##################################
# NO CUT ENZYMES ( 43 enzymes) #
##################################
Aor51HI AviII AvrII BamHI BlnI
BsaBI BsiWI BssSI BstBI CpoI
Eco47III EcoRI EcoRV EcoT22I FseI
FspI HpaI KspI MamI MfeI
MluI MunI NheI NotI NruI
NsiI NspV PacI PaeR7I PmeI
PvuI RsrII SacII SapI SbfI
ScaI SexAI SfuI SnaBI SpeI
Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 3163 GACGTC GACGT^C
AccIII 379 TCCGGA T^CCGGA
AclI 1070 AACGTT AA^CGTT
AgeI 577 ACCGGT A^CCGGT
AhdI 1466 GACCTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 346 GGATC GGATC(4/5)
AscI 126 GGCGCGCC GG^CGCGCC
AspEI 1466 GACCTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbrPI 1838 CACGTG CAC^GTG
BbvCI 3113 CCTCAGC CCTCAGC(-5/-2)
BcgI 2170 GCAGCCCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1029 GGATAC GTATCC(6/5)
BclI 284 TGATCA T^GATCA
BsaAI 1838 CACGTG [CT]AC^GT[GA]
BseAI 379 TCCGGA T^CCGGA
BsiEI 473 CGGCCG CG[GA][CT]^CG
BspDI 2178 ATCGAT AT^CGAT
BspEI 379 TCCGGA T^CCGGA
BspHI 2626 TCATGA T^CATGA
BssHII 127 GCGCGC G^CGCGC
BstEII 5481 GGTCACC G^GT[ACGT]ACC
BstPI 5481 GGTCACC G^GT[ACGT]ACC
ClaI 2178 ATCGAT AT^CGAT
DrdI 1951 GACCCTTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 473 CGGCCG C^GGCCG
Eam1105I 1466 GACCTATGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 473 CGGCCG C^GGCCG
Eco52I 473 CGGCCG C^GGCCG
EcoNI 1782 CCTGTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 5481 GGTCACC G^GT[ACGT]ACC
FbaI 284 TGATCA T^GATCA
HincII 3377 GTCGAC GT[CT]^[GA]AC
HindII 3377 GTCGAC GT[CT]^[GA]AC
HindIII 3300 AAGCTT A^AGCTT
MroI 379 TCCGGA T^CCGGA
NdeI 2399 CATATG CA^TATG
PinAI 577 ACCGGT A^CCGGT
PmaCI 1838 CACGTG CAC^GTG
PmlI 1838 CACGTG CAC^GTG
Psp1406I 1070 AACGTT AA^CGTT
RcaI 2626 TCATGA T^CATGA
SalI 3377 GTCGAC G^TCGAC
SfiI 1200 GGCCTGCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 617 CACCGGCG C[GA]^CCGG[CT]G
SphI 466 GCATGC GCATG^C
StuI 2230 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 2571 GGTACC G^GTACC
3076 GGTACC
AocI 1194 CCTGAGG CC^T[ACGT]AGG
3532 CCTGAGG
AseI 4636 ATTAAT AT^TAAT
6515 ATTAAT
AsnI 4636 ATTAAT AT^TAAT
6515 ATTAAT
Asp700I 4292 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4808 GAATGATTTC
Asp718I 2571 GGTACC G^GTACC
3076 GGTACC
AspI 1779 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2559 GACCACGTC
BalI 947 TGGCCA TGG^CCA
1304 TGGCCA
BglII 289 AGATCT A^GATCT
6470 AGATCT
BlpI 3126 GCTGAGC GC^T[ACGT]AGC
5219 GCTTAGC
Bpu1102I 3126 GCTGAGC GC^T[ACGT]AGC
5219 GCTTAGC
BsaI 3349 GGTCTC GGTCTC(1/5)
4645 GGTCTC
BsmBI 4170 CGTCTC CGTCTC(1/5)
5961 CGTCTC
BspLU11I 5839 ACATGT A^CATGT
6435 ACATGT
BstYI 289 AGATCT [GA]^GATC[CT]
6470 AGATCT
Bsu36I 1194 CCTGAGG CC^T[ACGT]AGG
3532 CCTGAGG
CelII 3126 GCTGAGC GC^T[ACGT]AGC
5219 GCTTAGC
DraI 4697 TTTAAA TTT^AAA
6594 TTTAAA
Eco81I 1194 CCTGAGG CC^T[ACGT]AGG
3532 CCTGAGG
HgaI 4169 GCGTC GACGC(5/10)
5960 GCGTC
KpnI 2571 GGTACC GGTAC^C
3076 GGTACC
MflI 289 AGATCT [GA]^GATC[CT]
6470 AGATCT
MluNI 947 TGGCCA TGG^CCA
1304 TGGCCA
MscI 947 TGGCCA TGG^CCA
1304 TGGCCA
NaeI 18 GCCGGC GCC^GGC
1332 GCCGGC
NgoMIV 18 GCCGGC G^CCGGC
1332 GCCGGC
PflFI 1779 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2559 GACCACGTC
PshAI 1041 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3159 GACAGACGTC
PshBI 4636 ATTAAT AT^TAAT
6515 ATTAAT
SacI 820 GAGCTC GAGCT^C
6216 GAGCTC
Tth111I 1779 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2559 GACCACGTC
XbaI 4804 TCTAGA T^CTAGA
5523 TCTAGA
XhoII 289 AGATCT [GA]^GATC[CT]
6470 AGATCT
XmnI 4292 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4808 GAATGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 135 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflII AflIII AluI Alw44I
AlwNI ApaI ApaLI ApoI AspHI
AvaI AvaII BanI BanII BbeI
BbsI BbvI BcnI BfaI BfrI
BglI BmyI BpmI BpuAI BsaHI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssKI
Bst1107I BstAPI BstF5I BstNI BstUI
BstXI BstZ17I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI SmaI SmlI
SspBI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XmaCI XmaI