##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
Acc65I AccI AccII AccIII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AviII AvrII BamHI BbeI
BbrPI BcnI BglI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BsrBI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I ClaI
CpoI DraIII EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV FseI
FspI HaeII HgaI KasI KpnI
KspI MamI MluI MroI MvnI
NaeI NarI NciI NgoMIV NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PvuI
RsrII SacII SalI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SphI SspBI StuI SwaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AatII 4457 GACGTC GACGT^C
AclI 4334 AACGTT AA^CGTT
AcyI 4457 GACGTC G[GA]^CG[CT]C
AflII 215 CTTAAG C^TTAAG
AhdI 5152 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 3663 ATTAAT AT^TAAT
AsnI 3663 ATTAAT AT^TAAT
AspEI 5152 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4725 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BciVI 5107 GGATAC GTATCC(6/5)
BfrI 215 CTTAAG C^TTAAG
BsaHI 4457 GACGTC G[GA]^CG[CT]C
BspHI 927 TCATGA T^CATGA
BssSI 1994 CTCGTG CACGAG(-5/-1)
BstXI 2984 CCATCTATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 1870 GACTTTTTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 47 CCATGG C^C[GA][CT]GG
Eam1105I 5152 GACAGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 23 GAATTC G^AATTC
EcoT22I 886 ATGCAT ATGCA^T
Hin1I 4457 GACGTC G[GA]^CG[CT]C
HindIII 3099 AAGCTT A^AGCTT
MfeI 3601 CAATTG C^AATTG
MunI 3601 CAATTG C^AATTG
NcoI 47 CCATGG C^CATGG
NdeI 817 CATATG CA^TATG
NheI 3502 GCTAGC G^CTAGC
NsiI 886 ATGCAT ATGCA^T
PflFI 4725 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3663 ATTAAT AT^TAAT
Psp1406I 4334 AACGTT AA^CGTT
RcaI 927 TCATGA T^CATGA
ScaI 744 AGTACT AGT^ACT
SpeI 2373 ACTAGT A^CTAGT
SspI 2031 AATATT AAT^ATT
Tth111I 4725 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2037 TCTAGA T^CTAGA
XcmI 2351 CCAAAAAATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AflIII 99 ACATGT A^C[GA][CT]GT
1277 ACATGT
AocI 1311 CCTGAGG CC^T[ACGT]AGG
5272 CCTGAGG
AvaI 2813 CCCGAG C^[CT]CG[GA]G
4606 CTCGGG
BalI 76 TGGCCA TGG^CCA
821 TGGCCA
BcgI 4037 CGACAGGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4018 GCAAGAAGGTCG
BclI 34 TGATCA T^GATCA
4399 TGATCA
BlpI 672 GCTCAGC GC^T[ACGT]AGC
3396 GCTCAGC
Bpu1102I 672 GCTCAGC GC^T[ACGT]AGC
3396 GCTCAGC
BsaI 1261 GAGACC GGTCTC(1/5)
3835 GGTCTC
BsmI 884 GAATGC GAATGC(1/-1)
2704 GAATGC
BsoBI 2813 CCCGAG C^[CT]CG[GA]G
4606 CTCGGG
BspLU11I 99 ACATGT A^CATGT
1277 ACATGT
BsrFI 3870 GCCGGT [GA]^CCGG[CT]
5070 ACCGGC
BstAPI 2699 GCATTGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5224 GCAGGAGGTGC
Bsu36I 1311 CCTGAGG CC^T[ACGT]AGG
5272 CCTGAGG
CelII 672 GCTCAGC GC^T[ACGT]AGC
3396 GCTCAGC
CfoI 3945 GCGC GCG^C
4137 GCGC
Cfr10I 3870 GCCGGT [GA]^CCGG[CT]
5070 ACCGGC
EaeI 76 TGGCCA [CT]^GGCC[GA]
821 TGGCCA
Eco81I 1311 CCTGAGG CC^T[ACGT]AGG
5272 CCTGAGG
FbaI 34 TGATCA T^GATCA
4399 TGATCA
HapII 3871 CCGG C^CGG
5071 CCGG
HhaI 3945 GCGC GCG^C
4137 GCGC
HinP1I 3945 GCGC G^CGC
4137 GCGC
HincII 989 GTTAAC GT[CT]^[GA]AC
1381 GTTAAC
HindII 989 GTTAAC GT[CT]^[GA]AC
1381 GTTAAC
HpaI 989 GTTAAC GTT^AAC
1381 GTTAAC
HpaII 3871 CCGG C^CGG
5071 CCGG
MluNI 76 TGGCCA TGG^CCA
821 TGGCCA
MscI 76 TGGCCA TGG^CCA
821 TGGCCA
MspI 3871 CCGG C^CGG
5071 CCGG
PpuMI 1023 GGGACCC [GA]G^G[AT]CC[CT]
5049 AGGTCCT
PvuII 3958 CAGCTG CAG^CTG
4298 CAGCTG
SacI 767 GAGCTC GAGCT^C
4324 GAGCTC
SapI 4709 GAAGAGC GCTCTTC(1/4)
5208 GAAGAGC
SbfI 2189 CCTGCAGG CCTGCA^GG
5201 CCTGCAGG
Sse8387I 2189 CCTGCAGG CCTGCA^GG
5201 CCTGCAGG
TaqI 3688 TCGA T^CGA
4036 TCGA
TthHB8I 3688 TCGA T^CGA
4036 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI Asp700I AspHI AvaII
BanI BanII BbsI BbvCI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XhoII XmnI