##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Aor51HI ApaI AseI AsnI
Asp718I AviII AvrII BbeI BcgI
BfrI BglI BlnI BseAI BsiEI
BsiWI BsmI BspEI BsrBI Bst1107I
BstBI BstEII BstPI BstZ17I CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I FseI FspI KasI KpnI
MfeI MluI MroI MunI NarI
NdeI NotI NspV PacI PflMI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SmaI SpeI
Sse8387I SwaI Van91I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AcyI 2275 GACGCC G[GA]^CG[CT]C
AhdI 3790 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AscI 138 GGCGCGCC GG^CGCGCC
AspEI 3790 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 309 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3198 TGGCCA TGG^CCA
BamHI 3741 GGATCC G^GATCC
BbvCI 2309 GCTGAGG CCTCAGC(-5/-2)
BciVI 1000 GTATCC GTATCC(6/5)
BclI 3147 TGATCA T^GATCA
BsaBI 893 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2275 GACGCC G[GA]^CG[CT]C
BsaI 2209 GGTCTC GGTCTC(1/5)
BsaWI 6 ACCGGA [AT]^CCGG[AT]
BspDI 3682 ATCGAT AT^CGAT
BspLU11I 4557 ACATGT A^CATGT
BssHII 139 GCGCGC G^CGCGC
BssSI 1182 CACGAG CACGAG(-5/-1)
BstXI 1672 CCACGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 3682 ATCGAT AT^CGAT
EaeI 3198 TGGCCA [CT]^GGCC[GA]
Eam1105I 3790 GACTGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1652 CCTTGGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 4585 GATATC GAT^ATC
EcoT22I 395 ATGCAT ATGCA^T
FbaI 3147 TGATCA T^GATCA
Hin1I 2275 GACGCC G[GA]^CG[CT]C
HindIII 3416 AAGCTT A^AGCTT
KspI 4338 CCGCGG CCGC^GG
MamI 893 GATTAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3198 TGGCCA TGG^CCA
MscI 3198 TGGCCA TGG^CCA
NruI 709 TCGCGA TCG^CGA
NsiI 395 ATGCAT ATGCA^T
PflFI 309 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3273 GACTGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1664 CTGCAG CTGCA^G
SacII 4338 CCGCGG CCGC^GG
SapI 726 GAAGAGC GCTCTTC(1/4)
SgrAI 13 CGCCGGCG C[GA]^CCGG[CT]G
SnaBI 3904 TACGTA TAC^GTA
SphI 3647 GCATGC GCATG^C
SspI 3554 AATATT AAT^ATT
Tth111I 309 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1717 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
Alw44I 290 GTGCAC G^TGCAC
3238 GTGCAC
ApaLI 290 GTGCAC G^TGCAC
3238 GTGCAC
Asp700I 1584 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3595 GAAACACTTC
BanI 2497 GGTGCC G^G[CT][GA]CC
3474 GGTGCC
BglII 3107 AGATCT A^GATCT
3661 AGATCT
BsmBI 438 GAGACG CGTCTC(1/5)
778 GAGACG
BspHI 2885 TCATGA T^CATGA
3778 TCATGA
BsrDI 4299 CATTGC GCAATG(2/0)
3390 GCAATG
BsrFI 14 GCCGGC [GA]^CCGG[CT]
115 GCCGGC
Cfr10I 14 GCCGGC [GA]^CCGG[CT]
115 GCCGGC
DraI 3563 TTTAAA TTT^AAA
4637 TTTAAA
DraIII 2702 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
4645 CACCAAGTG
EcoRI 786 GAATTC G^AATTC
4445 GAATTC
HpaI 1739 GTTAAC GTT^AAC
2375 GTTAAC
NaeI 14 GCCGGC GCC^GGC
115 GCCGGC
NcoI 1276 CCATGG C^CATGG
4275 CCATGG
NgoMIV 14 GCCGGC G^CCGGC
115 GCCGGC
NheI 3828 GCTAGC G^CTAGC
3958 GCTAGC
PaeR7I 175 CTCGAG C^TCGAG
4304 CTCGAG
RcaI 2885 TCATGA T^CATGA
3778 TCATGA
StuI 1265 AGGCCT AGG^CCT
1820 AGGCCT
XcmI 1243 CCATGCTGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1883 CCAGCTCTGCTCTGG
XhoI 175 CTCGAG C^TCGAG
4304 CTCGAG
XmnI 1584 GAAGATGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3595 GAAACACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BanII
BbrPI BbsI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrGI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI Eco81I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PmaCI PmlI PpuMI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII