##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AccI AclI AflII Aor51HI AscI
AseI AsnI AviII AvrII BbrPI
BcgI BfrI BglII BlnI Bsp1407I
BspDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DrdI Eco47III EcoNI EcoO65I
FseI FspI HincII HindII HpaI
MfeI MluI MunI NdeI NheI
NotI NruI NspV PacI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsrII SalI SexAI SfiI SfuI
SnaBI SpeI SphI SspBI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AatII 34 GACGTC GACGT^C
Acc65I 3065 GGTACC G^GTACC
AccIII 3092 TCCGGA T^CCGGA
AgeI 2328 ACCGGT A^CCGGT
Alw44I 390 GTGCAC G^TGCAC
ApaLI 390 GTGCAC G^TGCAC
Asp718I 3065 GGTACC G^GTACC
BamHI 4595 GGATCC G^GATCC
BclI 2601 TGATCA T^GATCA
BsaBI 1128 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3092 TCCGGA T^CCGGA
BsiWI 4511 CGTACG C^GTACG
BspEI 3092 TCCGGA T^CCGGA
DraI 4781 TTTAAA TTT^AAA
DraIII 59 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoRV 1987 GATATC GAT^ATC
EcoT22I 1129 ATGCAT ATGCA^T
FbaI 2601 TGATCA T^GATCA
KpnI 3065 GGTACC GGTAC^C
MamI 1128 GATGCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3092 TCCGGA T^CCGGA
NsiI 1129 ATGCAT ATGCA^T
PinAI 2328 ACCGGT A^CCGGT
PshAI 2185 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SbfI 4429 CCTGCAGG CCTGCA^GG
ScaI 3121 AGTACT AGT^ACT
Sse8387I 4429 CCTGCAGG CCTGCA^GG
SspI 8 AATATT AAT^ATT
XcmI 2342 CCATCAACCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AhdI 2446 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4077 GACAGCTTGTC
ApaI 186 GGGCCC GGGCC^C
2790 GGGCCC
Asp700I 2266 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2916 GAATAACTTC
AspEI 2446 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4077 GACAGCTTGTC
BalI 1868 TGGCCA TGG^CCA
2354 TGGCCA
BbeI 502 GGCGCC GGCGC^C
643 GGCGCC
BbsI 2386 GAAGAC GAAGAC(2/6)
2893 GTCTTC
BciVI 4032 GGATAC GTATCC(6/5)
706 GTATCC
BglI 3488 GCCGGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4652 GCCAGCAAGGC
BlpI 3507 GCTGAGC GC^T[ACGT]AGC
4056 GCTCAGC
Bpu1102I 3507 GCTGAGC GC^T[ACGT]AGC
4056 GCTCAGC
BpuAI 2386 GAAGAC GAAGAC(2/6)
2893 GTCTTC
BsaAI 2424 CACGTA [CT]AC^GT[GA]
4509 CACGTA
BsgI 2124 GTGCAG GTGCAG(16/14)
3654 GTGCAG
BsmI 3597 GAATGC GAATGC(1/-1)
4943 GCATTC
BspLU11I 2874 ACATGT A^CATGT
4474 ACATGT
BstAPI 1201 GCACTGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2615 GCATCGACTGC
BstYI 3161 AGATCC [GA]^GATC[CT]
4595 GGATCC
CelII 3507 GCTGAGC GC^T[ACGT]AGC
4056 GCTCAGC
Eam1105I 2446 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4077 GACAGCTTGTC
EcoRI 312 GAATTC G^AATTC
3874 GAATTC
HindIII 1323 AAGCTT A^AGCTT
2313 AAGCTT
KasI 502 GGCGCC G^GCGCC
643 GGCGCC
MflI 3161 AGATCC [GA]^GATC[CT]
4595 GGATCC
MluNI 1868 TGGCCA TGG^CCA
2354 TGGCCA
MscI 1868 TGGCCA TGG^CCA
2354 TGGCCA
NarI 502 GGCGCC GG^CGCC
643 GGCGCC
PaeR7I 1691 CTCGAG C^TCGAG
4037 CTCGAG
PpuMI 1305 GGGTCCT [GA]G^G[AT]CC[CT]
2202 AGGACCT
SfoI 502 GGCGCC GGC^GCC
643 GGCGCC
StuI 2289 AGGCCT AGG^CCT
3587 AGGCCT
XhoI 1691 CTCGAG C^TCGAG
4037 CTCGAG
XhoII 3161 AGATCC [GA]^GATC[CT]
4595 GGATCC
XmnI 2266 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2916 GAATAACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AspI AvaI AvaII
BanI BanII BbvCI BbvI BcnI
BfaI BmyI BpmI BsaHI BsaI
BsaJI BsaWI BseRI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstXI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EagI EarI
EclXI Eco52I Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PflFI PflMI
PleI PstI PvuII RcaI RsaI
SacI SacII SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SgrAI SmaI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XmaCI XmaI