##################################
# NO CUT ENZYMES (   83 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AhdI        
Aor51HI     AscI        AspEI       AspI        AviII       
AvrII       BbeI        BcgI        BglI        BlnI        
BlpI        Bpu1102I    BsaBI       BsaHI       BseAI       
BsiEI       BsiWI       BsmBI       BspDI       BspEI       
BsrBI       BssHII      BssSI       Bst1107I    BstEII      
BstPI       BstXI       BstZ17I     CelII       ClaI        
CpoI        DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRV       
FseI        FspI        Hin1I       HindIII     KasI        
KspI        MamI        MfeI        MluI        MroI        
MunI        NaeI        NarI        NcoI        NgoMIV      
NheI        NotI        NruI        PacI        PflFI       
PmeI        PpuMI       Psp1406I    PvuI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SgrAI       SnaBI       SphI        
Sse8387I    SwaI        Tth111I     


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

Acc65I        804  GGTACC               G^GTACC
AccI          104  GTCTAC               GT^[AC][GT]AC
AccII        3439  CGCG                 CG^CG
AgeI          160  ACCGGT               A^CCGGT
ApaI         1862  GGGCCC               GGGCC^C
Asp718I       804  GGTACC               G^GTACC
BalI         1534  TGGCCA               TGG^CCA
BamHI         828  GGATCC               G^GATCC
BbrPI        2231  CACGTG               CAC^GTG
BclI         3738  TGATCA               T^GATCA
BsaAI        2231  CACGTG               [CT]AC^GT[GA]
Bsp1407I      490  TGTACA               T^GTACA
BspHI        2760  TCATGA               T^CATGA
BsrGI         490  TGTACA               T^GTACA
BstAPI       3222  GCAAGGTATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        3903  TTCGAA               TT^CGAA
BstUI        3439  CGCG                 CG^CG
DraII        1861  GGGGCCC              [GA]G^G[ACGT]CC[CT]
DraIII       3070  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
EaeI         1534  TGGCCA               [CT]^GGCC[GA]
EcoO109I     1861  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoRI        1928  GAATTC               G^AATTC
FauI         5299  GCGGG                CCCGC(4/6)
FbaI         3738  TGATCA               T^GATCA
HaeII        1375  AGCGCC               [GA]GCGC^[CT]
KpnI          804  GGTACC               GGTAC^C
MluNI        1534  TGGCCA               TGG^CCA
MscI         1534  TGGCCA               TGG^CCA
MvnI         3439  CGCG                 CG^CG
NspV         3903  TTCGAA               TT^CGAA
PaeR7I        758  CTCGAG               C^TCGAG
PflMI        1784  CCAGCCAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI         160  ACCGGT               A^CCGGT
PmaCI        2231  CACGTG               CAC^GTG
PmlI         2231  CACGTG               CAC^GTG
RcaI         2760  TCATGA               T^CATGA
SfuI         3903  TTCGAA               TT^CGAA
SmaI         5018  CCCGGG               CCC^GGG
SspBI         490  TGTACA               T^GTACA
StuI         3007  AGGCCT               AGG^CCT
Van91I       1784  CCAGCCAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         4710  TCTAGA               T^CTAGA
XhoI          758  CTCGAG               C^TCGAG
XmaCI        5018  CCCGGG               C^CCGGG
XmaI         5018  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   18 enzymes) #
###################################

Alw44I        937  GTGCAC               G^TGCAC
             1147  GTGCAC              
ApaLI         937  GTGCAC               G^TGCAC
             1147  GTGCAC              
AvaI          758  CTCGAG               C^[CT]CG[GA]G
             5018  CCCGGG              
BciVI        3353  GTATCC               GTATCC(6/5)
             5204  GTATCC              
BglII        2030  AGATCT               A^GATCT
             4675  AGATCT              
BsaWI         160  ACCGGT               [AT]^CCGG[AT]
             4035  ACCGGA              
BsoBI         758  CTCGAG               C^[CT]CG[GA]G
             5018  CCCGGG              
CfoI         1376  GCGC                 GCG^C
             3438  GCGC                
DraI         3376  TTTAAA               TTT^AAA
             4774  TTTAAA              
EcoNI        4231  CCTAGCTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             4809  CCTTTTATAGG         
HgaI         4579  GACGC                GACGC(5/10)
             2851  GCGTC               
HhaI         1376  GCGC                 GCG^C
             3438  GCGC                
HinP1I       1376  GCGC                 G^CGC
             3438  GCGC                
HpaI          741  GTTAAC               GTT^AAC
             3138  GTTAAC              
PshAI         608  GACAGCCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1181  GACCACCGTC          
PstI          890  CTGCAG               CTGCA^G
             1079  CTGCAG              
SapI         3217  GAAGAGC              GCTCTTC(1/4)
             1975  GCTCTTC             
XcmI         1890  CCAGATCCTGCTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             5208  CCAAGGGCCAGCTGG     


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AciI        AcsI        AfaI        AflII       AflIII      
AluI        AlwI        AlwNI       AocI        ApoI        
AseI        AsnI        Asp700I     AspHI       AvaII       
BanI        BanII       BbsI        BbvCI       BbvI        
BcnI        BfaI        BfrI        BmyI        BpmI        
BpuAI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        Bsp1286I    BspLU11I    BspMI       BsrDI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Bsu36I      Cac8I       Cfr10I      Cfr13I      
DdeI        DpnII       EarI        Eco81I      EcoRII      
EcoT14I     EcoT22I     Fnu4HI      FokI        HaeIII      
HapII       HincII      HindII      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MflI        MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeI        NdeII       NlaIII      
NlaIV       NsiI        NspI        PleI        PshBI       
PvuII       RsaI        SacI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        SpeI        
SspI        StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       XmnI