##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII AclI AflII AgeI Alw44I
ApaI ApaLI AscI AseI AsnI
BcgI BclI BfrI BglI BsaBI
BsiWI BsmBI Bsp1407I BspDI BspHI
BspLU11I BsrFI BsrGI BssSI Bst1107I
BstBI BstZ17I Cfr10I ClaI CpoI
DraI EagI EclXI Eco52I EcoRV
FbaI FseI HindIII HpaI KspI
MamI MluI NaeI NgoMIV NheI
NotI NruI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI ScaI
SfiI SfuI SgrAI SnaBI SpeI
SphI SspBI SspI SwaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 2520 GGTACC G^GTACC
AccIII 2822 TCCGGA T^CCGGA
AhdI 3356 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 78 AGCGCT AGC^GCT
Asp718I 2520 GGTACC G^GTACC
AspEI 3356 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 141 CCTAGG C^CTAGG
BamHI 2307 GGATCC G^GATCC
BbeI 442 GGCGCC GGCGC^C
BglII 692 AGATCT A^GATCT
BlnI 141 CCTAGG C^CTAGG
BseAI 2822 TCCGGA T^CCGGA
BsiEI 1506 CGACCG CG[GA][CT]^CG
BspEI 2822 TCCGGA T^CCGGA
BssHII 1052 GCGCGC G^CGCGC
BstAPI 1021 GCAGGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3026 GGTGACC G^GT[ACGT]ACC
BstPI 3026 GGTGACC G^GT[ACGT]ACC
DrdI 965 GACAAGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 3356 GACCCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 78 AGCGCT AGC^GCT
EcoO65I 3026 GGTGACC G^GT[ACGT]ACC
EcoRI 3134 GAATTC G^AATTC
EcoT22I 1210 ATGCAT ATGCA^T
KasI 442 GGCGCC G^GCGCC
KpnI 2520 GGTACC GGTAC^C
MfeI 5005 CAATTG C^AATTG
MroI 2822 TCCGGA T^CCGGA
MunI 5005 CAATTG C^AATTG
NarI 442 GGCGCC GG^CGCC
NcoI 945 CCATGG C^CATGG
NsiI 1210 ATGCAT ATGCA^T
PaeR7I 573 CTCGAG C^TCGAG
PshAI 2734 GACGCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 442 GGCGCC GGC^GCC
SmaI 3036 CCCGGG CCC^GGG
StuI 2220 AGGCCT AGG^CCT
XbaI 1864 TCTAGA T^CTAGA
XhoI 573 CTCGAG C^TCGAG
XmaCI 3036 CCCGGG C^CCGGG
XmaI 3036 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 776 AAATTT [GA]^AATT[CT]
3134 GAATTC
AocI 182 CCTAAGG CC^T[ACGT]AGG
734 CCTGAGG
ApoI 776 AAATTT [GA]^AATT[CT]
3134 GAATTC
Asp700I 23 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1087 GAACCCCTTC
AviII 1311 TGCGCA TGC^GCA
4603 TGCGCA
BbsI 3632 GAAGAC GAAGAC(2/6)
57 GTCTTC
BbvCI 481 CCTCAGC CCTCAGC(-5/-2)
421 GCTGAGG
BlpI 337 GCTGAGC GC^T[ACGT]AGC
4546 GCTGAGC
Bpu1102I 337 GCTGAGC GC^T[ACGT]AGC
4546 GCTGAGC
BpuAI 3632 GAAGAC GAAGAC(2/6)
57 GTCTTC
BsaWI 1746 ACCGGA [AT]^CCGG[AT]
2822 TCCGGA
BsmI 1208 GAATGC GAATGC(1/-1)
2617 GAATGC
Bsu36I 182 CCTAAGG CC^T[ACGT]AGG
734 CCTGAGG
CelII 337 GCTGAGC GC^T[ACGT]AGC
4546 GCTGAGC
DraIII 560 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
2701 CACAGTGTG
Eco81I 182 CCTAAGG CC^T[ACGT]AGG
734 CCTGAGG
FspI 1311 TGCGCA TGC^GCA
4603 TGCGCA
HaeII 78 AGCGCT [GA]GCGC^[CT]
442 GGCGCC
NdeI 2445 CATATG CA^TATG
4493 CATATG
NspI 1026 ACATGC [GA]CATG^[CT]
4426 ACATGC
PvuII 1042 CAGCTG CAG^CTG
2027 CAGCTG
SacI 2267 GAGCTC GAGCT^C
3853 GAGCTC
SbfI 808 CCTGCAGG CCTGCA^GG
3688 CCTGCAGG
SexAI 1998 ACCAGGT A^CC[AT]GGT
4891 ACCTGGT
SmlI 573 CTCGAG C^T[CT][GA]AG
1100 CTCAAG
Sse8387I 808 CCTGCAGG CCTGCA^GG
3688 CCTGCAGG
XmnI 23 GAATGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1087 GAACCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AflIII AluI AlwI AlwNI AspHI
AspI AvaI AvaII BalI BanI
BanII BbrPI BbvI BciVI BcnI
BfaI BmyI BpmI BsaAI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PflFI PflMI PleI PmaCI PmlI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII