################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## AccI AclI AflII AgeI AhdI AscI AspEI AvrII BamHI BbeI BbrPI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsiEI BsiWI Bsp1407I BspDI BsrBI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI EagI Eam1105I EclXI Eco52I EcoO65I FseI HpaI KasI KspI MfeI MluI MunI NarI NotI NruI NspV PacI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SmaI SnaBI Sse8387I SspBI SwaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AatII 1941 GACGTC GACGT^C AccII 546 CGCG CG^CG AccIII 2643 TCCGGA T^CCGGA Alw44I 4325 GTGCAC G^TGCAC Aor51HI 2511 AGCGCT AGC^GCT ApaI 1669 GGGCCC GGGCC^C ApaLI 4325 GTGCAC G^TGCAC AseI 2343 ATTAAT AT^TAAT AsnI 2343 ATTAAT AT^TAAT Asp700I 1622 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 4100 CTCGAG C^[CT]CG[GA]G BbvCI 1499 CCTCAGC CCTCAGC(-5/-2) BcgI 1726 CGAAAGTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaBI 4893 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 2643 TCCGGA T^CCGGA BsoBI 4100 CTCGAG C^[CT]CG[GA]G BspEI 2643 TCCGGA T^CCGGA BstUI 546 CGCG CG^CG DraIII 2535 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 2511 AGCGCT AGC^GCT EcoRV 3224 GATATC GAT^ATC HincII 3882 GTTGAC GT[CT]^[GA]AC HindII 3882 GTTGAC GT[CT]^[GA]AC MamI 4893 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2643 TCCGGA T^CCGGA MvnI 546 CGCG CG^CG NaeI 3000 GCCGGC GCC^GGC NgoMIV 3000 GCCGGC G^CCGGC NheI 54 GCTAGC G^CTAGC PaeR7I 4100 CTCGAG C^TCGAG PshAI 4803 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2343 ATTAAT AT^TAAT ScaI 3555 AGTACT AGT^ACT SexAI 1447 ACCTGGT A^CC[AT]GGT SgrAI 1743 CACCGGCG C[GA]^CCGG[CT]G SpeI 3808 ACTAGT A^CTAGT SphI 4047 GCATGC GCATG^C StuI 1535 AGGCCT AGG^CCT XhoI 4100 CTCGAG C^TCGAG XmnI 1622 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### Acc65I 2703 GGTACC G^GTACC 2883 GGTACC AcyI 1941 GACGTC G[GA]^CG[CT]C 4527 GGCGTC Asp718I 2703 GGTACC G^GTACC 2883 GGTACC AspI 1446 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1485 GACTCTGTC AviII 1822 TGCGCA TGC^GCA 4895 TGCGCA BclI 998 TGATCA T^GATCA 4200 TGATCA BcnI 1799 CCGGG CC^[GC]GG 2652 CCCGG BsaHI 1941 GACGTC G[GA]^CG[CT]C 4527 GGCGTC BsaI 3123 GAGACC GGTCTC(1/5) 4695 GGTCTC BsaWI 2643 TCCGGA [AT]^CCGG[AT] 2880 TCCGGT BsgI 3151 GTGCAG GTGCAG(16/14) 4214 GTGCAG BsmBI 4529 CGTCTC CGTCTC(1/5) 3389 GAGACG BspHI 1997 TCATGA T^CATGA 5197 TCATGA BspMI 691 ACCTGC ACCTGC(4/8) 4036 ACCTGC BssSI 423 CACGAG CACGAG(-5/-1) 5039 CTCGTG BstAPI 1713 GCAGCAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 5739 GCAGAAACTGC DrdI 2829 GACAGGAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 5598 GACAGACATGTC EcoRI 296 GAATTC G^AATTC 887 GAATTC EcoT22I 2727 ATGCAT ATGCA^T 4049 ATGCAT FbaI 998 TGATCA T^GATCA 4200 TGATCA FspI 1822 TGCGCA TGC^GCA 4895 TGCGCA HgaI 476 GACGC GACGC(5/10) 4528 GCGTC Hin1I 1941 GACGTC G[GA]^CG[CT]C 4527 GGCGTC KpnI 2703 GGTACC GGTAC^C 2883 GGTACC NciI 1799 CCGGG CC^[GC]GG 2652 CCCGG NdeI 4422 CATATG CA^TATG 5239 CATATG NsiI 2727 ATGCAT ATGCA^T 4049 ATGCAT PflFI 1446 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1485 GACTCTGTC RcaI 1997 TCATGA T^CATGA 5197 TCATGA SapI 224 GAAGAGC GCTCTTC(1/4) 2604 GAAGAGC Tth111I 1446 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1485 GACTCTGTC ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AvaII BalI BanI BanII BbsI BbvI BciVI BfaI BglII BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspLU11I BsrDI BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XbaI XcmI XhoII