##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AccI AclI AflII AgeI AhdI
AscI AspEI AvrII BamHI BbeI
BbrPI BfrI BglI BlnI BlpI
Bpu1102I BsaAI BsiEI BsiWI Bsp1407I
BspDI BsrBI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I CelII
ClaI CpoI EagI Eam1105I EclXI
Eco52I EcoO65I FseI HpaI KasI
KspI MfeI MluI MunI NarI
NotI NruI NspV PacI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SmaI SnaBI Sse8387I
SspBI SwaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 1941 GACGTC GACGT^C
AccII 546 CGCG CG^CG
AccIII 2643 TCCGGA T^CCGGA
Alw44I 4325 GTGCAC G^TGCAC
Aor51HI 2511 AGCGCT AGC^GCT
ApaI 1669 GGGCCC GGGCC^C
ApaLI 4325 GTGCAC G^TGCAC
AseI 2343 ATTAAT AT^TAAT
AsnI 2343 ATTAAT AT^TAAT
Asp700I 1622 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 4100 CTCGAG C^[CT]CG[GA]G
BbvCI 1499 CCTCAGC CCTCAGC(-5/-2)
BcgI 1726 CGAAAGTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 4893 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2643 TCCGGA T^CCGGA
BsoBI 4100 CTCGAG C^[CT]CG[GA]G
BspEI 2643 TCCGGA T^CCGGA
BstUI 546 CGCG CG^CG
DraIII 2535 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 2511 AGCGCT AGC^GCT
EcoRV 3224 GATATC GAT^ATC
HincII 3882 GTTGAC GT[CT]^[GA]AC
HindII 3882 GTTGAC GT[CT]^[GA]AC
MamI 4893 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2643 TCCGGA T^CCGGA
MvnI 546 CGCG CG^CG
NaeI 3000 GCCGGC GCC^GGC
NgoMIV 3000 GCCGGC G^CCGGC
NheI 54 GCTAGC G^CTAGC
PaeR7I 4100 CTCGAG C^TCGAG
PshAI 4803 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2343 ATTAAT AT^TAAT
ScaI 3555 AGTACT AGT^ACT
SexAI 1447 ACCTGGT A^CC[AT]GGT
SgrAI 1743 CACCGGCG C[GA]^CCGG[CT]G
SpeI 3808 ACTAGT A^CTAGT
SphI 4047 GCATGC GCATG^C
StuI 1535 AGGCCT AGG^CCT
XhoI 4100 CTCGAG C^TCGAG
XmnI 1622 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 2703 GGTACC G^GTACC
2883 GGTACC
AcyI 1941 GACGTC G[GA]^CG[CT]C
4527 GGCGTC
Asp718I 2703 GGTACC G^GTACC
2883 GGTACC
AspI 1446 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1485 GACTCTGTC
AviII 1822 TGCGCA TGC^GCA
4895 TGCGCA
BclI 998 TGATCA T^GATCA
4200 TGATCA
BcnI 1799 CCGGG CC^[GC]GG
2652 CCCGG
BsaHI 1941 GACGTC G[GA]^CG[CT]C
4527 GGCGTC
BsaI 3123 GAGACC GGTCTC(1/5)
4695 GGTCTC
BsaWI 2643 TCCGGA [AT]^CCGG[AT]
2880 TCCGGT
BsgI 3151 GTGCAG GTGCAG(16/14)
4214 GTGCAG
BsmBI 4529 CGTCTC CGTCTC(1/5)
3389 GAGACG
BspHI 1997 TCATGA T^CATGA
5197 TCATGA
BspMI 691 ACCTGC ACCTGC(4/8)
4036 ACCTGC
BssSI 423 CACGAG CACGAG(-5/-1)
5039 CTCGTG
BstAPI 1713 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5739 GCAGAAACTGC
DrdI 2829 GACAGGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5598 GACAGACATGTC
EcoRI 296 GAATTC G^AATTC
887 GAATTC
EcoT22I 2727 ATGCAT ATGCA^T
4049 ATGCAT
FbaI 998 TGATCA T^GATCA
4200 TGATCA
FspI 1822 TGCGCA TGC^GCA
4895 TGCGCA
HgaI 476 GACGC GACGC(5/10)
4528 GCGTC
Hin1I 1941 GACGTC G[GA]^CG[CT]C
4527 GGCGTC
KpnI 2703 GGTACC GGTAC^C
2883 GGTACC
NciI 1799 CCGGG CC^[GC]GG
2652 CCCGG
NdeI 4422 CATATG CA^TATG
5239 CATATG
NsiI 2727 ATGCAT ATGCA^T
4049 ATGCAT
PflFI 1446 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1485 GACTCTGTC
RcaI 1997 TCATGA T^CATGA
5197 TCATGA
SapI 224 GAAGAGC GCTCTTC(1/4)
2604 GAAGAGC
Tth111I 1446 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1485 GACTCTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI AspHI
AvaII BalI BanI BanII BbsI
BbvI BciVI BfaI BglII BmyI
BpmI BpuAI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BspLU11I BsrDI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XcmI XhoII