##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
Acc65I AccIII AgeI AhdI AocI
AscI AseI AsnI Asp718I AspEI
BbeI BbvCI BcgI BciVI BsaWI
BseAI BspDI BspEI BspHI Bst1107I
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI Eam1105I Eco81I EcoO65I FseI
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NotI PacI PaeR7I PinAI
PmeI PshAI PshBI PstI PvuI
RcaI RsrII SacII SalI SbfI
SfiI SfoI SnaBI Sse8387I SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 2592 GACGTC GACGT^C
AccI 2990 GTAGAC GT^[AC][GT]AC
AcyI 2592 GACGTC G[GA]^CG[CT]C
ApaI 2577 GGGCCC GGGCC^C
AspI 712 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 4090 TGCGCA TGC^GCA
BbrPI 4006 CACGTG CAC^GTG
BclI 2460 TGATCA T^GATCA
BglI 85 GCCTAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 592 AGATCT A^GATCT
BsaHI 2592 GACGTC G[GA]^CG[CT]C
BsiEI 1093 CGGCCG CG[GA][CT]^CG
BsmBI 346 GAGACG CGTCTC(1/5)
BsmI 736 GAATGC GAATGC(1/-1)
BspLU11I 3807 ACATGT A^CATGT
BsrFI 1133 ACCGGC [GA]^CCGG[CT]
BssSI 1325 CACGAG CACGAG(-5/-1)
BstBI 770 TTCGAA TT^CGAA
Cfr10I 1133 ACCGGC [GA]^CCGG[CT]
DraIII 1156 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 1093 CGGCCG C^GGCCG
EclXI 1093 CGGCCG C^GGCCG
Eco52I 1093 CGGCCG C^GGCCG
EcoNI 2353 CCTTCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 704 GAATTC G^AATTC
EcoRV 1950 GATATC GAT^ATC
EcoT22I 4041 ATGCAT ATGCA^T
FbaI 2460 TGATCA T^GATCA
FspI 4090 TGCGCA TGC^GCA
Hin1I 2592 GACGTC G[GA]^CG[CT]C
NcoI 2581 CCATGG C^CATGG
NdeI 2120 CATATG CA^TATG
NheI 314 GCTAGC G^CTAGC
NsiI 4041 ATGCAT ATGCA^T
NspV 770 TTCGAA TT^CGAA
PflFI 712 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 3320 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 4006 CACGTG CAC^GTG
PmlI 4006 CACGTG CAC^GTG
PpuMI 3166 AGGTCCT [GA]G^G[AT]CC[CT]
ScaI 3295 AGTACT AGT^ACT
SexAI 1443 ACCTGGT A^CC[AT]GGT
SfuI 770 TTCGAA TT^CGAA
SgrAI 1132 CACCGGCG C[GA]^CCGG[CT]G
SmaI 1908 CCCGGG CCC^GGG
SpeI 3648 ACTAGT A^CTAGT
SspI 4519 AATATT AAT^ATT
Tth111I 712 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 3320 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1 CCAGCCCGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1908 CCCGGG C^CCGGG
XmaI 1908 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AclI 126 AACGTT AA^CGTT
304 AACGTT
AflII 1966 CTTAAG C^TTAAG
3089 CTTAAG
AlwNI 103 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
2932 CAGAATCTG
Aor51HI 1350 AGCGCT AGC^GCT
1861 AGCGCT
AvrII 86 CCTAGG C^CTAGG
244 CCTAGG
BalI 540 TGGCCA TGG^CCA
3622 TGGCCA
BamHI 577 GGATCC G^GATCC
2664 GGATCC
BfrI 1966 CTTAAG C^TTAAG
3089 CTTAAG
BlnI 86 CCTAGG C^CTAGG
244 CCTAGG
BlpI 1857 GCTCAGC GC^T[ACGT]AGC
4304 GCTGAGC
Bpu1102I 1857 GCTCAGC GC^T[ACGT]AGC
4304 GCTGAGC
BsaAI 133 CACGTA [CT]AC^GT[GA]
4006 CACGTG
BsaBI 2605 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2967 GATGTAGATC
BsiWI 1230 CGTACG C^GTACG
1566 CGTACG
BspMI 855 ACCTGC ACCTGC(4/8)
2290 GCAGGT
BsrDI 3886 CATTGC GCAATG(2/0)
3958 CATTGC
BssHII 3442 GCGCGC G^CGCGC
3444 GCGCGC
BstXI 3370 CCAGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4228 CCAGCTGGGTGG
CelII 1857 GCTCAGC GC^T[ACGT]AGC
4304 GCTGAGC
DraI 3465 TTTAAA TTT^AAA
4545 TTTAAA
DrdI 854 GACCTGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3159 GACACTCAGGTC
DsaI 797 CCGTGG C^C[GA][CT]GG
2581 CCATGG
Eco47III 1350 AGCGCT AGC^GCT
1861 AGCGCT
HaeII 1350 AGCGCT [GA]GCGC^[CT]
1861 AGCGCT
HgaI 958 GACGC GACGC(5/10)
1042 GACGC
MamI 2605 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2967 GATGTAGATC
MluNI 540 TGGCCA TGG^CCA
3622 TGGCCA
MscI 540 TGGCCA TGG^CCA
3622 TGGCCA
NruI 176 TCGCGA TCG^CGA
708 TCGCGA
Psp1406I 126 AACGTT AA^CGTT
304 AACGTT
PvuII 3628 CAGCTG CAG^CTG
4229 CAGCTG
SacI 1771 GAGCTC GAGCT^C
1855 GAGCTC
SapI 722 GAAGAGC GCTCTTC(1/4)
1588 GAAGAGC
SphI 2896 GCATGC GCATG^C
4353 GCATGC
StuI 765 AGGCCT AGG^CCT
2196 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI Alw44I AlwI ApaLI ApoI
Asp700I AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BsrBI BsrGI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HincII HindII HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI