################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AatII AccIII AclI AcyI AhdI Aor51HI AscI AspEI AspI AviII AvrII BbeI BcgI BglI BlnI BlpI Bpu1102I BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BspDI BspEI BsrBI BssHII BssSI Bst1107I BstEII BstPI BstXI BstZ17I CelII ClaI CpoI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV FseI FspI Hin1I HindIII KasI KspI MamI MfeI MluI MroI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI PacI PflFI PmeI PpuMI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI SphI Sse8387I SwaI Tth111I ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Acc65I 804 GGTACC G^GTACC AccI 104 GTCTAC GT^[AC][GT]AC AccII 3439 CGCG CG^CG AgeI 160 ACCGGT A^CCGGT ApaI 1862 GGGCCC GGGCC^C Asp718I 804 GGTACC G^GTACC BalI 1534 TGGCCA TGG^CCA BamHI 828 GGATCC G^GATCC BbrPI 2231 CACGTG CAC^GTG BclI 3738 TGATCA T^GATCA BsaAI 2231 CACGTG [CT]AC^GT[GA] Bsp1407I 490 TGTACA T^GTACA BspHI 2760 TCATGA T^CATGA BsrGI 490 TGTACA T^GTACA BstAPI 3222 GCAAGGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 3903 TTCGAA TT^CGAA BstUI 3439 CGCG CG^CG DraII 1861 GGGGCCC [GA]G^G[ACGT]CC[CT] DraIII 3070 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 1534 TGGCCA [CT]^GGCC[GA] EcoO109I 1861 GGGGCCC [GA]G^G[ACGT]CC[CT] EcoRI 1928 GAATTC G^AATTC FauI 5299 GCGGG CCCGC(4/6) FbaI 3738 TGATCA T^GATCA HaeII 1375 AGCGCC [GA]GCGC^[CT] KpnI 804 GGTACC GGTAC^C MluNI 1534 TGGCCA TGG^CCA MscI 1534 TGGCCA TGG^CCA MvnI 3439 CGCG CG^CG NspV 3903 TTCGAA TT^CGAA PaeR7I 758 CTCGAG C^TCGAG PflMI 1784 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 160 ACCGGT A^CCGGT PmaCI 2231 CACGTG CAC^GTG PmlI 2231 CACGTG CAC^GTG RcaI 2760 TCATGA T^CATGA SfuI 3903 TTCGAA TT^CGAA SmaI 5018 CCCGGG CCC^GGG SspBI 490 TGTACA T^GTACA StuI 3007 AGGCCT AGG^CCT Van91I 1784 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 4710 TCTAGA T^CTAGA XhoI 758 CTCGAG C^TCGAG XmaCI 5018 CCCGGG C^CCGGG XmaI 5018 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### Alw44I 937 GTGCAC G^TGCAC 1147 GTGCAC ApaLI 937 GTGCAC G^TGCAC 1147 GTGCAC AvaI 758 CTCGAG C^[CT]CG[GA]G 5018 CCCGGG BciVI 3353 GTATCC GTATCC(6/5) 5204 GTATCC BglII 2030 AGATCT A^GATCT 4675 AGATCT BsaWI 160 ACCGGT [AT]^CCGG[AT] 4035 ACCGGA BsoBI 758 CTCGAG C^[CT]CG[GA]G 5018 CCCGGG CfoI 1376 GCGC GCG^C 3438 GCGC DraI 3376 TTTAAA TTT^AAA 4774 TTTAAA EcoNI 4231 CCTAGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4809 CCTTTTATAGG HgaI 4579 GACGC GACGC(5/10) 2851 GCGTC HhaI 1376 GCGC GCG^C 3438 GCGC HinP1I 1376 GCGC G^CGC 3438 GCGC HpaI 741 GTTAAC GTT^AAC 3138 GTTAAC PshAI 608 GACAGCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1181 GACCACCGTC PstI 890 CTGCAG CTGCA^G 1079 CTGCAG SapI 3217 GAAGAGC GCTCTTC(1/4) 1975 GCTCTTC XcmI 1890 CCAGATCCTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 5208 CCAAGGGCCAGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AciI AcsI AfaI AflII AflIII AluI AlwI AlwNI AocI ApoI AseI AsnI Asp700I AspHI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BfrI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspLU11I BspMI BsrDI BsrFI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I Cfr10I Cfr13I DdeI DpnII EarI Eco81I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV NsiI NspI PleI PshBI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SpeI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI