##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AclI AflII AocI Aor51HI AscI
AseI AsnI AviII BfrI BlpI
Bpu1102I BsaBI BsiWI BspDI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I FseI FspI MamI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PmeI PshBI
Psp1406I PvuI RsrII ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
SwaI XcmI XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 1012 GGTACC G^GTACC
AhdI 836 GACTTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 6158 GGGCCC GGGCC^C
Asp700I 5088 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1012 GGTACC G^GTACC
AspEI 836 GACTTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1039 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 5726 GGATCC G^GATCC
BbeI 730 GGCGCC GGCGC^C
BcgI 4685 CGACCCTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 424 TGATCA T^GATCA
BsiEI 4504 CGGTCG CG[GA][CT]^CG
BsmI 3819 GCATTC GAATGC(1/-1)
Bsp1407I 1956 TGTACA T^GTACA
BspHI 476 TCATGA T^CATGA
BspLU11I 3102 ACATGT A^CATGT
BsrBI 5394 GAGCGG CCGCTC(-3/-3)
BsrGI 1956 TGTACA T^GTACA
BstXI 2896 CCACATCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eam1105I 836 GACTTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 2057 GATATC GAT^ATC
FbaI 424 TGATCA T^GATCA
HgaI 64 GCGTC GACGC(5/10)
KasI 730 GGCGCC G^GCGCC
KpnI 1012 GGTACC GGTAC^C
KspI 3022 CCGCGG CCGC^GG
MfeI 2631 CAATTG C^AATTG
MluI 4602 ACGCGT A^CGCGT
MunI 2631 CAATTG C^AATTG
NarI 730 GGCGCC GG^CGCC
PflFI 1039 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3964 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 619 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 1105 GACTGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 476 TCATGA T^CATGA
SacII 3022 CCGCGG CCGC^GG
SbfI 858 CCTGCAGG CCTGCA^GG
SfoI 730 GGCGCC GGC^GCC
SmaI 330 CCCGGG CCC^GGG
SphI 4894 GCATGC GCATG^C
Sse8387I 858 CCTGCAGG CCTGCA^GG
SspBI 1956 TGTACA T^GTACA
Tth111I 1039 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3964 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 330 CCCGGG C^CCGGG
XmaI 330 CCCGGG C^CCGGG
XmnI 5088 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AatII 841 GACGTC GACGT^C
1109 GACGTC
AccIII 847 TCCGGA T^CCGGA
4619 TCCGGA
AgeI 821 ACCGGT A^CCGGT
1009 ACCGGT
Alw44I 1280 GTGCAC G^TGCAC
3279 GTGCAC
ApaLI 1280 GTGCAC G^TGCAC
3279 GTGCAC
AvrII 414 CCTAGG C^CTAGG
4568 CCTAGG
BciVI 1494 GGATAC GTATCC(6/5)
2331 GTATCC
BglI 4753 GCCAGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5022 GCCTCGCTGGC
BglII 2557 AGATCT A^GATCT
5928 AGATCT
BlnI 414 CCTAGG C^CTAGG
4568 CCTAGG
BseAI 847 TCCGGA T^CCGGA
4619 TCCGGA
BsmBI 535 CGTCTC CGTCTC(1/5)
843 CGTCTC
BspEI 847 TCCGGA T^CCGGA
4619 TCCGGA
BsrDI 1760 GCAATG GCAATG(2/0)
3877 GCAATG
BsrFI 821 ACCGGT [GA]^CCGG[CT]
1009 ACCGGT
BstAPI 1080 GCAGATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3233 GCAGGAGCTGC
Cfr10I 821 ACCGGT [GA]^CCGG[CT]
1009 ACCGGT
DraIII 2323 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
5415 CACTACGTG
EcoRI 2855 GAATTC G^AATTC
5556 GAATTC
EcoT22I 5290 ATGCAT ATGCA^T
5525 ATGCAT
HaeII 730 GGCGCC [GA]GCGC^[CT]
4761 GGCGCT
HindIII 3178 AAGCTT A^AGCTT
3455 AAGCTT
HpaI 2976 GTTAAC GTT^AAC
6353 GTTAAC
MroI 847 TCCGGA T^CCGGA
4619 TCCGGA
NcoI 3293 CCATGG C^CATGG
4932 CCATGG
NsiI 5290 ATGCAT ATGCA^T
5525 ATGCAT
PinAI 821 ACCGGT A^CCGGT
1009 ACCGGT
SacI 1132 GAGCTC GAGCT^C
6363 GAGCTC
XbaI 3707 TCTAGA T^CTAGA
3839 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbrPI BbsI BbvCI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DrdI
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PmaCI
PmlI PstI PvuII RsaI SalI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII