##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AccIII AclI AhdI AscI AseI
AsnI Asp700I AspEI BbeI BbrPI
BcgI BclI BglI BglII BseAI
BsiEI BsiWI BspDI BspEI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco52I
EcoT22I FbaI FseI HpaI KasI
KspI MfeI MluI MroI MunI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspV PacI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SbfI SfiI
SfoI SfuI SmaI SnaBI SpeI
Sse8387I SwaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 2650 GACGTC GACGT^C
AccII 2359 CGCG CG^CG
AflII 3405 CTTAAG C^TTAAG
AgeI 5021 ACCGGT A^CCGGT
Aor51HI 515 AGCGCT AGC^GCT
AspI 1407 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 32 TGCGCA TGC^GCA
AvrII 1032 CCTAGG C^CTAGG
BfrI 3405 CTTAAG C^TTAAG
BlnI 1032 CCTAGG C^CTAGG
BsaAI 961 TACGTG [CT]AC^GT[GA]
BsaBI 1002 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 2811 GCATTC GAATGC(1/-1)
Bsp1407I 5662 TGTACA T^GTACA
BspHI 5188 TCATGA T^CATGA
BsrGI 5662 TGTACA T^GTACA
BstAPI 1100 GCATTGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 2359 CGCG CG^CG
DraIII 762 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 515 AGCGCT AGC^GCT
EcoNI 5298 CCTCCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1796 GATATC GAT^ATC
FspI 32 TGCGCA TGC^GCA
HincII 1174 GTCGAC GT[CT]^[GA]AC
HindII 1174 GTCGAC GT[CT]^[GA]AC
HindIII 4075 AAGCTT A^AGCTT
MamI 1002 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 2359 CGCG CG^CG
NheI 4542 GCTAGC G^CTAGC
PflFI 1407 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 5021 ACCGGT A^CCGGT
RcaI 5188 TCATGA T^CATGA
SacI 2277 GAGCTC GAGCT^C
SalI 1174 GTCGAC G^TCGAC
SexAI 4097 ACCAGGT A^CC[AT]GGT
SgrAI 5020 CACCGGTG C[GA]^CCGG[CT]G
SspBI 5662 TGTACA T^GTACA
Tth111I 1407 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 5537 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BamHI 421 GGATCC G^GATCC
4153 GGATCC
BbvCI 306 CCTCAGC CCTCAGC(-5/-2)
4278 GCTGAGG
BciVI 3009 GGATAC GTATCC(6/5)
4883 GTATCC
BsaWI 4905 ACCGGA [AT]^CCGG[AT]
5021 ACCGGT
BsmBI 2652 CGTCTC CGTCTC(1/5)
1405 GAGACG
BsrBI 312 CCGCTC CCGCTC(-3/-3)
1340 GAGCGG
BsrDI 2390 CATTGC GCAATG(2/0)
5761 CATTGC
BssSI 2582 CACGAG CACGAG(-5/-1)
2183 CTCGTG
BstEII 972 GGTCACC G^GT[ACGT]ACC
4737 GGTGACC
BstPI 972 GGTCACC G^GT[ACGT]ACC
4737 GGTGACC
EcoO65I 972 GGTCACC G^GT[ACGT]ACC
4737 GGTGACC
EcoRI 1080 GAATTC G^AATTC
4362 GAATTC
HaeII 515 AGCGCT [GA]GCGC^[CT]
1888 AGCGCC
ScaI 2773 AGTACT AGT^ACT
5546 AGTACT
SphI 771 GCATGC GCATG^C
3722 GCATGC
SspI 1701 AATATT AAT^ATT
4588 AATATT
TfiI 2792 GAATC G^A[AT]TC
5534 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
Acc65I AccI AciI AcsI AcyI
AfaI AflIII AluI Alw44I AlwI
AlwNI AocI ApaI ApaLI ApoI
Asp718I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspLU11I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KpnI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PaeR7I PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoI XhoII