##################################
# NO CUT ENZYMES ( 63 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AspI BbrPI BclI BfrI BsaI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BspHI Bst1107I BstBI BstZ17I
ClaI CpoI EagI EclXI Eco47III
Eco52I FbaI FseI HpaI MfeI
MluI MroI MunI NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SfiI
SfuI SgrAI SnaBI SphI Sse8387I
SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 3118 GGTACC G^GTACC
AccI 249 GTCTAC GT^[AC][GT]AC
AflIII 1465 ACATGT A^C[GA][CT]GT
AhdI 3627 GACCCCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 3889 ATTAAT AT^TAAT
AsnI 3889 ATTAAT AT^TAAT
Asp718I 3118 GGTACC G^GTACC
AspEI 3627 GACCCCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2119 TGCGCA TGC^GCA
AvrII 1163 CCTAGG C^CTAGG
BalI 3613 TGGCCA TGG^CCA
BbeI 45 GGCGCC GGCGC^C
BcgI 806 CGATACGACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 4642 AGATCT A^GATCT
BlnI 1163 CCTAGG C^CTAGG
BlpI 4157 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 4157 GCTGAGC GC^T[ACGT]AGC
BsaAI 107 TACGTG [CT]AC^GT[GA]
BsmBI 3289 GAGACG CGTCTC(1/5)
BspLU11I 1465 ACATGT A^CATGT
BstAPI 890 GCAGTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3279 GGTGACC G^GT[ACGT]ACC
BstPI 3279 GGTGACC G^GT[ACGT]ACC
BstXI 3645 CCAAGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 4157 GCTGAGC GC^T[ACGT]AGC
Eam1105I 3627 GACCCCTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3279 GGTGACC G^GT[ACGT]ACC
EcoRI 86 GAATTC G^AATTC
EcoRV 1661 GATATC GAT^ATC
FspI 2119 TGCGCA TGC^GCA
KasI 45 GGCGCC G^GCGCC
KpnI 3118 GGTACC GGTAC^C
MluNI 3613 TGGCCA TGG^CCA
MscI 3613 TGGCCA TGG^CCA
NaeI 208 GCCGGC GCC^GGC
NarI 45 GGCGCC GG^CGCC
NcoI 1904 CCATGG C^CATGG
NdeI 4785 CATATG CA^TATG
NgoMIV 208 GCCGGC G^CCGGC
NheI 3383 GCTAGC G^CTAGC
PaeR7I 3391 CTCGAG C^TCGAG
PshAI 1748 GACGAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3889 ATTAAT AT^TAAT
SacI 3604 GAGCTC GAGCT^C
SexAI 4300 ACCTGGT A^CC[AT]GGT
SfoI 45 GGCGCC GGC^GCC
SpeI 3108 ACTAGT A^CTAGT
StuI 3477 AGGCCT AGG^CCT
XhoI 3391 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AocI 162 CCTGAGG CC^T[ACGT]AGG
3507 CCTTAGG
Asp700I 433 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2377 GAATGGGTTC
BamHI 492 GGATCC G^GATCC
1204 GGATCC
BciVI 2714 GGATAC GTATCC(6/5)
3655 GGATAC
BglI 309 GCCTGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3296 GCCCTCCTGGC
BsrDI 2999 CATTGC GCAATG(2/0)
4791 GCAATG
BssSI 661 CACGAG CACGAG(-5/-1)
1719 CACGAG
Bsu36I 162 CCTGAGG CC^T[ACGT]AGG
3507 CCTTAGG
DraI 2599 TTTAAA TTT^AAA
4813 TTTAAA
DrdI 159 GACCCTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
192 GACGGGGTGGTC
Eco81I 162 CCTGAGG CC^T[ACGT]AGG
3507 CCTTAGG
EcoT22I 1651 ATGCAT ATGCA^T
2238 ATGCAT
HincII 2899 GTTGAC GT[CT]^[GA]AC
3745 GTTGAC
HindII 2899 GTTGAC GT[CT]^[GA]AC
3745 GTTGAC
KspI 11 CCGCGG CCGC^GG
261 CCGCGG
NsiI 1651 ATGCAT ATGCA^T
2238 ATGCAT
NspI 1465 ACATGT [GA]CATG^[CT]
3265 GCATGT
SacII 11 CCGCGG CCGC^GG
261 CCGCGG
SapI 785 GAAGAGC GCTCTTC(1/4)
1526 GCTCTTC
SmaI 17 CCCGGG CCC^GGG
231 CCCGGG
XbaI 974 TCTAGA T^CTAGA
2276 TCTAGA
XmaCI 17 CCCGGG C^CCGGG
231 CCCGGG
XmaI 17 CCCGGG C^CCGGG
231 CCCGGG
XmnI 433 GAAGTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2377 GAATGGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaBI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspMI BsrBI BsrFI BsrGI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII