##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
Acc65I AccI AclI AgeI AscI
AseI AsnI Asp700I Asp718I AvrII
BbrPI BcgI BclI BlnI BsaBI
BsiWI BsmI BspDI BspHI Bst1107I
BstZ17I ClaI DrdI EcoT22I FbaI
HincII HindII HindIII HpaI KpnI
MamI MfeI MunI NdeI NotI
NruI NsiI PacI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI SalI SexAI SfiI
SpeI SwaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccIII 226 TCCGGA T^CCGGA
AcsI 3214 GAATTC [GA]^AATT[CT]
AflII 63 CTTAAG C^TTAAG
AhdI 4342 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 429 GTGCAC G^TGCAC
AocI 2295 CCTAAGG CC^T[ACGT]AGG
ApaLI 429 GTGCAC G^TGCAC
ApoI 3214 GAATTC [GA]^AATT[CT]
AspEI 4342 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2496 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1408 TGCGCA TGC^GCA
BalI 1391 TGGCCA TGG^CCA
BbvCI 1222 CCTCAGC CCTCAGC(-5/-2)
BfrI 63 CTTAAG C^TTAAG
BglII 163 AGATCT A^GATCT
BsaAI 5053 TACGTA [CT]AC^GT[GA]
BseAI 226 TCCGGA T^CCGGA
BspEI 226 TCCGGA T^CCGGA
BstAPI 4306 GCATGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3786 TTCGAA TT^CGAA
Bsu36I 2295 CCTAAGG CC^T[ACGT]AGG
CpoI 3127 CGGTCCG CG^G[AT]CCG
DraIII 3917 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 4342 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2295 CCTAAGG CC^T[ACGT]AGG
EcoRI 3214 GAATTC G^AATTC
EcoRV 138 GATATC GAT^ATC
FseI 399 GGCCGGCC GGCCGG^CC
FspI 1408 TGCGCA TGC^GCA
MluI 244 ACGCGT A^CGCGT
MluNI 1391 TGGCCA TGG^CCA
MroI 226 TCCGGA T^CCGGA
MscI 1391 TGGCCA TGG^CCA
NspV 3786 TTCGAA TT^CGAA
PflFI 2496 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1230 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsrII 3127 CGGTCCG CG^G[AT]CCG
SapI 4377 GAAGAGC GCTCTTC(1/4)
SbfI 1308 CCTGCAGG CCTGCA^GG
ScaI 4389 AGTACT AGT^ACT
SfuI 3786 TTCGAA TT^CGAA
SmaI 656 CCCGGG CCC^GGG
SnaBI 5053 TACGTA TAC^GTA
Sse8387I 1308 CCTGCAGG CCTGCA^GG
SspI 5074 AATATT AAT^ATT
Tth111I 2496 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1230 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3231 TCTAGA T^CTAGA
XmaCI 656 CCCGGG C^CCGGG
XmaI 656 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AatII 129 GACGTC GACGT^C
831 GACGTC
ApaI 1277 GGGCCC GGGCC^C
2735 GGGCCC
BamHI 2794 GGATCC G^GATCC
3867 GGATCC
BciVI 4202 GGATAC GTATCC(6/5)
4402 GGATAC
Bsp1407I 673 TGTACA T^GTACA
748 TGTACA
BspLU11I 31 ACATGT A^CATGT
4168 ACATGT
BspMI 1384 ACCTGC ACCTGC(4/8)
2024 ACCTGC
BsrDI 2651 CATTGC GCAATG(2/0)
106 GCAATG
BsrGI 673 TGTACA T^GTACA
748 TGTACA
BssHII 1442 GCGCGC G^CGCGC
1444 GCGCGC
BssSI 606 CACGAG CACGAG(-5/-1)
2010 CACGAG
EagI 402 CGGCCG C^GGCCG
997 CGGCCG
EclXI 402 CGGCCG C^GGCCG
997 CGGCCG
Eco52I 402 CGGCCG C^GGCCG
997 CGGCCG
KspI 631 CCGCGG CCGC^GG
893 CCGCGG
NheI 1377 GCTAGC G^CTAGC
3969 GCTAGC
PaeR7I 1602 CTCGAG C^TCGAG
2714 CTCGAG
SacII 631 CCGCGG CCGC^GG
893 CCGCGG
SgrAI 901 CGCCGGTG C[GA]^CCGG[CT]G
1063 CGCCGGTG
SphI 58 GCATGC GCATG^C
1477 GCATGC
SspBI 673 TGTACA T^GTACA
748 TGTACA
TfiI 927 GATTC G^A[AT]TC
2003 GAATC
XhoI 1602 CTCGAG C^TCGAG
2714 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI Aor51HI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaHI BsaI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BsrBI BsrFI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco47III EcoNI
EcoO109I EcoO65I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII