################################## # NO CUT ENZYMES ( 61 enzymes) # ################################## AccI AclI AflII Aor51HI AscI AseI AsnI AviII AvrII BbrPI BcgI BfrI BglII BlnI Bsp1407I BspDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DrdI Eco47III EcoNI EcoO65I FseI FspI HincII HindII HpaI MfeI MluI MunI NdeI NheI NotI NruI NspV PacI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI SexAI SfiI SfuI SnaBI SpeI SphI SspBI SwaI XbaI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AatII 34 GACGTC GACGT^C Acc65I 3065 GGTACC G^GTACC AccIII 3092 TCCGGA T^CCGGA AgeI 2328 ACCGGT A^CCGGT Alw44I 390 GTGCAC G^TGCAC ApaLI 390 GTGCAC G^TGCAC Asp718I 3065 GGTACC G^GTACC BamHI 4595 GGATCC G^GATCC BclI 2601 TGATCA T^GATCA BsaBI 1128 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 3092 TCCGGA T^CCGGA BsiWI 4511 CGTACG C^GTACG BspEI 3092 TCCGGA T^CCGGA DraI 4781 TTTAAA TTT^AAA DraIII 59 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoRV 1987 GATATC GAT^ATC EcoT22I 1129 ATGCAT ATGCA^T FbaI 2601 TGATCA T^GATCA KpnI 3065 GGTACC GGTAC^C MamI 1128 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 3092 TCCGGA T^CCGGA NsiI 1129 ATGCAT ATGCA^T PinAI 2328 ACCGGT A^CCGGT PshAI 2185 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SbfI 4429 CCTGCAGG CCTGCA^GG ScaI 3121 AGTACT AGT^ACT Sse8387I 4429 CCTGCAGG CCTGCA^GG SspI 8 AATATT AAT^ATT XcmI 2342 CCATCAACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AhdI 2446 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4077 GACAGCTTGTC ApaI 186 GGGCCC GGGCC^C 2790 GGGCCC Asp700I 2266 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2916 GAATAACTTC AspEI 2446 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4077 GACAGCTTGTC BalI 1868 TGGCCA TGG^CCA 2354 TGGCCA BbeI 502 GGCGCC GGCGC^C 643 GGCGCC BbsI 2386 GAAGAC GAAGAC(2/6) 2893 GTCTTC BciVI 4032 GGATAC GTATCC(6/5) 706 GTATCC BglI 3488 GCCGGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4652 GCCAGCAAGGC BlpI 3507 GCTGAGC GC^T[ACGT]AGC 4056 GCTCAGC Bpu1102I 3507 GCTGAGC GC^T[ACGT]AGC 4056 GCTCAGC BpuAI 2386 GAAGAC GAAGAC(2/6) 2893 GTCTTC BsaAI 2424 CACGTA [CT]AC^GT[GA] 4509 CACGTA BsgI 2124 GTGCAG GTGCAG(16/14) 3654 GTGCAG BsmI 3597 GAATGC GAATGC(1/-1) 4943 GCATTC BspLU11I 2874 ACATGT A^CATGT 4474 ACATGT BstAPI 1201 GCACTGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2615 GCATCGACTGC BstYI 3161 AGATCC [GA]^GATC[CT] 4595 GGATCC CelII 3507 GCTGAGC GC^T[ACGT]AGC 4056 GCTCAGC Eam1105I 2446 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4077 GACAGCTTGTC EcoRI 312 GAATTC G^AATTC 3874 GAATTC HindIII 1323 AAGCTT A^AGCTT 2313 AAGCTT KasI 502 GGCGCC G^GCGCC 643 GGCGCC MflI 3161 AGATCC [GA]^GATC[CT] 4595 GGATCC MluNI 1868 TGGCCA TGG^CCA 2354 TGGCCA MscI 1868 TGGCCA TGG^CCA 2354 TGGCCA NarI 502 GGCGCC GG^CGCC 643 GGCGCC PaeR7I 1691 CTCGAG C^TCGAG 4037 CTCGAG PpuMI 1305 GGGTCCT [GA]G^G[AT]CC[CT] 2202 AGGACCT SfoI 502 GGCGCC GGC^GCC 643 GGCGCC StuI 2289 AGGCCT AGG^CCT 3587 AGGCCT XhoI 1691 CTCGAG C^TCGAG 4037 CTCGAG XhoII 3161 AGATCC [GA]^GATC[CT] 4595 GGATCC XmnI 2266 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2916 GAATAACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 134 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AspI AvaI AvaII BanI BanII BbvCI BbvI BcnI BfaI BmyI BpmI BsaHI BsaI BsaJI BsaWI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstUI BstXI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PflFI PflMI PleI PstI PvuII RcaI RsaI SacI SacII SapI Sau3AI Sau96I ScrFI SfaNI SfcI SgrAI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XmaCI XmaI