##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI AscI AspEI AspI BbeI
BcgI BsaHI BseAI BsiWI BsmBI
BspEI BstBI BstEII BstPI BstXI
CpoI DrdI EagI Eam1105I EclXI
Eco52I EcoNI EcoO65I EcoRV FseI
HgaI Hin1I KasI MluI MroI
NaeI NarI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI Psp1406I RsrII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI Sse8387I SspI
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 515 GGTACC G^GTACC
AccI 2972 GTATAC GT^[AC][GT]AC
Alw44I 2837 GTGCAC G^TGCAC
Aor51HI 690 AGCGCT AGC^GCT
ApaLI 2837 GTGCAC G^TGCAC
AseI 3004 ATTAAT AT^TAAT
AsnI 3004 ATTAAT AT^TAAT
Asp718I 515 GGTACC G^GTACC
AvaI 526 CTCGGG C^[CT]CG[GA]G
AviII 5050 TGCGCA TGC^GCA
BamHI 5812 GGATCC G^GATCC
BanI 515 GGTACC G^G[CT][GA]CC
BbrPI 341 CACGTG CAC^GTG
BclI 291 TGATCA T^GATCA
BglI 5786 GCCACCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1801 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1801 GCTGAGC GC^T[ACGT]AGC
BsaI 1640 GGTCTC GGTCTC(1/5)
BsoBI 526 CTCGGG C^[CT]CG[GA]G
Bsp1407I 4594 TGTACA T^GTACA
BsrBI 131 GAGCGG CCGCTC(-3/-3)
BsrGI 4594 TGTACA T^GTACA
BssHII 8 GCGCGC G^CGCGC
BssSI 2500 CACGAG CACGAG(-5/-1)
Bst1107I 2972 GTATAC GTA^TAC
BstZ17I 2972 GTATAC GTA^TAC
CelII 1801 GCTGAGC GC^T[ACGT]AGC
Eco47III 690 AGCGCT AGC^GCT
FbaI 291 TGATCA T^GATCA
FspI 5050 TGCGCA TGC^GCA
HindIII 991 AAGCTT A^AGCTT
HpaI 5039 GTTAAC GTT^AAC
KpnI 515 GGTACC GGTAC^C
NcoI 2596 CCATGG C^CATGG
NheI 5338 GCTAGC G^CTAGC
PmaCI 341 CACGTG CAC^GTG
PmlI 341 CACGTG CAC^GTG
PshBI 3004 ATTAAT AT^TAAT
PvuI 48 CGATCG CGAT^CG
SacI 4732 GAGCTC GAGCT^C
SexAI 2424 ACCTGGT A^CC[AT]GGT
SphI 4559 GCATGC GCATG^C
SspBI 4594 TGTACA T^GTACA
XbaI 1210 TCTAGA T^CTAGA
XcmI 2211 CCACAGGCAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
ApaI 751 GGGCCC GGGCC^C
2385 GGGCCC
BalI 801 TGGCCA TGG^CCA
1971 TGGCCA
BbvCI 4667 CCTCAGC CCTCAGC(-5/-2)
5207 CCTCAGC
BglII 2507 AGATCT A^GATCT
3812 AGATCT
BsaAI 341 CACGTG [CT]AC^GT[GA]
407 TACGTG
BsaBI 310 GATCCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5138 GATTCCTATC
BsaWI 705 ACCGGA [AT]^CCGG[AT]
1644 TCCGGT
BsmI 5888 GAATGC GAATGC(1/-1)
5212 GCATTC
BspDI 1672 ATCGAT AT^CGAT
2404 ATCGAT
BsrFI 478 GCCGGT [GA]^CCGG[CT]
4011 GCCGGT
Cfr10I 478 GCCGGT [GA]^CCGG[CT]
4011 GCCGGT
ClaI 1672 ATCGAT AT^CGAT
2404 ATCGAT
EarI 3599 CTCTTC CTCTTC(1/4)
2013 GAAGAG
EcoRI 4216 GAATTC G^AATTC
5321 GAATTC
Ksp632I 3599 CTCTTC CTCTTC(1/4)
2013 GAAGAG
KspI 100 CCGCGG CCGC^GG
180 CCGCGG
MamI 310 GATCCCGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5138 GATTCCTATC
MfeI 2444 CAATTG C^AATTG
3410 CAATTG
MluNI 801 TGGCCA TGG^CCA
1971 TGGCCA
MscI 801 TGGCCA TGG^CCA
1971 TGGCCA
MunI 2444 CAATTG C^AATTG
3410 CAATTG
PshAI 586 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2608 GACCTGAGTC
SacII 100 CCGCGG CCGC^GG
180 CCGCGG
ScaI 1997 AGTACT AGT^ACT
3560 AGTACT
SpeI 1249 ACTAGT A^CTAGT
2531 ACTAGT
TaqI 1673 TCGA T^CGA
2405 TCGA
TthHB8I 1673 TCGA T^CGA
2405 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AflIII AluI AlwI AlwNI AocI
ApoI Asp700I AspHI AvaII AvrII
BanII BbsI BbvI BciVI BcnI
BfaI BfrI BlnI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspHI BspLU11I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI Eco81I EcoO109I
EcoRII EcoT14I EcoT22I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII
XmnI