##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccIII AclI AcyI AhdI
Aor51HI AscI AspEI AspI AviII
AvrII BbeI BcgI BglI BlnI
BlpI Bpu1102I BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BsrBI BssHII BssSI Bst1107I BstEII
BstPI BstXI BstZ17I CelII ClaI
CpoI DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
FseI FspI Hin1I HindIII KasI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NcoI NgoMIV
NheI NotI NruI PacI PflFI
PmeI PpuMI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SnaBI SphI
Sse8387I SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Acc65I 804 GGTACC G^GTACC
AccI 104 GTCTAC GT^[AC][GT]AC
AccII 3439 CGCG CG^CG
AgeI 160 ACCGGT A^CCGGT
ApaI 1862 GGGCCC GGGCC^C
Asp718I 804 GGTACC G^GTACC
BalI 1534 TGGCCA TGG^CCA
BamHI 828 GGATCC G^GATCC
BbrPI 2231 CACGTG CAC^GTG
BclI 3738 TGATCA T^GATCA
BsaAI 2231 CACGTG [CT]AC^GT[GA]
Bsp1407I 490 TGTACA T^GTACA
BspHI 2760 TCATGA T^CATGA
BsrGI 490 TGTACA T^GTACA
BstAPI 3222 GCAAGGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3903 TTCGAA TT^CGAA
BstUI 3439 CGCG CG^CG
DraII 1861 GGGGCCC [GA]G^G[ACGT]CC[CT]
DraIII 3070 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1534 TGGCCA [CT]^GGCC[GA]
EcoO109I 1861 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1928 GAATTC G^AATTC
FauI 5299 GCGGG CCCGC(4/6)
FbaI 3738 TGATCA T^GATCA
HaeII 1375 AGCGCC [GA]GCGC^[CT]
KpnI 804 GGTACC GGTAC^C
MluNI 1534 TGGCCA TGG^CCA
MscI 1534 TGGCCA TGG^CCA
MvnI 3439 CGCG CG^CG
NspV 3903 TTCGAA TT^CGAA
PaeR7I 758 CTCGAG C^TCGAG
PflMI 1784 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 160 ACCGGT A^CCGGT
PmaCI 2231 CACGTG CAC^GTG
PmlI 2231 CACGTG CAC^GTG
RcaI 2760 TCATGA T^CATGA
SfuI 3903 TTCGAA TT^CGAA
SmaI 5018 CCCGGG CCC^GGG
SspBI 490 TGTACA T^GTACA
StuI 3007 AGGCCT AGG^CCT
Van91I 1784 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 4710 TCTAGA T^CTAGA
XhoI 758 CTCGAG C^TCGAG
XmaCI 5018 CCCGGG C^CCGGG
XmaI 5018 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Alw44I 937 GTGCAC G^TGCAC
1147 GTGCAC
ApaLI 937 GTGCAC G^TGCAC
1147 GTGCAC
AvaI 758 CTCGAG C^[CT]CG[GA]G
5018 CCCGGG
BciVI 3353 GTATCC GTATCC(6/5)
5204 GTATCC
BglII 2030 AGATCT A^GATCT
4675 AGATCT
BsaWI 160 ACCGGT [AT]^CCGG[AT]
4035 ACCGGA
BsoBI 758 CTCGAG C^[CT]CG[GA]G
5018 CCCGGG
CfoI 1376 GCGC GCG^C
3438 GCGC
DraI 3376 TTTAAA TTT^AAA
4774 TTTAAA
EcoNI 4231 CCTAGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4809 CCTTTTATAGG
HgaI 4579 GACGC GACGC(5/10)
2851 GCGTC
HhaI 1376 GCGC GCG^C
3438 GCGC
HinP1I 1376 GCGC G^CGC
3438 GCGC
HpaI 741 GTTAAC GTT^AAC
3138 GTTAAC
PshAI 608 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1181 GACCACCGTC
PstI 890 CTGCAG CTGCA^G
1079 CTGCAG
SapI 3217 GAAGAGC GCTCTTC(1/4)
1975 GCTCTTC
XcmI 1890 CCAGATCCTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5208 CCAAGGGCCAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AflII AflIII
AluI AlwI AlwNI AocI ApoI
AseI AsnI Asp700I AspHI AvaII
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BfrI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspLU11I BspMI BsrDI
BsrFI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I Cfr10I Cfr13I
DdeI DpnII EarI Eco81I EcoRII
EcoT14I EcoT22I Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeI NdeII NlaIII
NlaIV NsiI NspI PleI PshBI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SpeI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI