XbaI 910 TCTAGA T^CTAGA
XmnI 228 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 2660 GTAGAC GT^[AC][GT]AC
4233 GTAGAC
AflII 1992 CTTAAG C^TTAAG
3694 CTTAAG
AhdI 871 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3151 GACTCCTTGTC
AocI 1328 CCTTAGG CC^T[ACGT]AGG
1823 CCTCAGG
ApaI 3541 GGGCCC GGGCC^C
4281 GGGCCC
AspEI 871 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3151 GACTCCTTGTC
AspI 74 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1365 GACTTTGTC
BbvCI 1669 GCTGAGG CCTCAGC(-5/-2)
1729 GCTGAGG
BciVI 1425 GTATCC GTATCC(6/5)
1775 GTATCC
BfrI 1992 CTTAAG C^TTAAG
3694 CTTAAG
BsgI 1591 GTGCAG GTGCAG(16/14)
4077 GTGCAG
BspMI 2130 ACCTGC ACCTGC(4/8)
2237 ACCTGC
BsrBI 2286 CCGCTC CCGCTC(-3/-3)
3623 GAGCGG
BstEII 260 GGTCACC G^GT[ACGT]ACC
1560 GGTGACC
BstPI 260 GGTCACC G^GT[ACGT]ACC
1560 GGTGACC
Bsu36I 1328 CCTTAGG CC^T[ACGT]AGG
1823 CCTCAGG
Eam1105I 871 GACAAGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3151 GACTCCTTGTC
Eco81I 1328 CCTTAGG CC^T[ACGT]AGG
1823 CCTCAGG
EcoO65I 260 GGTCACC G^GT[ACGT]ACC
1560 GGTGACC
HindIII 102 AAGCTT A^AGCTT
3697 AAGCTT
NspI 983 ACATGC [GA]CATG^[CT]
2980 ACATGT
PaeR7I 2189 CTCGAG C^TCGAG
2402 CTCGAG
PflFI 74 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1365 GACTTTGTC
SacI 1840 GAGCTC GAGCT^C
4086 GAGCTC
SapI 1648 GAAGAGC GCTCTTC(1/4)
201 GCTCTTC
SmaI 324 CCCGGG CCC^GGG
3225 CCCGGG
Tth111I 74 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1365 GACTTTGTC
XhoI 2189 CTCGAG C^TCGAG
2402 CTCGAG
XmaCI 324 CCCGGG C^CCGGG
3225 CCCGGG
XmaI 324 CCCGGG C^CCGGG
3225 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvI BcnI BfaI
BglI BglII BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoRV
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII