################################## # NO CUT ENZYMES ( 62 enzymes) # ################################## Acc65I AccIII AgeI AhdI AocI AscI AseI AsnI Asp718I AspEI BbeI BbvCI BcgI BciVI BsaWI BseAI BspDI BspEI BspHI Bst1107I BstEII BstPI BstZ17I Bsu36I ClaI CpoI Eam1105I Eco81I EcoO65I FseI HpaI KasI KpnI KspI MfeI MluI MroI MunI NaeI NarI NgoMIV NotI PacI PaeR7I PinAI PmeI PshAI PshBI PstI PvuI RcaI RsrII SacII SalI SbfI SfiI SfoI SnaBI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 2592 GACGTC GACGT^C AccI 2990 GTAGAC GT^[AC][GT]AC AcyI 2592 GACGTC G[GA]^CG[CT]C ApaI 2577 GGGCCC GGGCC^C AspI 712 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 4090 TGCGCA TGC^GCA BbrPI 4006 CACGTG CAC^GTG BclI 2460 TGATCA T^GATCA BglI 85 GCCTAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 592 AGATCT A^GATCT BsaHI 2592 GACGTC G[GA]^CG[CT]C BsiEI 1093 CGGCCG CG[GA][CT]^CG BsmBI 346 GAGACG CGTCTC(1/5) BsmI 736 GAATGC GAATGC(1/-1) BspLU11I 3807 ACATGT A^CATGT BsrFI 1133 ACCGGC [GA]^CCGG[CT] BssSI 1325 CACGAG CACGAG(-5/-1) BstBI 770 TTCGAA TT^CGAA Cfr10I 1133 ACCGGC [GA]^CCGG[CT] DraIII 1156 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 1093 CGGCCG C^GGCCG EclXI 1093 CGGCCG C^GGCCG Eco52I 1093 CGGCCG C^GGCCG EcoNI 2353 CCTTCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 704 GAATTC G^AATTC EcoRV 1950 GATATC GAT^ATC EcoT22I 4041 ATGCAT ATGCA^T FbaI 2460 TGATCA T^GATCA FspI 4090 TGCGCA TGC^GCA Hin1I 2592 GACGTC G[GA]^CG[CT]C NcoI 2581 CCATGG C^CATGG NdeI 2120 CATATG CA^TATG NheI 314 GCTAGC G^CTAGC NsiI 4041 ATGCAT ATGCA^T NspV 770 TTCGAA TT^CGAA PflFI 712 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 3320 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 4006 CACGTG CAC^GTG PmlI 4006 CACGTG CAC^GTG PpuMI 3166 AGGTCCT [GA]G^G[AT]CC[CT] ScaI 3295 AGTACT AGT^ACT SexAI 1443 ACCTGGT A^CC[AT]GGT SfuI 770 TTCGAA TT^CGAA SgrAI 1132 CACCGGCG C[GA]^CCGG[CT]G SmaI 1908 CCCGGG CCC^GGG SpeI 3648 ACTAGT A^CTAGT SspI 4519 AATATT AAT^ATT Tth111I 712 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 3320 CCAGATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1 CCAGCCCGGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 1908 CCCGGG C^CCGGG XmaI 1908 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AclI 126 AACGTT AA^CGTT 304 AACGTT AflII 1966 CTTAAG C^TTAAG 3089 CTTAAG AlwNI 103 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG 2932 CAGAATCTG Aor51HI 1350 AGCGCT AGC^GCT 1861 AGCGCT AvrII 86 CCTAGG C^CTAGG 244 CCTAGG BalI 540 TGGCCA TGG^CCA 3622 TGGCCA BamHI 577 GGATCC G^GATCC 2664 GGATCC BfrI 1966 CTTAAG C^TTAAG 3089 CTTAAG BlnI 86 CCTAGG C^CTAGG 244 CCTAGG BlpI 1857 GCTCAGC GC^T[ACGT]AGC 4304 GCTGAGC Bpu1102I 1857 GCTCAGC GC^T[ACGT]AGC 4304 GCTGAGC BsaAI 133 CACGTA [CT]AC^GT[GA] 4006 CACGTG BsaBI 2605 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2967 GATGTAGATC BsiWI 1230 CGTACG C^GTACG 1566 CGTACG BspMI 855 ACCTGC ACCTGC(4/8) 2290 GCAGGT BsrDI 3886 CATTGC GCAATG(2/0) 3958 CATTGC BssHII 3442 GCGCGC G^CGCGC 3444 GCGCGC BstXI 3370 CCAGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4228 CCAGCTGGGTGG CelII 1857 GCTCAGC GC^T[ACGT]AGC 4304 GCTGAGC DraI 3465 TTTAAA TTT^AAA 4545 TTTAAA DrdI 854 GACCTGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3159 GACACTCAGGTC DsaI 797 CCGTGG C^C[GA][CT]GG 2581 CCATGG Eco47III 1350 AGCGCT AGC^GCT 1861 AGCGCT HaeII 1350 AGCGCT [GA]GCGC^[CT] 1861 AGCGCT HgaI 958 GACGC GACGC(5/10) 1042 GACGC MamI 2605 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2967 GATGTAGATC MluNI 540 TGGCCA TGG^CCA 3622 TGGCCA MscI 540 TGGCCA TGG^CCA 3622 TGGCCA NruI 176 TCGCGA TCG^CGA 708 TCGCGA Psp1406I 126 AACGTT AA^CGTT 304 AACGTT PvuII 3628 CAGCTG CAG^CTG 4229 CAGCTG SacI 1771 GAGCTC GAGCT^C 1855 GAGCTC SapI 722 GAAGAGC GCTCTTC(1/4) 1588 GAAGAGC SphI 2896 GCATGC GCATG^C 4353 GCATGC StuI 765 AGGCCT AGG^CCT 2196 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI Alw44I AlwI ApaLI ApoI Asp700I AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BsrBI BsrGI BsrI BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI