##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AccI AccIII AgeI Alw44I ApaLI
AscI BamHI BbrPI BglII BsaBI
BsaWI BseAI BsiWI BspDI BspEI
BssHII Bst1107I BstBI BstXI BstYI
BstZ17I ClaI CpoI EagI EclXI
Eco52I EcoRI EcoRV FseI HindIII
KspI MamI MfeI MflI MluI
MroI MunI NaeI NdeI NgoMIV
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PvuI RsrII SacII SalI SbfI
SfiI SfuI SgrAI SnaBI Sse8387I
Van91I XcmI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AatII 119 GACGTC GACGT^C
Acc65I 4500 GGTACC G^GTACC
AclI 441 AACGTT AA^CGTT
AflII 1860 CTTAAG C^TTAAG
Aor51HI 2425 AGCGCT AGC^GCT
ApaI 125 GGGCCC GGGCC^C
Asp718I 4500 GGTACC G^GTACC
AspI 4176 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2545 TGCGCA TGC^GCA
AvrII 111 CCTAGG C^CTAGG
BbeI 61 GGCGCC GGCGC^C
BcgI 1594 CGAGAGACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2110 TGATCA T^GATCA
BfrI 1860 CTTAAG C^TTAAG
BlnI 111 CCTAGG C^CTAGG
BsaAI 3015 CACGTA [CT]AC^GT[GA]
BsiEI 130 CGGTCG CG[GA][CT]^CG
BsmBI 1546 GAGACG CGTCTC(1/5)
BsmI 4804 GCATTC GAATGC(1/-1)
Bsp1407I 4348 TGTACA T^GTACA
BspHI 3377 TCATGA T^CATGA
BsrFI 2913 ACCGGC [GA]^CCGG[CT]
BsrGI 4348 TGTACA T^GTACA
BssSI 2421 CACGAG CACGAG(-5/-1)
Cfr10I 2913 ACCGGC [GA]^CCGG[CT]
DraIII 5079 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2425 AGCGCT AGC^GCT
EcoT22I 5049 ATGCAT ATGCA^T
FbaI 2110 TGATCA T^GATCA
FspI 2545 TGCGCA TGC^GCA
HpaI 1899 GTTAAC GTT^AAC
KasI 61 GGCGCC G^GCGCC
KpnI 4500 GGTACC GGTAC^C
NarI 61 GGCGCC GG^CGCC
NcoI 4815 CCATGG C^CATGG
NheI 4230 GCTAGC G^CTAGC
NsiI 5049 ATGCAT ATGCA^T
PflFI 4176 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 441 AACGTT AA^CGTT
RcaI 3377 TCATGA T^CATGA
SacI 322 GAGCTC GAGCT^C
SapI 79 GAAGAGC GCTCTTC(1/4)
SexAI 2581 ACCAGGT A^CC[AT]GGT
SfoI 61 GGCGCC GGC^GCC
SmaI 718 CCCGGG CCC^GGG
SpeI 1199 ACTAGT A^CTAGT
SphI 2542 GCATGC GCATG^C
SspBI 4348 TGTACA T^GTACA
SwaI 1841 ATTTAAAT ATTT^AAAT
TaqI 1332 TCGA T^CGA
Tth111I 4176 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1332 TCGA T^CGA
XmaCI 718 CCCGGG C^CCGGG
XmaI 718 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcyI 61 GGCGCC G[GA]^CG[CT]C
119 GACGTC
AhdI 1671 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1979 GACACACTGTC
AlwI 879 GATCC GGATC(4/5)
1287 GGATC
AseI 3533 ATTAAT AT^TAAT
5019 ATTAAT
AsnI 3533 ATTAAT AT^TAAT
5019 ATTAAT
AspEI 1671 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1979 GACACACTGTC
BciVI 2726 GGATAC GTATCC(6/5)
3883 GTATCC
BlpI 2316 GCTAAGC GC^T[ACGT]AGC
2388 GCTAAGC
Bpu1102I 2316 GCTAAGC GC^T[ACGT]AGC
2388 GCTAAGC
BsaHI 61 GGCGCC G[GA]^CG[CT]C
119 GACGTC
BspMI 1600 ACCTGC ACCTGC(4/8)
4615 GCAGGT
BsrDI 1854 CATTGC GCAATG(2/0)
5242 CATTGC
BstEII 1678 GGTCACC G^GT[ACGT]ACC
3757 GGTAACC
BstPI 1678 GGTCACC G^GT[ACGT]ACC
3757 GGTAACC
CelII 2316 GCTAAGC GC^T[ACGT]AGC
2388 GCTAAGC
DrdI 3049 GACTCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3068 GACCCACATGTC
DsaI 1020 CCACGG C^C[GA][CT]GG
4815 CCATGG
Eam1105I 1671 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1979 GACACACTGTC
EcoNI 65 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
254 CCTCAATGAGG
EcoO65I 1678 GGTCACC G^GT[ACGT]ACC
3757 GGTAACC
HgaI 429 GACGC GACGC(5/10)
1548 GACGC
Hin1I 61 GGCGCC G[GA]^CG[CT]C
119 GACGTC
HincII 1439 GTCAAC GT[CT]^[GA]AC
1899 GTTAAC
HindII 1439 GTCAAC GT[CT]^[GA]AC
1899 GTTAAC
PshAI 1938 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2796 GACTTGAGTC
PshBI 3533 ATTAAT AT^TAAT
5019 ATTAAT
PstI 835 CTGCAG CTGCA^G
2459 CTGCAG
ScaI 355 AGTACT AGT^ACT
4387 AGTACT
SspI 3466 AATATT AAT^ATT
4663 AATATT
XbaI 2405 TCTAGA T^CTAGA
4286 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwNI AocI ApoI Asp700I
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspLU11I BsrBI BsrI BssKI
BstAPI BstF5I BstNI BstUI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XmnI