##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AgeI AscI Asp718I AvaI BbeI
BcgI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrGI BssHII
BssSI Bst1107I BstZ17I ClaI CpoI
EagI EclXI Eco52I FseI Hin1I
KasI KpnI KspI MamI MfeI
MluI MroI MunI NarI NotI
NruI PacI PaeR7I PinAI PmeI
PvuI RsrII SacII SalI SapI
ScaI SfiI SfoI SgrAI SmaI
SnaBI SpeI SspBI SwaI XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccII 2871 CGCG CG^CG
AflII 508 CTTAAG C^TTAAG
AflIII 4987 ACACGT A^C[GA][CT]GT
AhdI 969 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4229 GTGCAC G^TGCAC
Aor51HI 1406 AGCGCT AGC^GCT
ApaLI 4229 GTGCAC G^TGCAC
AseI 2458 ATTAAT AT^TAAT
AsnI 2458 ATTAAT AT^TAAT
AspEI 969 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 635 TGCGCA TGC^GCA
BamHI 275 GGATCC G^GATCC
BbrPI 820 CACGTG CAC^GTG
BciVI 1739 GGATAC GTATCC(6/5)
BfrI 508 CTTAAG C^TTAAG
BglI 2723 GCCACAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1166 AGATCT A^GATCT
BsaAI 820 CACGTG [CT]AC^GT[GA]
BspHI 1284 TCATGA T^CATGA
BsrBI 609 CCGCTC CCGCTC(-3/-3)
BsrFI 3702 GCCGGC [GA]^CCGG[CT]
BstAPI 816 GCATCACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2299 TTCGAA TT^CGAA
BstUI 2871 CGCG CG^CG
BstXI 4234 CCAAGACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 3702 GCCGGC [GA]^CCGG[CT]
DrdI 4153 GACCTGGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 969 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1406 AGCGCT AGC^GCT
EcoRI 746 GAATTC G^AATTC
EcoRV 3908 GATATC GAT^ATC
EcoT22I 5330 ATGCAT ATGCA^T
FspI 635 TGCGCA TGC^GCA
HaeII 1406 AGCGCT [GA]GCGC^[CT]
HgaI 1912 GACGC GACGC(5/10)
HindIII 4054 AAGCTT A^AGCTT
HpaI 1482 GTTAAC GTT^AAC
MvnI 2871 CGCG CG^CG
NaeI 3702 GCCGGC GCC^GGC
NdeI 1805 CATATG CA^TATG
NgoMIV 3702 GCCGGC G^CCGGC
NsiI 5330 ATGCAT ATGCA^T
NspV 2299 TTCGAA TT^CGAA
PmaCI 820 CACGTG CAC^GTG
PmlI 820 CACGTG CAC^GTG
PshAI 4238 GACTATGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2458 ATTAAT AT^TAAT
RcaI 1284 TCATGA T^CATGA
SbfI 1985 CCTGCAGG CCTGCA^GG
SexAI 2045 ACCTGGT A^CC[AT]GGT
SfuI 2299 TTCGAA TT^CGAA
Sse8387I 1985 CCTGCAGG CCTGCA^GG
SspI 2455 AATATT AAT^ATT
StuI 1413 AGGCCT AGG^CCT
XcmI 603 CCAGTGCCGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AclI 1634 AACGTT AA^CGTT
2656 AACGTT
AocI 810 CCTCAGG CC^T[ACGT]AGG
1010 CCTGAGG
AvrII 3751 CCTAGG C^CTAGG
3977 CCTAGG
BanI 1998 GGTGCC G^G[CT][GA]CC
3227 GGCACC
BlnI 3751 CCTAGG C^CTAGG
3977 CCTAGG
BstEII 718 GGTGACC G^GT[ACGT]ACC
1187 GGTTACC
BstPI 718 GGTGACC G^GT[ACGT]ACC
1187 GGTTACC
Bsu36I 810 CCTCAGG CC^T[ACGT]AGG
1010 CCTGAGG
DraIII 3249 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
3730 CACAGGGTG
Eco81I 810 CCTCAGG CC^T[ACGT]AGG
1010 CCTGAGG
EcoNI 1928 CCTGGCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5037 CCTGACTAAGG
EcoO65I 718 GGTGACC G^GT[ACGT]ACC
1187 GGTTACC
NheI 2122 GCTAGC G^CTAGC
3213 GCTAGC
Psp1406I 1634 AACGTT AA^CGTT
2656 AACGTT
SphI 3342 GCATGC GCATG^C
4017 GCATGC
TfiI 1593 GATTC G^A[AT]TC
4584 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI Asp700I AspHI
AspI AvaII BalI BanII BbsI
BbvCI BbvI BclI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspMI BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I Van91I XhoII XmnI