################################## # NO CUT ENZYMES ( 53 enzymes) # ################################## Acc65I AccI AclI AgeI AscI AseI AsnI Asp700I Asp718I AvrII BbrPI BcgI BclI BlnI BsaBI BsiWI BsmI BspDI BspHI Bst1107I BstZ17I ClaI DrdI EcoT22I FbaI HincII HindII HindIII HpaI KpnI MamI MfeI MunI NdeI NotI NruI NsiI PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI SalI SexAI SfiI SpeI SwaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccIII 226 TCCGGA T^CCGGA AcsI 3214 GAATTC [GA]^AATT[CT] AflII 63 CTTAAG C^TTAAG AhdI 4342 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 429 GTGCAC G^TGCAC AocI 2295 CCTAAGG CC^T[ACGT]AGG ApaLI 429 GTGCAC G^TGCAC ApoI 3214 GAATTC [GA]^AATT[CT] AspEI 4342 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 2496 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1408 TGCGCA TGC^GCA BalI 1391 TGGCCA TGG^CCA BbvCI 1222 CCTCAGC CCTCAGC(-5/-2) BfrI 63 CTTAAG C^TTAAG BglII 163 AGATCT A^GATCT BsaAI 5053 TACGTA [CT]AC^GT[GA] BseAI 226 TCCGGA T^CCGGA BspEI 226 TCCGGA T^CCGGA BstAPI 4306 GCATGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 3786 TTCGAA TT^CGAA Bsu36I 2295 CCTAAGG CC^T[ACGT]AGG CpoI 3127 CGGTCCG CG^G[AT]CCG DraIII 3917 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 4342 GACCACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2295 CCTAAGG CC^T[ACGT]AGG EcoRI 3214 GAATTC G^AATTC EcoRV 138 GATATC GAT^ATC FseI 399 GGCCGGCC GGCCGG^CC FspI 1408 TGCGCA TGC^GCA MluI 244 ACGCGT A^CGCGT MluNI 1391 TGGCCA TGG^CCA MroI 226 TCCGGA T^CCGGA MscI 1391 TGGCCA TGG^CCA NspV 3786 TTCGAA TT^CGAA PflFI 2496 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1230 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsrII 3127 CGGTCCG CG^G[AT]CCG SapI 4377 GAAGAGC GCTCTTC(1/4) SbfI 1308 CCTGCAGG CCTGCA^GG ScaI 4389 AGTACT AGT^ACT SfuI 3786 TTCGAA TT^CGAA SmaI 656 CCCGGG CCC^GGG SnaBI 5053 TACGTA TAC^GTA Sse8387I 1308 CCTGCAGG CCTGCA^GG SspI 5074 AATATT AAT^ATT Tth111I 2496 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1230 CCAGTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 3231 TCTAGA T^CTAGA XmaCI 656 CCCGGG C^CCGGG XmaI 656 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AatII 129 GACGTC GACGT^C 831 GACGTC ApaI 1277 GGGCCC GGGCC^C 2735 GGGCCC BamHI 2794 GGATCC G^GATCC 3867 GGATCC BciVI 4202 GGATAC GTATCC(6/5) 4402 GGATAC Bsp1407I 673 TGTACA T^GTACA 748 TGTACA BspLU11I 31 ACATGT A^CATGT 4168 ACATGT BspMI 1384 ACCTGC ACCTGC(4/8) 2024 ACCTGC BsrDI 2651 CATTGC GCAATG(2/0) 106 GCAATG BsrGI 673 TGTACA T^GTACA 748 TGTACA BssHII 1442 GCGCGC G^CGCGC 1444 GCGCGC BssSI 606 CACGAG CACGAG(-5/-1) 2010 CACGAG EagI 402 CGGCCG C^GGCCG 997 CGGCCG EclXI 402 CGGCCG C^GGCCG 997 CGGCCG Eco52I 402 CGGCCG C^GGCCG 997 CGGCCG KspI 631 CCGCGG CCGC^GG 893 CCGCGG NheI 1377 GCTAGC G^CTAGC 3969 GCTAGC PaeR7I 1602 CTCGAG C^TCGAG 2714 CTCGAG SacII 631 CCGCGG CCGC^GG 893 CCGCGG SgrAI 901 CGCCGGTG C[GA]^CCGG[CT]G 1063 CGCCGGTG SphI 58 GCATGC GCATG^C 1477 GCATGC SspBI 673 TGTACA T^GTACA 748 TGTACA TfiI 927 GATTC G^A[AT]TC 2003 GAATC XhoI 1602 CTCGAG C^TCGAG 2714 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 132 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI AlwNI Aor51HI AspHI AvaI AvaII BanI BanII BbeI BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco47III EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII