##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccIII AflII AhdI AscI
Asp718I AspEI AvaI AvrII BamHI
BbvCI BfrI BlnI BlpI Bpu1102I
BseAI BsiWI BsmBI BsoBI BspDI
BspEI BspHI BspLU11I Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraIII DrdI Eam1105I EarI
EcoO65I FseI KpnI Ksp632I MfeI
MroI MunI NaeI NgoMIV NotI
NruI NspV PacI PaeR7I PmeI
PshAI PvuI RcaI RsrII SalI
SapI SbfI ScaI SfiI SfuI
SmaI SnaBI Sse8387I StuI SwaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 3924 GTCTAC GT^[AC][GT]AC
AclI 1357 AACGTT AA^CGTT
AgeI 1966 ACCGGT A^CCGGT
Aor51HI 1595 AGCGCT AGC^GCT
ApaI 2364 GGGCCC GGGCC^C
AseI 4061 ATTAAT AT^TAAT
AsnI 4061 ATTAAT AT^TAAT
AviII 1458 TGCGCA TGC^GCA
BbeI 2653 GGCGCC GGCGC^C
BcgI 1190 GCACTGTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 160 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3670 AGATCT A^GATCT
BsmI 2555 GCATTC GAATGC(1/-1)
EagI 529 CGGCCG C^GGCCG
EclXI 529 CGGCCG C^GGCCG
Eco47III 1595 AGCGCT AGC^GCT
Eco52I 529 CGGCCG C^GGCCG
EcoRI 351 GAATTC G^AATTC
EcoT22I 4185 ATGCAT ATGCA^T
FspI 1458 TGCGCA TGC^GCA
KasI 2653 GGCGCC G^GCGCC
KspI 303 CCGCGG CCGC^GG
MluI 1895 ACGCGT A^CGCGT
NarI 2653 GGCGCC GG^CGCC
NcoI 2043 CCATGG C^CATGG
NsiI 4185 ATGCAT ATGCA^T
PinAI 1966 ACCGGT A^CCGGT
PshBI 4061 ATTAAT AT^TAAT
Psp1406I 1357 AACGTT AA^CGTT
PvuII 2673 CAGCTG CAG^CTG
SacI 754 GAGCTC GAGCT^C
SacII 303 CCGCGG CCGC^GG
SexAI 2317 ACCTGGT A^CC[AT]GGT
SfoI 2653 GGCGCC GGC^GCC
SpeI 3389 ACTAGT A^CTAGT
SphI 2522 GCATGC GCATG^C
SspI 44 AATATT AAT^ATT
XcmI 1982 CCACACGTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AatII 1117 GACGTC GACGT^C
1937 GACGTC
AocI 1264 CCTCAGG CC^T[ACGT]AGG
2623 CCTGAGG
Asp700I 581 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
822 GAAGACATTC
AspI 1321 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2316 GACCTGGTC
BalI 2593 TGGCCA TGG^CCA
3513 TGGCCA
BbrPI 445 CACGTG CAC^GTG
1985 CACGTG
BciVI 991 GGATAC GTATCC(6/5)
2602 GTATCC
BsaAI 445 CACGTG [CT]AC^GT[GA]
1985 CACGTG
BsaBI 124 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3511 GATGGCCATC
BsgI 1181 CTGCAC GTGCAG(16/14)
1841 GTGCAG
BsiEI 363 CGACCG CG[GA][CT]^CG
529 CGGCCG
BsrDI 1090 CATTGC GCAATG(2/0)
1733 GCAATG
BssHII 1505 GCGCGC G^CGCGC
1700 GCGCGC
BssSI 3616 CACGAG CACGAG(-5/-1)
673 CTCGTG
BstAPI 129 GCATCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
562 GCATCAGATGC
BstXI 1781 CCACACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2303 CCAGCGCAGTGG
Bsu36I 1264 CCTCAGG CC^T[ACGT]AGG
2623 CCTGAGG
Eco81I 1264 CCTCAGG CC^T[ACGT]AGG
2623 CCTGAGG
EcoRV 2167 GATATC GAT^ATC
4144 GATATC
EcoT14I 296 CCAAGG C^C[AT][AT]GG
2043 CCATGG
HpaI 3114 GTTAAC GTT^AAC
3393 GTTAAC
MamI 124 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3511 GATGGCCATC
MluNI 2593 TGGCCA TGG^CCA
3513 TGGCCA
MscI 2593 TGGCCA TGG^CCA
3513 TGGCCA
NheI 1408 GCTAGC G^CTAGC
4239 GCTAGC
PflFI 1321 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2316 GACCTGGTC
PflMI 982 CCAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2934 CCACCAAATGG
PmaCI 445 CACGTG CAC^GTG
1985 CACGTG
PmlI 445 CACGTG CAC^GTG
1985 CACGTG
SgrAI 1965 CACCGGTG C[GA]^CCGG[CT]G
2655 CGCCGGTG
StyI 296 CCAAGG C^C[AT][AT]GG
2043 CCATGG
TaqI 362 TCGA T^CGA
1606 TCGA
Tth111I 1321 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
2316 GACCTGGTC
TthHB8I 362 TCGA T^CGA
1606 TCGA
Van91I 982 CCAATCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2934 CCACCAAATGG
XbaI 789 TCTAGA T^CTAGA
4275 TCTAGA
XmnI 581 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
822 GAAGACATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 115 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
ApaLI ApoI AspHI AvaII BanI
BanII BbsI BbvI BclI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspMI BsrBI BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EcoNI EcoO109I EcoRII FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HindIII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII