################################## # NO CUT ENZYMES ( 64 enzymes) # ################################## AccI AccIII AgeI Alw44I ApaLI AscI BamHI BbrPI BglII BsaBI BsaWI BseAI BsiWI BspDI BspEI BssHII Bst1107I BstBI BstXI BstYI BstZ17I ClaI CpoI EagI EclXI Eco52I EcoRI EcoRV FseI HindIII KspI MamI MfeI MflI MluI MroI MunI NaeI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PvuI RsrII SacII SalI SbfI SfiI SfuI SgrAI SnaBI Sse8387I Van91I XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AatII 119 GACGTC GACGT^C Acc65I 4500 GGTACC G^GTACC AclI 441 AACGTT AA^CGTT AflII 1860 CTTAAG C^TTAAG Aor51HI 2425 AGCGCT AGC^GCT ApaI 125 GGGCCC GGGCC^C Asp718I 4500 GGTACC G^GTACC AspI 4176 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2545 TGCGCA TGC^GCA AvrII 111 CCTAGG C^CTAGG BbeI 61 GGCGCC GGCGC^C BcgI 1594 CGAGAGACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2110 TGATCA T^GATCA BfrI 1860 CTTAAG C^TTAAG BlnI 111 CCTAGG C^CTAGG BsaAI 3015 CACGTA [CT]AC^GT[GA] BsiEI 130 CGGTCG CG[GA][CT]^CG BsmBI 1546 GAGACG CGTCTC(1/5) BsmI 4804 GCATTC GAATGC(1/-1) Bsp1407I 4348 TGTACA T^GTACA BspHI 3377 TCATGA T^CATGA BsrFI 2913 ACCGGC [GA]^CCGG[CT] BsrGI 4348 TGTACA T^GTACA BssSI 2421 CACGAG CACGAG(-5/-1) Cfr10I 2913 ACCGGC [GA]^CCGG[CT] DraIII 5079 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 2425 AGCGCT AGC^GCT EcoT22I 5049 ATGCAT ATGCA^T FbaI 2110 TGATCA T^GATCA FspI 2545 TGCGCA TGC^GCA HpaI 1899 GTTAAC GTT^AAC KasI 61 GGCGCC G^GCGCC KpnI 4500 GGTACC GGTAC^C NarI 61 GGCGCC GG^CGCC NcoI 4815 CCATGG C^CATGG NheI 4230 GCTAGC G^CTAGC NsiI 5049 ATGCAT ATGCA^T PflFI 4176 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 441 AACGTT AA^CGTT RcaI 3377 TCATGA T^CATGA SacI 322 GAGCTC GAGCT^C SapI 79 GAAGAGC GCTCTTC(1/4) SexAI 2581 ACCAGGT A^CC[AT]GGT SfoI 61 GGCGCC GGC^GCC SmaI 718 CCCGGG CCC^GGG SpeI 1199 ACTAGT A^CTAGT SphI 2542 GCATGC GCATG^C SspBI 4348 TGTACA T^GTACA SwaI 1841 ATTTAAAT ATTT^AAAT TaqI 1332 TCGA T^CGA Tth111I 4176 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1332 TCGA T^CGA XmaCI 718 CCCGGG C^CCGGG XmaI 718 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AcyI 61 GGCGCC G[GA]^CG[CT]C 119 GACGTC AhdI 1671 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1979 GACACACTGTC AlwI 879 GATCC GGATC(4/5) 1287 GGATC AseI 3533 ATTAAT AT^TAAT 5019 ATTAAT AsnI 3533 ATTAAT AT^TAAT 5019 ATTAAT AspEI 1671 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1979 GACACACTGTC BciVI 2726 GGATAC GTATCC(6/5) 3883 GTATCC BlpI 2316 GCTAAGC GC^T[ACGT]AGC 2388 GCTAAGC Bpu1102I 2316 GCTAAGC GC^T[ACGT]AGC 2388 GCTAAGC BsaHI 61 GGCGCC G[GA]^CG[CT]C 119 GACGTC BspMI 1600 ACCTGC ACCTGC(4/8) 4615 GCAGGT BsrDI 1854 CATTGC GCAATG(2/0) 5242 CATTGC BstEII 1678 GGTCACC G^GT[ACGT]ACC 3757 GGTAACC BstPI 1678 GGTCACC G^GT[ACGT]ACC 3757 GGTAACC CelII 2316 GCTAAGC GC^T[ACGT]AGC 2388 GCTAAGC DrdI 3049 GACTCTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3068 GACCCACATGTC DsaI 1020 CCACGG C^C[GA][CT]GG 4815 CCATGG Eam1105I 1671 GACAGAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1979 GACACACTGTC EcoNI 65 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 254 CCTCAATGAGG EcoO65I 1678 GGTCACC G^GT[ACGT]ACC 3757 GGTAACC HgaI 429 GACGC GACGC(5/10) 1548 GACGC Hin1I 61 GGCGCC G[GA]^CG[CT]C 119 GACGTC HincII 1439 GTCAAC GT[CT]^[GA]AC 1899 GTTAAC HindII 1439 GTCAAC GT[CT]^[GA]AC 1899 GTTAAC PshAI 1938 GACATCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2796 GACTTGAGTC PshBI 3533 ATTAAT AT^TAAT 5019 ATTAAT PstI 835 CTGCAG CTGCA^G 2459 CTGCAG ScaI 355 AGTACT AGT^ACT 4387 AGTACT SspI 3466 AATATT AAT^ATT 4663 AATATT XbaI 2405 TCTAGA T^CTAGA 4286 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwNI AocI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BsrBI BsrI BssKI BstAPI BstF5I BstNI BstUI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XmnI