##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
Acc65I AclI AgeI AhdI Aor51HI
AscI Asp718I AspEI AvaI AviII
AvrII BbeI BbrPI BciVI BglI
BlnI BsiEI BsiWI BsmBI BsoBI
BspLU11I BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I Cfr10I
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I FseI FspI
HaeII HgaI HindIII KasI KpnI
KspI MfeI MluI MunI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
StuI SwaI Van91I XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 3150 GACGTC GACGT^C
AccIII 3160 TCCGGA T^CCGGA
AcyI 3150 GACGTC G[GA]^CG[CT]C
AflIII 2336 ACACGT A^C[GA][CT]GT
AseI 4462 ATTAAT AT^TAAT
AsnI 4462 ATTAAT AT^TAAT
BbvCI 2608 CCTCAGC CCTCAGC(-5/-2)
BclI 1059 TGATCA T^GATCA
BglII 1176 AGATCT A^GATCT
BlpI 179 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 179 GCTCAGC GC^T[ACGT]AGC
BsaBI 2675 GATTAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3150 GACGTC G[GA]^CG[CT]C
BseAI 3160 TCCGGA T^CCGGA
Bsp1407I 2249 TGTACA T^GTACA
BspEI 3160 TCCGGA T^CCGGA
BsrDI 2188 CATTGC GCAATG(2/0)
BsrGI 2249 TGTACA T^GTACA
BstAPI 4723 GCAGGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 5173 CCAACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 179 GCTCAGC GC^T[ACGT]AGC
EcoNI 4508 CCTCAAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1524 GAATTC G^AATTC
EcoRV 2179 GATATC GAT^ATC
FbaI 1059 TGATCA T^GATCA
Hin1I 3150 GACGTC G[GA]^CG[CT]C
HpaI 5205 GTTAAC GTT^AAC
MamI 2675 GATTAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3160 TCCGGA T^CCGGA
NdeI 402 CATATG CA^TATG
PshAI 3657 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4462 ATTAAT AT^TAAT
SacI 2422 GAGCTC GAGCT^C
SapI 2220 GAAGAGC GCTCTTC(1/4)
ScaI 2462 AGTACT AGT^ACT
SphI 4695 GCATGC GCATG^C
SspBI 2249 TGTACA T^GTACA
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 491 GTAGAC GT^[AC][GT]AC
3547 GTAGAC
AccII 82 CGCG CG^CG
146 CGCG
AflII 440 CTTAAG C^TTAAG
3221 CTTAAG
Alw44I 2516 GTGCAC G^TGCAC
4289 GTGCAC
ApaI 1859 GGGCCC GGGCC^C
2172 GGGCCC
ApaLI 2516 GTGCAC G^TGCAC
4289 GTGCAC
Asp700I 2539 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5127 GAATACATTC
AspI 3147 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
5060 GACACGGTC
BalI 1959 TGGCCA TGG^CCA
2274 TGGCCA
BamHI 1475 GGATCC G^GATCC
3157 GGATCC
BanI 1101 GGCACC G^G[CT][GA]CC
5023 GGCACC
BfrI 440 CTTAAG C^TTAAG
3221 CTTAAG
BsaAI 2337 CACGTA [CT]AC^GT[GA]
3544 CACGTA
BsaWI 2418 ACCGGA [AT]^CCGG[AT]
3160 TCCGGA
BspDI 782 ATCGAT AT^CGAT
3111 ATCGAT
BsrBI 2144 CCGCTC CCGCTC(-3/-3)
149 GAGCGG
BstUI 82 CGCG CG^CG
146 CGCG
CfoI 81 GCGC GCG^C
4316 GCGC
ClaI 782 ATCGAT AT^CGAT
3111 ATCGAT
DraIII 1330 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
1752 CACAGTGTG
EaeI 1959 TGGCCA [CT]^GGCC[GA]
2274 TGGCCA
HhaI 81 GCGC GCG^C
4316 GCGC
HinP1I 81 GCGC G^CGC
4316 GCGC
MluNI 1959 TGGCCA TGG^CCA
2274 TGGCCA
MscI 1959 TGGCCA TGG^CCA
2274 TGGCCA
MvnI 82 CGCG CG^CG
146 CGCG
NcoI 2285 CCATGG C^CATGG
3980 CCATGG
PflFI 3147 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
5060 GACACGGTC
PpuMI 34 GGGACCC [GA]G^G[AT]CC[CT]
4856 AGGACCC
SbfI 133 CCTGCAGG CCTGCA^GG
270 CCTGCAGG
SpeI 4218 ACTAGT A^CTAGT
4613 ACTAGT
Sse8387I 133 CCTGCAGG CCTGCA^GG
270 CCTGCAGG
TaqI 783 TCGA T^CGA
3112 TCGA
Tth111I 3147 GACGACGTC GAC[ACGT]^[ACGT][ACGT]GTC
5060 GACACGGTC
TthHB8I 783 TCGA T^CGA
3112 TCGA
XbaI 943 TCTAGA T^CTAGA
2575 TCTAGA
XmnI 2539 GAAACAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5127 GAATACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI AocI ApoI AspHI AvaII
BanII BbsI BbvI BcgI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspHI BspMI BsrI BssKI BstF5I
BstNI BstYI Bsu36I Cac8I Cfr13I
DdeI DpnII DraI DraII DsaI
EarI Eco81I EcoO109I EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PleI PstI PvuII
RcaI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII