##################################
# NO CUT ENZYMES ( 57 enzymes) #
##################################
AccIII AclI AgeI ApaI AscI
AseI AsnI AvrII BcgI BciVI
BclI BlnI BsaWI BseAI BsiWI
BspDI BspEI BspHI BssHII BstBI
ClaI CpoI DrdI EagI EclXI
Eco52I EcoRV EcoT22I FbaI FseI
HindIII HpaI KspI MfeI MroI
MunI NdeI NotI NruI NsiI
NspV PacI PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SfiI SfuI SgrAI SpeI
SspI SwaI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
AatII 2856 GACGTC GACGT^C
AflII 2565 CTTAAG C^TTAAG
Alw44I 696 GTGCAC G^TGCAC
ApaLI 696 GTGCAC G^TGCAC
BamHI 1058 GGATCC G^GATCC
BbrPI 5680 CACGTG CAC^GTG
BfrI 2565 CTTAAG C^TTAAG
BglII 5512 AGATCT A^GATCT
BsiEI 3032 CGACCG CG[GA][CT]^CG
BsmBI 5462 CGTCTC CGTCTC(1/5)
Bsp1407I 4910 TGTACA T^GTACA
BsrGI 4910 TGTACA T^GTACA
Bst1107I 5054 GTATAC GTA^TAC
BstZ17I 5054 GTATAC GTA^TAC
DraI 4236 TTTAAA TTT^AAA
DraIII 2028 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
MluI 3931 ACGCGT A^CGCGT
NheI 495 GCTAGC G^CTAGC
PmaCI 5680 CACGTG CAC^GTG
PmlI 5680 CACGTG CAC^GTG
SalI 2889 GTCGAC G^TCGAC
SbfI 879 CCTGCAGG CCTGCA^GG
ScaI 5919 AGTACT AGT^ACT
SnaBI 5821 TACGTA TAC^GTA
SphI 3502 GCATGC GCATG^C
Sse8387I 879 CCTGCAGG CCTGCA^GG
SspBI 4910 TGTACA T^GTACA
XbaI 3532 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 488 GGTACC G^GTACC
1969 GGTACC
AhdI 4440 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5853 GACGCTGTGTC
Asp718I 488 GGTACC G^GTACC
1969 GGTACC
AspEI 4440 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5853 GACGCTGTGTC
BbvCI 3822 CCTCAGC CCTCAGC(-5/-2)
1289 GCTGAGG
BlpI 1427 GCTAAGC GC^T[ACGT]AGC
2430 GCTAAGC
Bpu1102I 1427 GCTAAGC GC^T[ACGT]AGC
2430 GCTAAGC
BsaAI 5680 CACGTG [CT]AC^GT[GA]
5821 TACGTA
BsmI 3158 GAATGC GAATGC(1/-1)
1497 GCATTC
BspLU11I 31 ACATGT A^CATGT
3713 ACATGT
CelII 1427 GCTAAGC GC^T[ACGT]AGC
2430 GCTAAGC
Eam1105I 4440 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5853 GACGCTGTGTC
EcoRI 156 GAATTC G^AATTC
3489 GAATTC
KpnI 488 GGTACC GGTAC^C
1969 GGTACC
NaeI 2755 GCCGGC GCC^GGC
5436 GCCGGC
NgoMIV 2755 GCCGGC G^CCGGC
5436 GCCGGC
PaeR7I 5124 CTCGAG C^TCGAG
5331 CTCGAG
SacI 4421 GAGCTC GAGCT^C
5764 GAGCTC
SmaI 1654 CCCGGG CCC^GGG
5599 CCCGGG
XhoI 5124 CTCGAG C^TCGAG
5331 CTCGAG
XmaCI 1654 CCCGGG C^CCGGG
5599 CCCGGG
XmaI 1654 CCCGGG C^CCGGG
5599 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 151 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI Aor51HI ApoI Asp700I AspHI
AspI AvaI AvaII AviII BalI
BanI BanII BbeI BbsI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaBI BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrI BssKI
BssSI BstAPI BstEII BstF5I BstNI
BstPI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeII MaeIII MamI
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NcoI NdeII NlaIII NlaIV NspI
PflFI PflMI PleI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SfoI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Tth111I TthHB8I Van91I XcmI XhoII
XmnI