################################## # NO CUT ENZYMES ( 58 enzymes) # ################################## AclI AflII AocI Aor51HI AscI AseI AsnI AviII BfrI BlpI Bpu1102I BsaBI BsiWI BspDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI EagI EclXI Eco47III Eco52I Eco81I EcoO65I FseI FspI MamI NaeI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PmeI PshBI Psp1406I PvuI RsrII ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SwaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 1012 GGTACC G^GTACC AhdI 836 GACTTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 6158 GGGCCC GGGCC^C Asp700I 5088 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1012 GGTACC G^GTACC AspEI 836 GACTTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1039 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC BamHI 5726 GGATCC G^GATCC BbeI 730 GGCGCC GGCGC^C BcgI 4685 CGACCCTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 424 TGATCA T^GATCA BsiEI 4504 CGGTCG CG[GA][CT]^CG BsmI 3819 GCATTC GAATGC(1/-1) Bsp1407I 1956 TGTACA T^GTACA BspHI 476 TCATGA T^CATGA BspLU11I 3102 ACATGT A^CATGT BsrBI 5394 GAGCGG CCGCTC(-3/-3) BsrGI 1956 TGTACA T^GTACA BstXI 2896 CCACATCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Eam1105I 836 GACTTGACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRV 2057 GATATC GAT^ATC FbaI 424 TGATCA T^GATCA HgaI 64 GCGTC GACGC(5/10) KasI 730 GGCGCC G^GCGCC KpnI 1012 GGTACC GGTAC^C KspI 3022 CCGCGG CCGC^GG MfeI 2631 CAATTG C^AATTG MluI 4602 ACGCGT A^CGCGT MunI 2631 CAATTG C^AATTG NarI 730 GGCGCC GG^CGCC PflFI 1039 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 3964 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 619 AGGACCC [GA]G^G[AT]CC[CT] PshAI 1105 GACTGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 476 TCATGA T^CATGA SacII 3022 CCGCGG CCGC^GG SbfI 858 CCTGCAGG CCTGCA^GG SfoI 730 GGCGCC GGC^GCC SmaI 330 CCCGGG CCC^GGG SphI 4894 GCATGC GCATG^C Sse8387I 858 CCTGCAGG CCTGCA^GG SspBI 1956 TGTACA T^GTACA Tth111I 1039 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 3964 CCACAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 330 CCCGGG C^CCGGG XmaI 330 CCCGGG C^CCGGG XmnI 5088 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AatII 841 GACGTC GACGT^C 1109 GACGTC AccIII 847 TCCGGA T^CCGGA 4619 TCCGGA AgeI 821 ACCGGT A^CCGGT 1009 ACCGGT Alw44I 1280 GTGCAC G^TGCAC 3279 GTGCAC ApaLI 1280 GTGCAC G^TGCAC 3279 GTGCAC AvrII 414 CCTAGG C^CTAGG 4568 CCTAGG BciVI 1494 GGATAC GTATCC(6/5) 2331 GTATCC BglI 4753 GCCAGGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 5022 GCCTCGCTGGC BglII 2557 AGATCT A^GATCT 5928 AGATCT BlnI 414 CCTAGG C^CTAGG 4568 CCTAGG BseAI 847 TCCGGA T^CCGGA 4619 TCCGGA BsmBI 535 CGTCTC CGTCTC(1/5) 843 CGTCTC BspEI 847 TCCGGA T^CCGGA 4619 TCCGGA BsrDI 1760 GCAATG GCAATG(2/0) 3877 GCAATG BsrFI 821 ACCGGT [GA]^CCGG[CT] 1009 ACCGGT BstAPI 1080 GCAGATTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3233 GCAGGAGCTGC Cfr10I 821 ACCGGT [GA]^CCGG[CT] 1009 ACCGGT DraIII 2323 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG 5415 CACTACGTG EcoRI 2855 GAATTC G^AATTC 5556 GAATTC EcoT22I 5290 ATGCAT ATGCA^T 5525 ATGCAT HaeII 730 GGCGCC [GA]GCGC^[CT] 4761 GGCGCT HindIII 3178 AAGCTT A^AGCTT 3455 AAGCTT HpaI 2976 GTTAAC GTT^AAC 6353 GTTAAC MroI 847 TCCGGA T^CCGGA 4619 TCCGGA NcoI 3293 CCATGG C^CATGG 4932 CCATGG NsiI 5290 ATGCAT ATGCA^T 5525 ATGCAT PinAI 821 ACCGGT A^CCGGT 1009 ACCGGT SacI 1132 GAGCTC GAGCT^C 6363 GAGCTC XbaI 3707 TCTAGA T^CTAGA 3839 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanI BanII BbrPI BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DrdI DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PmaCI PmlI PstI PvuII RsaI SalI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII